RESUMEN
The fractional composition of chromatin proteins and an electron-microscopic pattern of cell ultrastructure of the Ehrlich ascite tumours and ascite lymphoma NK/Ly were studied when suppressing their growth by a combined action of cyclophosphane and polyene antibiotic. Changes in the fractional composition of chromatin proteins were found under the effect of levorin. A sharp increase in this effect and summation of heterogeneous morphological changes provoked by each of the preparation occur under a combined application of the preparations.
Asunto(s)
Antifúngicos/administración & dosificación , Candicidina/administración & dosificación , Carcinoma de Ehrlich/metabolismo , Ciclofosfamida/administración & dosificación , Linfoma/metabolismo , Animales , Candicidina/farmacología , Carcinoma de Ehrlich/tratamiento farmacológico , Carcinoma de Ehrlich/ultraestructura , Cromatina/metabolismo , Ciclofosfamida/farmacología , Quimioterapia Combinada , Femenino , Histonas/metabolismo , Linfoma/tratamiento farmacológico , Linfoma/ultraestructura , Masculino , Ratones , Proteínas de Neoplasias/metabolismoRESUMEN
The fractional composition of the proteins of the plasmatic membranes of the Ehrlich's tumor cells treated with various drugs was studied with the use of electrophoresis in polyacrylamide gel. It was shown that the character of the changes in the fractional composition of the plasmatic membrane proteins under the effect of levorin and cyclophosphamide was different. When the drugs were used in combination, summation of the changes induced by every drug alone was observed. Possible causes of the changes in the fractional composition of the membrane proteins are discussed.
Asunto(s)
Antifúngicos/uso terapéutico , Candicidina/uso terapéutico , Carcinoma de Ehrlich/análisis , Ciclofosfamida/uso terapéutico , Proteínas de la Membrana/análisis , Proteínas de Neoplasias/análisis , Animales , Carcinoma de Ehrlich/tratamiento farmacológico , Evaluación Preclínica de Medicamentos , Sinergismo Farmacológico , Quimioterapia Combinada , RatonesRESUMEN
The ultrastructure of cells of mouse ascites lymphoma was studied by transmission electron microscopy. It was established that cyclophosphamide treatment (6 days) reduces cell concentration in ascitic fluid and alters tumor cell ultrastructure. Apart from dystrophic and necrotized ones, there are cells exhibiting the signs of active protein synthesis. Viral bodies in tumor cell cytoplasm were observed both in experimental and control animals.