RESUMEN
A BODIPY-based fluorophore decorated with a gold specific reactive handle (e.g., 2-alkynylallyl alcohol) displayed a ratiometric fluorescence change in response to Au3+ ions with extraordinary selectivity over other competing metal species, including Hg2+, Cu2+, Zn2+ and Pd2+. By way of a gold-catalyzed intramolecular cyclisation-isomerisation reaction sequence, a BODIPY construct with an extended π-conjugation transformed into a new structure with a relatively short π-system. This unique chemical transformation was accompanied by, and resulted in, a dramatic shift in the emission and absorption wavelength, which could be monitored as distinct changes in the color of the solution's emission. Apart from its outstanding analytical performance in solution, including a quick response time (<10 s), unique specificity, a high-fold ratiometric change (62-fold), and a remarkably low detection limit (358 nM), the probe also proved useful in monitoring Au3+ ions in human cells and plants (e.g., Nicotiana benthamiana).
RESUMEN
Drawing upon an intramolecular cyclization/annulation reaction sequence mediated by Hg2+ ions, a BODIPY-based fluorescent probe decorated with an alkynyl hydrazone motif responds rapidly and selectively to Hg2+ ions, with a detection limit of 29â nM and a fluorescence turn-on ratio of 15-fold. With the addition of Hg2+ ions, the BODIPY-based alkynyl hydrazone transforms into a pyrazole ring to mediate a turn-on emission response clearly observable to the naked eye under visible light excitation.
Asunto(s)
Hidrazonas , Mercurio , Ciclización , Colorantes Fluorescentes , IonesRESUMEN
We devised a new generation of halogen-based triplet sensitisers comprising geminal dibromides at the vinyl backbone of a BODIPY fluorophore. Incorporating geminal dibromides into the π-conjugation of BODIPY enhanced intersystem crossing due to the heavy atom effect, which in turn improved the extent of excited triplet states.
Asunto(s)
Antineoplásicos/farmacología , Compuestos de Boro/farmacología , Bromuros/farmacología , Fotoquimioterapia , Fármacos Fotosensibilizantes/farmacología , Compuestos de Vinilo/farmacología , Células A549 , Antineoplásicos/química , Compuestos de Boro/química , Bromuros/química , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Ensayos de Selección de Medicamentos Antitumorales , Humanos , Estructura Molecular , Imagen Óptica , Fármacos Fotosensibilizantes/química , Compuestos de Vinilo/químicaRESUMEN
A fluorescent probe decorated with an alkynyl ester unit (e.g. propiolate) displayed a selective turn-on type fluorescent response towards cysteine. Following a sequential addition-cyclisation pathway mediated by the addition of cysteine, the pre-fluorescent dye rapidly transformed into a new structure and induced a fluorescent response clearly observable with the naked eye.
RESUMEN
A boron-dipyrromethene (BODIPY)-based fluorescent probe with a phosgene-specific reactive motif shows remarkable selectivity toward phosgene, in the presence of which the nonfluorescent dye rapidly transforms into a new structure and induces a fluorescent response clearly observable to the naked eye under ultraviolet light. Given that dynamic, a prototypical handheld phosgene detector with a promising sensing capability that expedites the detection of gaseous phosgene without sophisticated instrumentation was developed. The proposed method using the handheld detector involves a rapid response period suitable for issuing early warnings during emergency situations.
RESUMEN
Upon complexation with Au(I), a photoinactive BODIPY derivative was transformed into a highly photoactive triplet sensitizer. Along with high efficiency in singlet oxygen generation (ΦΔ = 0.84), the new BODIPY-Au(I) skeleton showed excellent photocytotoxic activity against cancer cell lines (EC50 = 2.5 nM).
Asunto(s)
Compuestos de Boro/química , Estructura Molecular , Fotoquimioterapia , Fármacos Fotosensibilizantes , Oxígeno SingleteRESUMEN
Recently, development of fluorescent nanoparticle-based probes for various bioimaging applications has attracted great attention. This work aims to develop a new type fluorescent nanoparticle conjugate and evaluate its cytotoxic effects on A549 and BEAS 2B cell lines. Throughout the study, ionically crosslinked chitosan nanoparticles (CNs) were conjugated with carboxylated 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY-COOH). The results of conjugates (BODIPY-CNs) were investigated with regard to their physic-chemical, optical, cytotoxic properties and cellular internalization. The morphology of BODIPY-CNs was found to be spherical in shape and quite uniform having average diameter of 70.25 ± 11.99 nm. Cytotoxicty studies indicated that although BODIPY-COOH itself was quite toxic on both A549- and BEAS 2B-treated cells, CNs increased the cell viability of both cell lines via conjugation to BODIPY-COOH fluorescent molecule up to 67% for A549 and 74% for BEAS 2B cells. These results may suggest a possible utilization of the new fluorescent nanoparticle-based probe for bioimaging in biology and medicine.
