RESUMEN
Capacitively coupled contactless conductivity detection (C4D) has emerged as influential to detect analytes that do not have chromogenic or fluorogenic functional group. Since our last review several new capillary electrophoresis (CE) methods coupled with (CE-C4D) have been communicated. The aim of this review is to give an update of the almost all the new applications of CE-C4D in the field of pharmaceutical, food and biomedical analysis covering the period from 2017 to April 2020. The utilization of CE with C4D in the areas of pharmaceutical, food and biomedical analysis is presented. Finally, concluding remarks and outlooks are discussed.
Asunto(s)
Electroforesis Capilar , Conductividad Eléctrica , Electroforesis Capilar/métodos , Preparaciones FarmacéuticasRESUMEN
Three rapid, accurate, and ecofriendly processed spectrophotometric methods were validated for the concurrent quantification of remogliflozin (RGE) and vildagliptin (VGN) from formulations using water as dilution solvent. The three methods developed were based on the calculation of the peak height of the first derivative absorption spectra at zero-crossing points, the peak amplitude difference at selected wavelengths of the peak and valley of the ratio spectra, and the peak height of the ratio first derivative spectra. All three methods were validated adapting the ICH regulations. Both the analytes showed a worthy linearity in the concentration of 1 to 60 µg/mL and 2 to 90 µg/mL for VGN and RGE, respectively, with an exceptional regression coefficient (r2 ≥ 0.999). The developed methods demonstrated an excellent recovery (98.00% to 102%), a lower percent relative standard deviation, and a relative error (less than ±2%), confirming the specificity, precision, and accuracy of the proposed methods. In addition, validated spectrophotometric methods were commendably employed for the simultaneous determination of VGN and RGE from solutions prepared in the laboratory and the formulation. Hence, these methods can be utilized for the routine quality control study of the pharmaceutical preparations of VGN and RGE in pharmaceutical industries and laboratories. The ecofriendly nature of the anticipated spectrophotometric procedures was confirmed by the evaluation of the greenness profile by a semi-quantitative method and the quantitative and qualitative green analytical procedure index (GAPI) method.