RESUMEN

In 2012, the Centers for Disease Control and Prevention (CDC) established the Achieving Public Health Impact through Research (APHIR) contract mechanism. APHIR provides CDC's Centers, Institute, and Offices (CIOs) a mechanism that supports multiyear, high impact public health research. Awarded projects supported research on a wide range of topics (e.g., cancer surveillance, HIV education programs, development of biological assays, and evaluation of traumatic brain injury prevention programs) and achieved diverse outcomes (e.g., contribution to the body of knowledge in their field, changes in practice and health service delivery, and capacity building). This article describes how existing impact frameworks and a variety of methods and tools (key informant interviews, online survey, bibliometric analysis, Altmetric and document reviews) were used to identify the outcomes achieved by awarded projects. The approach discussed in this paper can be used to evaluate projects that involve a diversity of activities and outcomes.

Asunto(s)

Creación de Capacidad , Salud Pública , Centers for Disease Control and Prevention, U.S. , Humanos , Evaluación de Programas y Proyectos de Salud , Estados Unidos

RESUMEN

Oliveros virus (OLV) is an arenavirus hosted by the sigmodontine rodent, Necromys benefactus, in central Argentina. We report a 3-year longitudinal field study of the dynamics of OLV infection in host populations from 15 localities in two provinces on the central Argentine pampa. There was an overall 3-year period immunofluorescent antibody prevalence of 25% in the host population, and infected hosts were found throughout the study area. Spill-over infection into common sympatric species was rare. Infection dynamics exhibited many of the patterns seen for other rodent-borne arenaviruses and hantaviruses, but had some unique characteristics. Host population density was highest in autumn and lowest in spring, while antibody prevalence was highest in spring and lowest in autumn. Virus transmission was horizontal: infection was strongly associated with age, reaching 45% prevalence in the oldest individuals, and prevalence of infection was equal among male and female hosts. Infection may have been associated with scars, which were also approximately equally distributed among male and female Necromys.

Asunto(s)

Infecciones por Arenaviridae/veterinaria , Arenavirus/fisiología , Enfermedades de los Roedores/epidemiología , Enfermedades de los Roedores/virología , Sigmodontinae/virología , Factores de Edad , Animales , Anticuerpos Antivirales/sangre , Antígenos Virales/metabolismo , Infecciones por Arenaviridae/epidemiología , Infecciones por Arenaviridae/virología , Arenavirus/aislamiento & purificación , Argentina/epidemiología , Cicatriz/epidemiología , Cicatriz/veterinaria , Femenino , Incidencia , Estudios Longitudinales , Masculino , Densidad de Población , Prevalencia , Estaciones del Año

RESUMEN

Prevalence of antibody reactive with Sin Nombre hantavirus (SNV) was evaluated from rodents captured over 31 months (March 1988 to September 1990) from six mark-recapture grids on the central Argentine Pampa. The most frequently infected rodents were: Akodon azarae (31/459), Necromys benefactus (8/141), and Oligoryzomys flavescens (10/281), which are known hosts of Pergamino, Maciel, and Lechiguanas hantaviruses, respectively. Relative population density and antibody prevalence varied seasonally and from year to year, population densities were highest in fall and prevalences were highest in spring. A positive association between antibody prevalence and body weight corroborated findings from other studies suggesting that hantaviruses are maintained in reservoir populations by horizontal transmission. In two of three host species, transmission was more frequent among male than among female mice. We found no evidence for a detrimental effect of hantavirus infection on host body weight, growth, longevity, movement, or reproductive preparedness. This analysis, based on cryopreserved specimens, represents the earliest conducted longitudinal, mark-recapture study of the dynamics of infection of autochthonous American hantaviruses in their sigmodontine host populations.