Asunto(s)
Bronquios/metabolismo , Quitosano/metabolismo , Colorantes Fluorescentes/metabolismo , Nanopartículas/metabolismo , Porfobilinógeno/análogos & derivados , Mucosa Respiratoria/metabolismo , Absorción Fisiológica , Bronquios/citología , Bronquios/efectos de los fármacos , Línea Celular , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Quitosano/efectos adversos , Quitosano/química , Diagnóstico por Imagen/efectos adversos , Dispersión Dinámica de Luz , Colorantes Fluorescentes/efectos adversos , Colorantes Fluorescentes/química , Humanos , Microscopía de Fuerza Atómica , Microscopía Confocal , Microscopía Electrónica de Rastreo , Nanopartículas/efectos adversos , Nanopartículas/química , Nanopartículas/ultraestructura , Tamaño de la Partícula , Porfobilinógeno/efectos adversos , Porfobilinógeno/química , Porfobilinógeno/metabolismo , Mucosa Respiratoria/citología , Mucosa Respiratoria/efectos de los fármacos , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
Drawing upon a consecutive amide coupling and intramolecular cyclisation pathway, a one-pot, straightforward synthetic route has been developed for a range of pyrazole fused γ-pyrone derivatives. The reaction mechanism proposed for the chemoselective formation of γ-pyranopyrazole is furthermore fully supported by experimental and computational studies.
RESUMEN
Using an irreversible intramolecular cyclisation pathway triggered by gold ions, a boron-dipyrromethene (BODIPY) based fluorescent probe integrated with a reactive Z-enynol motif responds selectively to gold ions. With the addition of gold(iii), the probe displays ratiometric fluorescence behaviour clearly observable to the naked eye under both visible and UV light.
RESUMEN
An ESIPT-based fluorescent dye, 3-hydroxyflavone, is chemically masked with an electrophilic cyanate motif in order to construct a fluorescent probe for cellular sulfur species. This novel probe structure, displays an extremely fast, highly sensitive and selective "turn-on" type fluorescent response toward H2S. We have also documented its utility for imaging of H2S in the living cells.
Asunto(s)
Cianatos/química , Flavonoides/química , Colorantes Fluorescentes/química , Sulfuro de Hidrógeno/análisis , Sulfuro de Hidrógeno/química , Línea Celular Tumoral , Supervivencia Celular , Fluorescencia , Humanos , Estructura Molecular , Espectrometría de FluorescenciaRESUMEN
A fluorescent probe that displays a ratiometric fluorescence response towards gold and mercury ions has been devised. Emitting at a relatively longer wavelength, the conjugated form of the fluorescent dye transforms in the presence of the gold or mercury ions into a new dye, the molecular structure of which lacks the conjugation and consequently emits at a distinctly shorter wavelength.
RESUMEN
A BODIPY-based fluorescent probe bearing a pyridyl hydrazone motif responds selectively to Au(III) ions through an irreversible C=N bond hydrolysis reaction.
Asunto(s)
Compuestos de Boro/química , Colorantes Fluorescentes/química , Oro/análisis , Espectrometría de Fluorescencia , Espectrofotometría UltravioletaRESUMEN
We described the design and synthesis of a molecular sensor based on a rhodamine/BODIPY platform that displayed differential fluorescence responses towards Hg(2+) and Au(3+) and demonstrated its utility in intracellular ion imaging.
RESUMEN
A BODIPY-based fluorescent probe integrated with an aldoxime unit shows a remarkable fluorescence ''turn-on'' response to hypochlorous acid (HOCl). The oxidative dehydrogenation of BODIPY aldoxime by HOCl results in a distinct fluorescence enhancement as well as a change in color from red to orange.
Asunto(s)
Compuestos de Boro/química , Ácido Hipocloroso/análisis , Espectrometría de Fluorescencia , Línea Celular Tumoral , Colorantes Fluorescentes/química , Humanos , Microscopía Confocal , Oxidación-ReducciónRESUMEN
A rhodamine-based fluorescent probe, functionalized with an alkyne moiety, shows highly selective recognition towards Au(i) and Au(iii) ions over other metal ions. Probe is successfully employed for the determination of residual gold species in synthetic samples and monitoring the accumulation of gold ions in living cells.
Asunto(s)
Técnicas de Química Analítica/instrumentación , Oro/análisis , Oro/química , Rodaminas/química , Catálisis , Supervivencia Celular , Colorantes Fluorescentes/química , Células HCT116 , HumanosRESUMEN
The tandem hydroamination-annulation reaction of 4-pentyne-nitriles in the presence of amine nucleophiles and a cooperatively operating catalyst system, consisting of Ph(3)PAuCl and Zn(ClO(4))(2), provides an efficient route to 2-aminopyrroles. Two regioisomeric 2-aminopyrroles were formed in moderate to good yields.
RESUMEN
Acyl phosphonates with alpha-halo ketones in the presence of bases at room temperature afford cis- and trans-epoxyphosphonates in good chemical yields and high selectivities using different bases. The diastereoselectivity of this reaction is easily controlled by changing the base. Changing the base from Cs2CO3 to DBU changed the diastereomeric ratio (trans/cis) from 3/2 to 9/1. Moreover, the treatment of the trans isomer with DBU showed a complete conversion to the corresponding cis isomer.
Asunto(s)
Bromo/química , Compuestos Epoxi/síntesis química , Cetonas/química , Organofosfonatos/química , Acetonitrilos/química , Compuestos Epoxi/química , Estereoisomerismo , Especificidad por SustratoRESUMEN
Certain Cu(I) and Cu(II) salts are able to mediate the dimerization of arylboronic acids in DMF. They provide the corresponding symmetrical biaryls in moderate to very good yields. It is possible to run the reaction catalytically under an oxygen atmosphere without a significant loss of yields.
RESUMEN
The efficient generation of aryl radicals from arylboronic acids by manganese(III) acetate is described. In aromatic solvents, in situ generated aryl radicals afford the corresponding biaryls in very good yields. This method works selectively, and yields are better than those from similar, previously described methods. Arylboronic acids carrying sensitive functional groups also work efficiently.