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El síndrome de erupción medicamentosa con eosinofilia y síntomas sistémicos (drug reaction with eosinophilia and systemic symptoms, DRESS), también conocido como síndrome de hipersensibilidad inducida por medicamentos, es una reacción rara potencialmente mortal que causa una erupción grave y que puede provocar insuficiencia multiorgánica. Como con otras erupciones medicamentosas graves, los linfocitos T específicos para un medicamento tienen una función crucial en el síndrome DRESS. El modelo de hapteno/pro-hapteno, el modelo de interacción farmacológica y el modelo alterado de repertorio de péptidos son tres modelos diferentes desarrollados para describir la relación/interacción entre un medicamento o sus metabolitos y el sistema inmunitario. Analizamos nuestra experiencia con el tratamiento con ciclosporina en un caso de síndrome DRESS resistente a esteroides causado por ácido valproico en una niña y sus resultados clínicos, de laboratorio y de antígeno leucocitario humano (HLA).

Drug reaction with eosinophilia and systemic symptoms (DRESS), also known as drug-induced hypersensitivity syndrome, is a potentially life-threatening rare reaction that causes a severe rash and can lead to multiorgan failure. As in other severe drug eruptions, drug-specific T lymphocytes play a crucial role in DRESS. The hapten/pro-hapten model, pharmacological interaction model, and altered peptide repertoire model are three different models developed to describe the relationship/interaction between a medication or its metabolites and the immune system. We discuss our experience with cyclosporine treatment in a steroid-resistant DRESS syndrome caused by valproic acid in a girl, as well as her clinical, laboratory, and human leukocyte antigens (HLA) study results

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Drug reaction with eosinophilia and systemic symptoms (DRESS), also known as drug-induced hypersensitivity syndrome, is a potentially life-threatening rare reaction that causes a severe rash and can lead to multiorgan failure. As in other severe drug eruptions, drug-specific T lymphocytes play a crucial role in DRESS. The hapten/pro-hapten model, pharmacological interaction model, and altered peptide repertoire model are three different models developed to describe the relationship/interaction between a medication or its metabolites and the immune system. We discuss our experience with cyclosporine treatment in a steroid-resistant DRESS syndrome caused by valproic acid in a girl, as well as her clinical, laboratory, and human leukocyte antigens (HLA) study results.

El síndrome de erupción medicamentosa con eosinofilia y síntomas sistémicos (drug reaction with eosinophilia and systemic symptoms, DRESS), también conocido como síndrome de hipersensibilidad inducida por medicamentos, es una reacción rara potencialmente mortal que causa una erupción grave y que puede provocar insuficiencia multiorgánica. Como con otras erupciones medicamentosas graves, los linfocitos T específicos para un medicamento tienen una función crucial en el síndrome DRESS. El modelo de hapteno/pro-hapteno, el modelo de interacción farmacológica y el modelo alterado de repertorio de péptidos son tres modelos diferentes desarrollados para describir la relación/interacción entre un medicamento o sus metabolitos y el sistema inmunitario. Analizamos nuestra experiencia con el tratamiento con ciclosporina en un caso de síndrome DRESS resistente a esteroides causado por ácido valproico en una niña y sus resultados clínicos, de laboratorio y de antígeno leucocitario humano (HLA).