Asunto(s)

Anticuerpos Antivirales/sangre , Reservorios de Enfermedades/veterinaria , Infecciones por Hantavirus/veterinaria , Orthohantavirus/inmunología , Enfermedades de los Roedores/epidemiología , Animales , Argentina/epidemiología , Peso Corporal , Femenino , Geografía , Orthohantavirus/aislamiento & purificación , Infecciones por Hantavirus/epidemiología , Infecciones por Hantavirus/transmisión , Síndrome Pulmonar por Hantavirus/epidemiología , Síndrome Pulmonar por Hantavirus/transmisión , Síndrome Pulmonar por Hantavirus/veterinaria , Humanos , Estudios Longitudinales , Masculino , Densidad de Población , Enfermedades de los Roedores/transmisión , Roedores , Estaciones del Año , Estudios Seroepidemiológicos , Factores Sexuales , Virus Sin Nombre/inmunología , Virus Sin Nombre/aislamiento & purificación , Especificidad de la Especie

RESUMEN

Gray squirrels, Sciurus carolinensis, were livetrapped in 2 different habitat types, woodland (67 squirrels) and parkland (53 squirrels), in southeastern Georgia. Ectoparasites were recovered from anesthetized squirrels and compared between hosts from the 2 habitats. Because of the absence of low vegetation in parkland habitats, it was hypothesized that the ectoparasite fauna, especially ticks and chiggers, would be more diverse on woodland squirrels. The results were generally in agreement with this hypothesis. Seventeen species of ectoparasites were recovered from woodland squirrels, compared with 6 species from parkland squirrels. Five species of ticks and 3 species of chiggers parasitized the woodland squirrels compared with no ticks or chiggers on the parkland squirrels. Significantly higher infestation prevalences were recorded on woodland compared with parkland squirrels for the flea Orchopeas howardi, the tick Amblyomma americanum, and the mesostigmatid mite Androlaelaps fahrenholzi. The mean intensity for O. howardi also was significantly higher on woodland than on parkland squirrels. Because a new strain of Bartonella sp. was isolated recently from S. carolinensis in Georgia, selected ectoparasites from this study were screened for bartonellae by polymerase chain reaction (PCR). Some of the fleas and lice, but none of the mites tested, were PCR positive, suggesting that fleas, or lice, or both, might be vectors of bartonellae between squirrels. Six distinct strains of Bartonella sp. were detected, 2 in fleas and 4 in lice.

Asunto(s)

Vectores Artrópodos/microbiología , Bartonellaceae/aislamiento & purificación , Infestaciones Ectoparasitarias/veterinaria , Enfermedades de los Roedores/parasitología , Sciuridae/parasitología , Animales , Anoplura/genética , Anoplura/microbiología , Vectores Artrópodos/genética , Bartonellaceae/genética , Infecciones por Bartonellaceae/transmisión , Infecciones por Bartonellaceae/veterinaria , ADN/química , Infestaciones Ectoparasitarias/epidemiología , Infestaciones Ectoparasitarias/parasitología , Ambiente , Georgia/epidemiología , Ácaros/genética , Ácaros/microbiología , Reacción en Cadena de la Polimerasa/veterinaria , Prevalencia , Enfermedades de los Roedores/epidemiología , Siphonaptera/genética , Siphonaptera/microbiología , Garrapatas/genética , Garrapatas/microbiología , Trombiculidae/genética , Trombiculidae/microbiología

RESUMEN

We conducted a population-based study to assess demographic and risk-factor correlates for the most frequently occurring Mycobacterium tuberculosis genotypes from tuberculosis (TB) patients. The study included all incident, culture-positive TB patients from seven sentinel surveillance sites in the United States from 1996 to 2000. M. tuberculosis isolates were genotyped by IS6110-based restriction fragment length polymorphism and spoligotyping. Genotyping was available for 90% of 11923 TB patients. Overall, 48% of cases had isolates that matched those from another patient, including 64% of U.S.-born and 35% of foreign-born patients. By logistic regression analysis, risk factors for clustering of genotypes were being male, U.S.-born, black, homeless, and infected with HIV; having pulmonary disease with cavitations on chest radiograph and a sputum smear with acid-fast bacilli; and excessive drug or alcohol use. Molecular characterization of TB isolates permitted risk correlates for clusters and specific genotypes to be described and provided information regarding cluster dynamics over time.

