RESUMEN
This research aims to examine the impact of programmed cell death 4 (PDCD4), microRNA-21 (miR-21) and microRNA-208a (miR-208a) transcripts, and protein levels on oral epithelial dysplasia (OED) development in oral squamous cell carcinoma (OSCC). METHODS: The research investigation involved the collection of saliva, blood, and tissue samples from a total of 20 patients diagnosed with OSCC, 15 patients diagnosed with OED, and 15 healthy individuals. PDCD4, miR-21, and miR-208a expression was performed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). PDCD4 protein levels were assessed using enzyme-linked immunosorbent assay (ELISA) in both saliva and blood samples. For statistical analysis, the Kruskal-Wallis test and the Spearmen rank test were utilised. RESULTS: PDCD4 expression levels were considerably lower in patients with OSCC and OED (p < 0.05) in three biological samples. In contrast, miR-21 expression was higher in OED and OSCC patients. Patients with low PDCD4 mRNA levels and strong miR-21 expression had a significant connection (p < 0.05) with tumor size and depth. CONCLUSIONS: Examining PDCD4 and miR-21 transcript levels may help detect the transition from OED to OSCC. This work suggests that PDCD4 and miR-21 expression levels in liquid biopsies may be biomarkers for OED monitoring in the future.
Asunto(s)
Proteínas Reguladoras de la Apoptosis , Biomarcadores de Tumor , Carcinoma de Células Escamosas , MicroARNs , Neoplasias de la Boca , Proteínas de Unión al ARN , Humanos , MicroARNs/sangre , MicroARNs/genética , Neoplasias de la Boca/genética , Neoplasias de la Boca/sangre , Neoplasias de la Boca/patología , Proteínas de Unión al ARN/genética , Proteínas Reguladoras de la Apoptosis/genética , Masculino , Biomarcadores de Tumor/genética , Biomarcadores de Tumor/sangre , Femenino , Carcinoma de Células Escamosas/genética , Persona de Mediana Edad , Biopsia Líquida , Saliva/química , Saliva/metabolismo , Anciano , Adulto , Estudios de Casos y Controles , Lesiones Precancerosas/genética , Lesiones Precancerosas/patologíaRESUMEN
In the present work, polynomial, discrete singular convolution and sinc quadrature techniques are employed as the new techniques to derive accurate and efficient numerical solutions for the reaction-diffusion equations. Three models, Fitzhugh-Nagumo, Newell-Whitehead-Segel, and tumor growth models, were presented. The equations of three models are reduced to nonlinear ordinary differential equations by using different quadrature schemes. Then, Runge-Kutta fourth-order method is employed to solve nonlinear ordinary differential equations. In addition, the MATLAB program is used to solve these problems. Comparisons between the new methods and the existing ones are included, demonstrating the ease of implementation and efficiency. Also, the calculated results are supported by four various statistical errors. It is found that the rate of error reaches ≤ 10-6 in discrete singular convolution depending on regularized Shannon kernel which is better than others. Further, a parametric analysis is presented to discuss the influence of diffusion and reaction parameters on the solution.
RESUMEN
Squamous cell carcinoma (SCC) of the renal pelvis is a particularly rare tumor that accounts for a minor portion of renal malignancies and is aggressive with an unfavorable prognosis. It is usually diagnosed after surgery and at advanced stages as it does not possess specific clinical and radiological properties. The pathological examination of a 38-year-old female patient who had undergone nephrectomy due to a nonfunctioning right kidney caused by long-standing staghorn calculus revealed moderately differentiated renal pelvis SCC invading the renal parenchyma. The patient who experienced severe lumbar pain in the second postoperative month presented lymphadenopathy, which could not be detected with ultrasonography but was diagnosed with 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18FDG PET/CT). The patient received systemic treatment following the early diagnosis and survived past the average survival time. It was concluded that in cases where SCC was diagnosed after nephrectomy, investigating metastasis with 18FDG PET/CT and initiating early systemic treatment in the presence of metastasis could contribute to survival.