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Objetivo. La alergia alimentaria es un problema de salud pública. Los objetivos fueron determinar la asociación entre la alergia a la proteína de la leche de vaca (APLV) y la vitamina 25(OH)D en los lactantes diagnosticados con APLV y la asociación entre la 25(OH)D y (a) tamaño de la zona indurada en la prueba intraepidérmica, (b) IgE específica para leche y (c) IgE específica para caseína. Métodos. Estudio de casos y controles prospectivo, observacional con niños < 2 años con APLV confirmada mediante prueba de exposición oral al alimento. Se incluyó a lactantes sanos como controles. La concentración sérica de 25(OH)D se obtuvo en ambos grupos. Se estudió la correlación entre la vitamina D y la APLV. Resultados. Entre los pacientes (n: 56), el 41,1 % (n: 23) tenía alergia mediada por la IgE y el 58,9 % (n: 33), alergia no mediada por la IgE. No hubo diferencias estadísticamente significativas entre los grupos con APLV y de referencia (n: 55) en la 25(OH)D (33,85 ± 16,18 ng/ml, 30,70 ± 14,90 ng/ml; respectivamente, p : 0,289). No hubo diferencias estadísticamente significativas entre los grupos según la 25(OH)D (adecuada, insuficiencia, deficiencia; p = 0,099). Con la prueba intraepidérmica, se determinó una correlación negativa sin significancia estadística entre la zona indurada del antígeno de la leche y la 25(OH)D (p: 0,794; r = -0,037). Conclusiones. No se observaron diferencias significativas en la 25(OH)D entre ambos grupos. Estos resultados no respaldan la solicitud de rutina de análisis de 25(OH)D en los lactantes con APLV.

Aim. Food allergy is an important public health concern with an increasing prevalence. The objectives were to determine the possible association between cow's milk protein allergy (CMPA) and 25(OH)D (vitamin-D) levels of infants with an initial diagnosis of CMPA and the association between 25(OH)D levels and (a) SPT (skin prick test) induration size, (b) specific IgE to milk, (c) specific IgE to casein. Methods. Prospective, observational, case control study; the study group was composed of children < 2 years of age with a diagnosis of CMPA confirmed by an oral food challenge test. Healthy infants were enrolled as controls. Serum 25(OH) D levels were obtained at the initial workup in both groups. The correlation of vitamin-D levels was investigated in the development of CMPA. Results. Among the study group of patients (n:56) 41,1% (n:23) had IgE-mediated and 58,9% (n:33) had non-IgE-mediated allergies There were no statistically significant differences between the CMPA and control groups (n: 55), in terms of serum 25(OH)D levels (33.85 ± 16.18ng/ml, 30.70 ± 14.90ng/ml; respectively, p:0.289). No statistically significant difference was found between study and controls according to 25(OH) D levels (adequate, insufficiency, deficiency; p=0.099). In the SPT of the CMPA group, a negative weak correlation without statistical significance was determined between the induration diameter of milk antigen and serum levels of 25(OH)D (p:0,794; r= -0,037). Conclusions. No significant difference was found in serum 25 (OH)D levels between infants with CMPA and healthy controls. Our results do not support the routine request for 25(OH)D levels in pediatric age CMPA patients at their initial workup.

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AIM: Food allergy is an important public health concern with an increasing prevalence. The objectives were to determine the possible association between cow's milk protein allergy (CMPA) and 25(OH)D (vitamin-D) levels of infants with an initial diagnosis of CMPA and the association between 25(OH)D levels and (a) SPT (skin prick test) induration size, (b) specific IgE to milk, (c) specific IgE to casein. METHODS: Prospective, observational, case control study; the study group was composed of children < 2 years of age with a diagnosis of CMPA confirmed by an oral food challenge test. Healthy infants were enrolled as controls. Serum 25(OH) D levels were obtained at the initial workup in both groups. The correlation of vitamin-D levels was investigated in the development of CMPA. RESULTS: Among the study group of patients (n:56) 41,1% (n:23) had IgE-mediated and 58,9% (n:33) had non-IgE-mediated allergies There were no statistically significant differences between the CMPA and control groups (n: 55), in terms of serum 25(OH)D levels (33.85 ± 16.18ng/ml, 30.70 ± 14.90ng/ml; respectively, p:0.289). No statistically significant difference was found between study and controls according to 25(OH) D levels (adequate, insufficiency, deficiency; p=0.099). In the SPT of the CMPA group, a negative weak correlation without statistical significance was determined between the induration diameter of milk antigen and serum levels of 25(OH)D (p:0,794; r= -0,037). CONCLUSIONS: No significant difference was found in serum 25 (OH)D levels between infants with CMPA and healthy controls. Our results do not support the routine request for 25(OH)D levels in pediatric age CMPA patients at their initial workup.