Asunto(s)

Mycobacterium tuberculosis/genética , Vigilancia de Guardia , Tuberculosis/epidemiología , Tuberculosis/microbiología , Adolescente , Adulto , Anciano , Antituberculosos/farmacología , Antituberculosos/uso terapéutico , Niño , Preescolar , Análisis por Conglomerados , ADN Bacteriano/genética , Farmacorresistencia Bacteriana Múltiple , Femenino , Genotipo , Humanos , Lactante , Estudios Longitudinales , Masculino , Persona de Mediana Edad , Epidemiología Molecular , Mycobacterium tuberculosis/clasificación , Mycobacterium tuberculosis/efectos de los fármacos , Mycobacterium tuberculosis/aislamiento & purificación , Polimorfismo de Longitud del Fragmento de Restricción , Grupos Raciales , Factores de Riesgo , Factores de Tiempo , Tuberculosis/tratamiento farmacológico , Estados Unidos

RESUMEN

To better understand the molecular epidemiology of tuberculosis (TB) transmission for culture-confirmed patients <5 years of age, data were analyzed from a population-based study conducted in seven U.S. sites from 1996 to 2000. Mycobacterium tuberculosis isolates were genotyped with IS6110-based restriction fragment length polymorphism analysis and spoligotyping. Case-patient data were obtained from the Centers for Disease Control and Prevention s national tuberculosis registry and health department records. Routine public health investigations conducted by local health departments identified suspected source patients for 57 (51%) of 111 culture-confirmed patients <5 years of age. For 8 (15%) of 52 culture-confirmed patients <5 years of age and their suspected source patients with complete genotyping results, genotypes suggested infection with different TB strains. Potential differences between sources for patients <5 years of age and source patients that transmitted TB to adolescent and adult patients were identified.

Asunto(s)

Trazado de Contacto , Mycobacterium tuberculosis/genética , Tuberculosis/transmisión , Adolescente , Adulto , Antituberculosos/farmacología , Niño , Preescolar , Dermatoglifia del ADN , ADN Bacteriano/análisis , Bases de Datos Factuales , Farmacorresistencia Bacteriana Múltiple , Femenino , Humanos , Lactante , Masculino , Epidemiología Molecular , Mycobacterium tuberculosis/clasificación , Mycobacterium tuberculosis/efectos de los fármacos , Polimorfismo de Longitud del Fragmento de Restricción , Factores de Riesgo , Vigilancia de Guardia , Tuberculosis/epidemiología , Tuberculosis/microbiología , Tuberculosis/prevención & control , Estados Unidos/epidemiología

RESUMEN

DNA fingerprinting was used to evaluate epidemiologically linked case pairs found during routine tuberculosis (TB) contact investigations in seven sentinel sites from 1996 to 2000. Transmission was confirmed when the DNA fingerprints of source and secondary cases matched. Of 538 case pairs identified, 156 (29%) did not have matching fingerprints. Case pairs from the same household were no more likely to have confirmed transmission than those linked elsewhere. Case pairs with unconfirmed transmission were more likely to include a smear-negative source case (odds ratio [OR] 2.0) or a foreign-born secondary case (OR 3.4) and less likely to include a secondary case <15 years old (OR 0.3). Our study suggests that contact investigations should focus not only on the household but also on all settings frequented by an index case. Foreign-born persons with TB may have been infected previously in high-prevalence countries; screening and preventive measures recommended by the Institute of Medicine could prevent TB reactivation in these cases.

Asunto(s)

Dermatoglifia del ADN , Mycobacterium tuberculosis/clasificación , Mycobacterium tuberculosis/genética , Tuberculosis/epidemiología , Tuberculosis/microbiología , Adolescente , Adulto , Anciano , Niño , Trazado de Contacto , ADN Bacteriano/análisis , Femenino , Humanos , Masculino , Persona de Mediana Edad , Vigilancia de Guardia , Tuberculosis/transmisión , Estados Unidos/epidemiología

RESUMEN

Ionic concentration and annual deposition of NO -3 -N, NH +4 -N, Ca2+, and Mg2+ from bulk precipitation and dry atmospheric deposition were studied for one year in southern California. Data were collected from an inland chaparral site at 1,300 m elevation, 75 km from the coast. The annual depositions of NO -3 -N and NH +4 -N amounted to 96.3 and 56.0 mg m-2 ground area yr-1, respectively. The corresponding values for calcium and magnesium ions were 207.4 and 57.4 mg m-2 yr-1. The average pH of rainwater was 3.74 (range 3.37 to 4.75), thus documenting acid rain for an inland site in California, distant from urban sources of air contamination. An estimate of nitrogen gains and losses indicated that the time between recurrent chaparral fires should be about 60 years in order to maintain a balanced nitrogen budget.