RESUMEN

The objective of this study was to identify the presence of Mycobacterium tuberculosis complex bacterial DNA in samples extracted from fresh cheeses; 95 samples of fresh cheese were obtained from municipal markets in the state of Hidalgo, in central Mexico, and were analyzed in triplicate. The exogenous control for the amplification was the mitochondrial gene for cytochrome b (cyt-b). M. tuberculosis complex DNA was detected by nested-PCR amplification of a fragment of the mpb70 gene in six samples, four of which were obtained from regions with enzootic bovine tuberculosis. These results suggest that cheeses prepared with raw milk contaminated with M. bovis are being sold and consumed by humans, which may cause tuberculosis.

Asunto(s)

Queso/microbiología , Contaminación de Alimentos/análisis , Leche/microbiología , Mycobacterium tuberculosis/aislamiento & purificación , Animales , Proteínas Bacterianas/metabolismo , Bovinos , Queso/economía , Citocromos b/genética , México , Mycobacterium tuberculosis/clasificación , Mycobacterium tuberculosis/genética , Reacción en Cadena de la Polimerasa , Tuberculosis Bovina/microbiología