Objetivo. La alergia alimentaria es un problema de salud pública. Los objetivos fueron determinar la asociación entre la alergia a la proteína de la leche de vaca (APLV) y la vitamina 25(OH)D en los lactantes diagnosticados con APLV y la asociación entre la 25(OH)D y (a) tamaño de la zona indurada en la prueba intraepidérmica, (b) IgE específica para leche y (c) IgE específica para caseína. Métodos. Estudio de casos y controles prospectivo, observacional con niños < 2 años con APLV confirmada mediante prueba de exposición oral al alimento. Se incluyó a lactantes sanos como controles. La concentración sérica de 25(OH)D se obtuvo en ambos grupos. Se estudió la correlación entre la vitamina D y la APLV. Resultados. Entre los pacientes (n: 56), el 41,1 % (n: 23) tenía alergia mediada por la IgE y el 58,9 % (n: 33), alergia no mediada por la IgE. No hubo diferencias estadísticamente significativas entre los grupos con APLV y de referencia (n: 55) en la 25(OH)D (33,85 ± 16,18 ng/ml, 30,70 ± 14,90 ng/ ml; respectivamente, p : 0,289). No hubo diferencias estadísticamente significativas entre los grupos según la 25(OH)D (adecuada, insuficiencia, deficiencia; p = 0,099). Con la prueba intraepidérmica, se determinó una correlación negativa sin significancia estadística entre la zona indurada del antígeno de la leche y la 25(OH)D (p: 0,794; r = -0,037). Conclusiones. No se observaron diferencias significativas en la 25(OH)D entre ambos grupos. Estos resultados no respaldan la solicitud de rutina de análisis de 25(OH)D en los lactantes con APLV.

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The aim of this study was to determine the LD30 values of the essential oils of Origanum minutiflorum He Schwarz-PH Davis (OM) and Juniperus excelca by Bieb.subsp. Excel (JE) in vivo investigation on the effects of malondialdehyde (MDA), vitamin C and some biochemical parameters. In this, study the essential oils of OM and JE plants were used. LD50 values of the essential oils were determined by using rats. OM (n=10), JE (n=10), carvacrol (n=10) which dissolve in olive oil were used as experimental group and as control group saline (SF) (n=8) and solvent Olive oil (n=10) were used and applied intraperitoneal on rats for 12 days in LD30 dosages. In the end of 12 days, Vit-C, malondialdehyde (MDA) and routine biochemical analyses were studied on their heart bloods. The difference in the levels of MDA and Vit-C was found significant among the groups (p0.005). When the values are put to paired comparison, the differences between groups were found statistically significant (p<0.005).OM and JE has led to significant changes in all lipid peroxidation and enzyme levels.

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The aim of this study was to determine the LD30 values of the essential oils of Origanum minutiflorum He Schwarz-PH Davis (OM) and Juniperus excelca by Bieb.subsp. Excel (JE) in vivo investigation on the effects of malondialdehyde (MDA), vitamin C and some biochemical parameters. In this, study the essential oils of OM and JE plants were used. LD50 values of the essential oils were determined by using rats. OM (n=10), JE (n=10), carvacrol (n=10) which dissolve in olive oil were used as experimental group and as control group saline (SF) (n=8) and solvent Olive oil (n=10) were used and applied intraperitoneal on rats for 12 days in LD30 dosages. In the end of 12 days, Vit-C, malondialdehyde (MDA) and routine biochemical analyses were studied on their heart bloods. The difference in the levels of MDA and Vit-C was found significant among the groups (p<0.005). When the biochemical parameters of the groups were compared, all differences in all test were found significant (p<0.005) except for creatinine (p>0.005). When the values are put to paired comparison, the differences between groups were found statistically significant (p<0.005).OM and JE has led to significant changes in all lipid peroxidation and enzyme levels.(AU)

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