RESUMEN
Pulpotomy in deciduous teeth is a controversial issue, especially with regard to alternative materials used for the direct pulp capping of the root canal pulp tissue. The aim of the present study was to perform a histological analysis of the initial reaction of the root canal pulp tissue in rats, following pulpotomy and pulp capping with (1) green propolis extract, (2) iodoform paste, (3) green propolis extract + iodoform and (4) calcium hydroxide paste with saline solution. Analyses were performed after 24 hours, 72 hours and 7 days. The substances containing green propolis extract and iodoform led to the production of an intense inflammatory infiltrate and necrosis in the root canal pulp tissue throughout the analyses. In the calcium hydroxide group, inflammatory infiltrate only prevailed at the 72-hour evaluation. Among the substances tested, calcium hydroxide paste induced the lowest intensity of inflammatory response in the root canal pulp tissue. Longer studies should be carried out to analyze the pulp repair process following pulpotomy and pulp capping with the compounds analyzed.
Asunto(s)
Animales , Masculino , Ratas , Antiinfecciosos/uso terapéutico , Pulpa Dental/efectos de los fármacos , Inflamación/inducido químicamente , Própolis/uso terapéutico , Materiales de Recubrimiento Pulpar y Pulpectomía/uso terapéutico , Hidróxido de Calcio/uso terapéutico , Recubrimiento de la Pulpa Dental/métodos , Necrosis de la Pulpa Dental/inducido químicamente , Necrosis de la Pulpa Dental/patología , Hidrocarburos Yodados/uso terapéutico , Inflamación/patología , Pulpotomía/métodos , Ratas Wistar , Factores de TiempoRESUMEN
Pulpotomy in deciduous teeth is a controversial issue, especially with regard to alternative materials used for the direct pulp capping of the root canal pulp tissue. The aim of the present study was to perform a histological analysis of the initial reaction of the root canal pulp tissue in rats, following pulpotomy and pulp capping with (1) green propolis extract, (2) iodoform paste, (3) green propolis extract + iodoform and (4) calcium hydroxide paste with saline solution. Analyses were performed after 24 hours, 72 hours and 7 days. The substances containing green propolis extract and iodoform led to the production of an intense inflammatory infiltrate and necrosis in the root canal pulp tissue throughout the analyses. In the calcium hydroxide group, inflammatory infiltrate only prevailed at the 72-hour evaluation. Among the substances tested, calcium hydroxide paste induced the lowest intensity of inflammatory response in the root canal pulp tissue. Longer studies should be carried out to analyze the pulp repair process following pulpotomy and pulp capping with the compounds analyzed.
Asunto(s)
Antiinfecciosos/uso terapéutico , Pulpa Dental/efectos de los fármacos , Inflamación/inducido químicamente , Própolis/uso terapéutico , Materiales de Recubrimiento Pulpar y Pulpectomía/uso terapéutico , Animales , Hidróxido de Calcio/uso terapéutico , Recubrimiento de la Pulpa Dental/métodos , Necrosis de la Pulpa Dental/inducido químicamente , Necrosis de la Pulpa Dental/patología , Hidrocarburos Yodados/uso terapéutico , Inflamación/patología , Masculino , Pulpotomía/métodos , Ratas , Ratas Wistar , Factores de TiempoRESUMEN
PURPOSE: This study's purpose was to histologically evaluate the repair of rat tissue after pulpotomy and covering the pulp tissue with Copaifera langsdorffi oil resin, green propolis extract, fibrin sponge and iodoform-based paste. METHODS: Pulpotomies were performed in the maxillary and mandibular first molars of 21 Wistar rats (84 total teeth). The access cavities were sealed with Coltosol, and histological evaluations were performed at 24 hours, 15 days, and 30 days postoperatively. RESULTS: For all experimental periods and materials, an inflammatory response constituted predominantly by neutrophils was observed, being of milder intensity for the Copaifera langsdorffii oil-resin group and more severe for the fibrin sponge group, which exhibited periapical microabscesses. Necrosis was observed in all groups, and its extension increased with time, except for teeth protected with Copaifera langsdorffii oil-resin. Formation of a mineralized tissue barrier in the pulp exposure area occurred only in the Copaifera langsdorffii oil-resin group. Other findings, such as vascular congestion, edema, and hemorrhage, were observed in all cases. CONCLUSIONS: The inflammatory response was less severe, the area of pulp necrosis was smaller, and more frequent formation of a mineralized tissue barrier was noted after pulpotomy was performed with Copaifera langsdorffii oil-resin compared to the other materials tested.
Asunto(s)
Pulpa Dental/fisiología , Fabaceae , Fitoterapia , Extractos Vegetales/uso terapéutico , Aceites de Plantas/uso terapéutico , Materiales de Recubrimiento Pulpar y Pulpectomía/uso terapéutico , Animales , Necrosis de la Pulpa Dental/etiología , Dentina Secundaria/metabolismo , Femenino , Adhesivo de Tejido de Fibrina/uso terapéutico , Hidrocarburos Yodados/uso terapéutico , Absceso Periapical/etiología , Própolis/uso terapéutico , Pulpitis/etiología , Pulpotomía/efectos adversos , Distribución Aleatoria , Ratas , Ratas WistarRESUMEN
Objetivo: Avaliar a atividade antimicrobiana da própolis e de outras substâncias já utilizadas na proteção pulpar de dentes decíduos, contra cepas de Enterococcus faecalis. Método: Utilizou-se a técnica de difusão em ágar, pelo método do poço, sendo analisadas 11 substâncias: 1) Extrato de própolis verde; 2) Pasta Guedes-Pinto (Iodofórmio + PMCC + Rifocort®); 3)Extrato de própolis verde + Rifocort® + Iodofórmio; 4) Rifocort® +Extrato de própolis verde; 5) Hidróxido de cálcio + Extrato de própolis verde; 6) Hidróxido de cálcio + Soro fisiológico; 7) Iodofórmio +Extrato de própolis verde; 8) Iodofórmio + Soro fisiológico; 9)Rifocort®; 10) Paramonoclorofenol canforado e 11) Soro fisiológico(controle negativo). Resultados: O Rifocort®; o Rifocort® com extrato de própolis verde; a pasta Guedes-Pinto; a pasta de própolis verde com Rifocort® eiodofórmio; e o paramonoclorofenol canforado apresentaram ação antimicrobiana contra o E. faecalis significativamente superior quando comparadas com as demais substâncias testadas. A pasta de hidróxido de cálcio com própolis verde, a solução de extrato de própolis verde, a pasta de iodofórmio com solução de própolis verde apresentaram menor atividade antimicrobiana, não havendo diferença estatisticamente significativa entre estas três formulações. O soro fisiológico, a pasta de hidróxido de cálcio com soro fisiológico e a pasta de iodofórmio com soro fisiológico não apresentaram atividade antimicrobiana. Conclusão: A solução de extrato de própolis verde apresentou baixa atividade antimicrobiana contra o E. faecalis e, dentre as substâncias testadas, o Rifocort® apresentou maior ação antimicrobiana.