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Carcinoma de Células Renales/patología , Carcinoma de Células Escamosas/patología , Neoplasias Renales/patología , Pelvis Renal/diagnóstico por imagen , Tomografía Computarizada por Tomografía de Emisión de Positrones/métodos , Adulto , Carcinoma de Células Renales/cirugía , Carcinoma de Células Escamosas/cirugía , Resultado Fatal , Femenino , Fluorodesoxiglucosa F18 , Humanos , Neoplasias Renales/cirugía , Pelvis Renal/patología , Linfadenopatía , Masculino , Estadificación de Neoplasias , Nefrectomía , RadiofármacosRESUMEN
BACKGROUND: Male circumcision (MC) is one of the most commonly used surgical procedures worldwide for medical and traditional reasons. No studies have compared the postoperative pain advantages of conventional techniques (i.e., sleeve and dorsal slit). OBJECTIVE: In this prospective randomized double-blind study, we investigated the effect of two surgical techniques (i.e., sleeve and dorsal slit) on postoperative pain and emergence agitation. STUDY DESIGN: This prospective study was conducted between January and July 2013. Approval was obtained from the local Ethical Committee on 17 December 2012, 06/23 (CLINICAL TRIALS IDENTIFIER: NCT 01909765). We compared two surgical techniques (i.e., the dorsal slit incision technique (Group A) and the double incision (i.e., sleeve) technique (Group B) in 60 children who were subjected to MC surgery under general anesthesia. All children received dorsal nerve blocks with bupivacaine. The modified objective pain scale (MOPS) was used for pain assessment, and the Ramsey Sedation Scale was used for the assessment of agitation during anesthesia emergence. RESULTS: The MOPS scores were lower in Group B than in Group A in the post-anesthesia care unit and during the 4th hour post-surgery (p = 0.01 and p = 0.037, respectively). Twelve children (40%) in Group A and 23 children (76.6%) in Group B required no additional analgesia on postoperative day one (p = 0.004). The Ramsey sedation scores were lower in Group A (p = 0.018). DISCUSSION: Dorsal slit is often the primary method in cases with paraphimosis; during this procedure, the frenulum frequently cannot be preserved at the 6-o'clock position of the mucosa, because of traction applied to skin and mucosa. As a result, the frenular artery is injured. In contrast, the sleeve technique protects the frenulum and the anatomic structures of the glans. In the sleeve technique, providing hemostasis and preventing partial ischemia by protecting the frenular artery reduces postoperative pain and complications. This present study demonstrated that the sleeve technique, which preserved the frenular artery, caused less bleeding, reduced electrocautery use and less ischemia than the dorsal slit technique. The sleeve technique effectively reduces early postoperative pain and agitation after circumcision, provided that adequate postoperative analgesia has been achieved. While all variables except the employed surgical techniques were similar, Group B had advantages with respect to analgesic requirement and pain control during the first 8 h after the operation. CONCLUSION: The sleeve technique provides lower pain scores and a reduced incidence of agitation after elective MC.
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Circuncisión Masculina/métodos , Dolor Postoperatorio/prevención & control , Analgésicos/uso terapéutico , Niño , Preescolar , Circuncisión Masculina/efectos adversos , Método Doble Ciego , Humanos , Masculino , Dimensión del Dolor , Dolor Postoperatorio/diagnóstico , Dolor Postoperatorio/etiología , Estudios ProspectivosRESUMEN
BACKGROUND: The aim of the study was to investigate the effect of preoperative ultrasound-guided (US) intercostal nerve block (ICNB) in the 11th and 12th intercostal spaces on postoperative pain control and tramadol consumption in patients undergoing percutaneous nephrolithotomy (PCNL). METHODS: After obtaining ethical committee approval and written informed patient consent, 40 patients were randomly allocated to the ICNB group or the control group. For the ICNB group US-guided ICNB was performed with 0.5 % bupivacaine and 1/200,000 epinephrine at the 11th and 12th intercostal spaces after premedication. A sham block was performed for the control group and postoperative pain and tramadol consumption were recorded by anesthesiologists blinded to the treatment. RESULTS: Postoperative visual analog scale scores at all follow-up times were found to be significantly lower in the ICNB group than in the control group (p < 0.05). The mean 24 h intravenous tramadol consumption was 97.5 ± 39.5 mg for the ICNB group which was significantly lower than the 199.7 ± 77.6 mg recorded for the control group (p < 0.05). CONCLUSION: In PCNL with nephrostomy tube placement US-guided ICNB performed at the 11th and 12th intercostal spaces provided effective analgesia.
