RESUMEN
The discovery of Helicobacter hepaticus as a causal agent of hepatitis and hepatocellular carcinoma (HCC) in mice has stimulated interest in looking for Helicobacter species in human liver samples. In this study, we searched for association between H. pylori and HCV-related liver disease. Liver specimens were collected from eighty-five patients; they were divided into five different groups according to liver pathology (METAVIR system). Group I (the 1st control group) consisted of 16 patients with chronic hepatitis C without histological activity. Group II consisted of 25 patients with chronic active hepatitis C, Group III, 17 patients with HCV-related cirrhosis and Group IV, 16 patients with HCV-related cirrhosis and HCC. Group V (2nd control group) consisted of 11 patients suffering from gastro duodenal and gall bladder diseases but negative for HCV. All cases were tested by polymerase chain reaction on liver samples for the presence of H. pylori DNA Cag A gene. Routine biochemical, radiological and RT-PCR for HCV RNA were also performed for all cases. The positivity of H. pylori PCR CagA gene in liver tissue was directly proportional to the severity of liver pathology, this being 75%, 52.9% and 32% in groups IV, III and II, respectively, which was more significant than the 1st and 2nd control groups (P < 0.001). There was a significant difference between H. pylori PCR values when compared to METAVIR staging (F) in different groups (P = 0.001). Helicobacter pylori PCR (Cag A gene) was positive in about 28.2% cases of late fibrosis (F3 + F4) while positivity was (5.9%) in early fibrosis (F1 + F2) (P = 0.0001). There was significant difference between H. pylori PCR (Cag A gene) in liver tissue and METAVIR activity in different groups (P = 0.002) as most of H. pylori PCR-positive cases were METAVIR activity A1 and A2 (15.3% and 12.9%, respectively). There was no association between H. pylori PCR and quantitative HCV RNA (P = 0.531). Also there was no significant difference of Child-Pugh staging in the H. pylori PCR-positive group when compared to the negative group (P = 0.996). There may be an association between the presence of H. pylori (Cag A gene) in the liver and disease progression in HCV-related chronic hepatitis and cirrhosis with and without HCC.
Asunto(s)
Infecciones por Helicobacter/complicaciones , Helicobacter pylori/patogenicidad , Hepatitis C Crónica/complicaciones , Hepatitis C Crónica/patología , Cirrosis Hepática/complicaciones , Cirrosis Hepática/patología , Antígenos Bacterianos/genética , Proteínas Bacterianas/genética , Biopsia , ADN Bacteriano/genética , Progresión de la Enfermedad , Infecciones por Helicobacter/microbiología , Helicobacter pylori/aislamiento & purificación , Hepacivirus/aislamiento & purificación , Humanos , Hígado/microbiología , Hígado/patología , Reacción en Cadena de la Polimerasa , ARN Viral/genética , Índice de Severidad de la EnfermedadRESUMEN
Phytochemical investigation of air-dried powdered roots of Artemisia monosperma growing in Egypt afforded two new compounds; 6-hydroxy-7,8-dimethoxycoumarin (I) and 5-acetyl-2-[1'-(hydroxymethyl)ethyl]-2,3-dihydrobenzo[b]furan (IV), in addition to the known compounds; 6-hydroxy-5,7-dimethoxycoumarin (fraxinol) (II), 5-hydroxy-6,7-dimethoxycoumarin (tomentin) (III) and methyl-beta-D-fructofuranoside (V), obtained for the first time from the plant. Chemical structures of the isolated compounds were assigned based on different physical, chemical and spectroscopic techniques including UV, IR, MS, 1D- and 2D-NMR spectra. Furthermore, antimicrobial activity of different extracts of roots was carried out.
Asunto(s)
Artemisia/química , Artemisia/clasificación , Bacterias/efectos de los fármacos , Espectroscopía de Resonancia Magnética , Pruebas de Sensibilidad Microbiana , Extractos Vegetales/farmacología , Raíces de Plantas/química , Espectrometría de Masa por Ionización de Electrospray , Espectrofotometría Infrarroja , Espectrofotometría UltravioletaRESUMEN
Two new alkaloids, namely 8-hydroxylycorin-7-one and 2-deoxylycorine were isolated from Crinum bulbispermum along with the known compounds vittatine, 11-hydroxyvittatine and hippamine.
Asunto(s)
Alcaloides/química , Alcaloides de Amaryllidaceae/química , Crinum/química , Alcaloides/aislamiento & purificación , Alcaloides/toxicidad , Alcaloides de Amaryllidaceae/aislamiento & purificación , Alcaloides de Amaryllidaceae/toxicidad , Animales , Artemia , Cromatografía en Capa Delgada , Dosificación Letal Mediana , Espectroscopía de Resonancia Magnética , Espectrometría de Masas , Raíces de Plantas/química , Espectrofotometría UltravioletaAsunto(s)
Antiinfecciosos/farmacología , Aceites Volátiles/farmacología , Aceites de Plantas/farmacología , Antibacterianos , Antifúngicos/farmacología , Bacterias/efectos de los fármacos , Hongos/efectos de los fármacos , Pruebas de Sensibilidad Microbiana , Aceites Volátiles/análisis , Aceites de Plantas/análisis , Plantas Medicinales/químicaRESUMEN
Cell proliferation in carcinoma in the bilharzial bladder was studied in 92 patients in terms of the in vitro labeling index (LI), cell density (CD) and labeled cell density (LCD) using the in vitro 3H-Tdr technique. Cell proliferation was much greater in high than in low grade tumors and in deep than in superficial parts of the tumor, but was much less dependent on cell type; transitional cell cancer had the highest activity followed by squamous cell and adenocarcinoma. The probability of local recurrence after cystectomy decreased markedly when the LI exceeded 5.0%. The influence of the following three pre-operative radiotherapy regimens was studied: split-course (SC): the initial course consisted of 20 Gy in 10 treatments with a similar course was given after one week, hyper-fractionation using 17 treatments 0.6 Gy each on two successive days, this 2-day course of 20 Gy was repeated after one week, and concentrated irradiation consisting of two treatments, 6.0 Gy each with a gap of one week. Cystectomy was performed 14-20 days after treatment in all groups. Preoperative irradiation was generally associated with an increased probability of local control. The unfavorable influence of a high pretreatment LI was not noted after pre-operative irradiation. The CD was also reduced in proportion to the pretreatment LI. It is proposed that the response to irradiation was proportional to the initial proliferation activity and hence the prognostic significance of tumor grade and pretreatment LI was masked. Postirradiation tumor volume reduction was a strong predictor of treatment outcome. Concentrated irradiation was the least efficient pre-operative irradiation regimen and was associated with the least tumor volume reduction.