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Nervios Intercostales/diagnóstico por imagen , Nefrostomía Percutánea/métodos , Bloqueo Nervioso/métodos , Dolor Postoperatorio/epidemiología , Ultrasonografía Intervencional/métodos , Adolescente , Adulto , Anciano , Analgésicos Opioides/administración & dosificación , Analgésicos Opioides/uso terapéutico , Determinación de Punto Final , Femenino , Humanos , Masculino , Persona de Mediana Edad , Nefrostomía Percutánea/efectos adversos , Dimensión del Dolor , Satisfacción del Paciente , Complicaciones Posoperatorias/epidemiología , Estudios Prospectivos , Tramadol/administración & dosificación , Tramadol/uso terapéutico , Adulto JovenRESUMEN
BACKGROUND AND AIMS: To evaluate the anxiety, depression and related psychogenic erectile dysfunction that might be developed before and after pacemaker implantation in patients with cardiac arrhythmias. MATERIAL AND METHODS: Thirty permanent pacemaker implanted male patients, were enrolled to study between September 2006 and September 2008. Erectile function domain questions of International Index of Erectile Function (IIEF-6) and Hospital Anxiety and Depression Scale (HAD) questionnaires were applied to patients, 6 months before pacemaker implantation (BP6) and on month 1 (AP1) and 6 after application (AP6). Patients were included in a multidisciplinary cardiac rehabilitation-adaptation program with a duration of 1-2 months. Patients were evaluated in subgroups. RESULTS: Mean age was 51.5 ± 10.3. Most frequent diagnosis was observed as AV block in etiology. The mean IIEF values were changed 22.8â20.2â24.6 in BP6, AP1 and AP6 time frames consecutively. However, the mean HAD-Anxiety scores were evaluated as 8.1â17.0â7.3 and the mean HAD-Depression as 3.9â7.9â8.9 consecutively in the same time frames. CONCLUSION: Cardiac arrhythmia plus permanent pacemaker implantation, increased anxiety and depression of patients and decreased erectile function at AP1; however, the improvement in cardiac symptoms at AP6 with the possible positive effects of rehabilitation program, helps to reduce anxiety and increased IIEF scores, although there was still a slight increase in depression levels.
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Arritmias Cardíacas/terapia , Disfunción Eréctil/diagnóstico , Marcapaso Artificial/psicología , Ansiedad/etiología , Depresión/etiología , Disfunción Eréctil/psicología , Humanos , Masculino , Persona de Mediana Edad , Escalas de Valoración PsiquiátricaRESUMEN
Benign tumors of the proximal ureter are very rare. Many of them could be confused with urothelial carcinoma and unnecessarily treated by nephrectomy. In this case, we present the treatment of a lymphangioma localized in the upper ureter, which is an example of benign tumor. During treatment percutaneous tumor resection, an organ-sparing approach, was employed.
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Pelvis Renal/cirugía , Linfangioma/diagnóstico , Neoplasias Ureterales/diagnóstico , Adulto , Femenino , Humanos , Pelvis Renal/patología , Procedimientos Quirúrgicos Mínimamente Invasivos/métodos , Uréter/patologíaRESUMEN
OBJECTIVE: To evaluate the effectiveness and tolerability of intravesical epirubicin treatment to prevent recurrence and progression of superficial bladder tumor after TUR. METHODS: 44 patients with superficial bladder tumor, 28 primary and 16 recurrent cases were given 50 mg intravesical epirubicin once a week for 8 weeks after TUR. Recurrent cases had not been treated by any intravesical agent before. Follow-up was done by cystoscopy, urine cytology and random biopsies. RESULTS: Local recurrence after treatment was found in 14.28% and 18.75%, and recurrence in another area was found in 21.42% and 31.25% of the primary and recurrent tumor groups respectively. Total recurrence in both groups was 40.91%. No systemic toxicity was observed and local side effects were treated symptomatically. CONCLUSION: Intravesical epirubicin after TUR for superficial bladder tumors especially for the primary ones, is a reliable method that provides effective prophylaxis with tolerable side effects.
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Antibióticos Antineoplásicos/administración & dosificación , Carcinoma de Células Escamosas/prevención & control , Epirrubicina/administración & dosificación , Recurrencia Local de Neoplasia/prevención & control , Neoplasias de la Vejiga Urinaria/prevención & control , Administración Intravesical , Adulto , Anciano , Carcinoma de Células Escamosas/cirugía , Quimioterapia Adyuvante , Femenino , Humanos , Masculino , Persona de Mediana Edad , Recurrencia Local de Neoplasia/epidemiología , Cuidados Posoperatorios , Uretra , Neoplasias de la Vejiga Urinaria/cirugía , Procedimientos Quirúrgicos UrológicosRESUMEN
An 8 1/2-month-old girl with biliary atresia and polysplenia syndrome having multiple vascular anomalies without cardiac anomalies is reported. Interruption of the inferior vena cava with azygous continuation, which is a common anomaly, was seen in conjunction with origin of the common hepatic artery from the superior mesenteric artery and with a circumaortic renal vein. The case has particular importance in that no hepatic artery or renal vein variations have been described with biliary atresia and polysplenia syndrome in the literature thus far to our knowledge. The anomalies were shown using different radiological examinations including computed tomography, echocardiography, angiography, venography and magnetic resonance imaging.
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Anomalías Múltiples , Arteria Hepática/anomalías , Arteria Mesentérica Superior/anomalías , Venas Renales/anomalías , Bazo/anomalías , Anomalías Múltiples/patología , Atresia Biliar , Femenino , Arteria Hepática/patología , Humanos , Lactante , Arteria Mesentérica Superior/patología , Venas Renales/patología , Bazo/patología , SíndromeRESUMEN
Twenty-nine patients with two-, three- and four-part proximal humeral fractures were treated by two-prong splint. At the latest follow-up, 22 patients had excellent and satisfactory results according to the Neer criteria, with only two failures. Avascular necrosis was detected in only one patient. Given these results, we conclude that the present method can be the treatment of choice for these injuries.
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Fracturas del Hombro/terapia , Férulas (Fijadores) , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Diseño de Equipo , Femenino , Humanos , Masculino , Persona de Mediana Edad , Radiografía , Fracturas del Hombro/diagnóstico por imagenRESUMEN
To evaluate the early and late effects of extracorporeal shock wave lithotripsy on renal function, we prospectively designed a controlled study using a Direx lithotriptor. Twenty-five patients with renal stones and 16 healthy volunteers as the control group were included in the study. Blood and urine samples were collected before and after 24 hours, seven days and 8 months in the patient group. White blood cell count, serum levels of haemoglobin, urea, creatinine, SGOT, SGPT, AP, and LDH were determined. 24-hour urine specimens were collected to be tested for volume, excretion of creatinine, albumin, N-acetyl-beta-glucosaminidase, gamma-glutamyltransferase and beta-2-microglobulin. There were statistically significant increments in the secretion of urinary enzymes and albumin in the early period after ESWL, no longer lasting 8 months after the procedure. At 8 months one patient was hypertensive as judged by the diastolic pressure above 95 mm Hg. The results of this study showed that, although there was a transient glomerular and tubular damage after extracorporeal shock wave lithotripsy, the procedure seems safe and causes no permanent deterioration in renal function.
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Cálculos Renales/fisiopatología , Cálculos Renales/cirugía , Glomérulos Renales/fisiopatología , Túbulos Renales/fisiopatología , Litotricia , Adulto , Femenino , Humanos , Cálculos Renales/metabolismo , Pruebas de Función Renal , Masculino , Persona de Mediana Edad , Estudios ProspectivosRESUMEN
The close proximity of the prostate, vas deferens, seminal vesicles and ejaculatory ducts are a diagnostic challenge to the cysts located in that region. Three patients with diverticulum of the ejaculatory ducts, 2 with prostate cysts and 1 patient with seminal vesicle cyst are presented. Transrectal ultrasound, computed tomography and magnetic resonance imaging together with aspiration of the cyst establish diagnosis and probably prevent unnecessary exploration.