RESUMEN
Here, we present the draft genome assembly of Escherichia coli DBS1, which was originally isolated from a urine sample from a male patient with urinary tract infections in Rabat, Morocco.
RESUMEN
PURPOSE: Incidence of pediatric urolithiasis is decreasing in most developing countries where endemic bladder stones are less prevalent than in the past years. In parallel, stone composition has changed. Only few data are available in North Africa, except for Tunisia. We report stone composition in the Moroccan pediatric population. MATERIAL AND METHODS: Composition of 432 stones from children (302 boys, 130 girls) was determined by infrared spectroscopy. The samples were collected during the period 1999-2016. Stone morphology, which is an important aspect for etiology was determined by examination of each stone under a stereomicroscope. Stone composition was compared to patients' age and gender. RESULTS: The global male-to-female ratio was 2.32. Regarding stone composition, calcium oxalate was the main component in 51.6% of the stones, followed by struvite (18.1%), ammonium urate (9.5%) and carbapatite (9%). Significant differences were found between males and females: calcium oxalate accounted for 72.3% of stones in girls and 42.7% in boys (P<10-6); conversely, struvite was more frequent in boys than in girls (22.2 vs 8.5%, P<10-4). The same was found for calcium phosphate stones (11.9% in boys; 4.6% in girls, P<0.05). Stone morphology helped us for detecting several pathological conditions: type Ic whewellite stones, as a marker for primary hyperoxaluria, was found in 17.6% of stones and type IIId ammonium urate stones, suggestive for infectious diarrhea, was identified in 20.1% of all stones. CONCLUSION: This is the largest series of pediatric stones in Morocco studied by infrared analysis and morphological examination. LEVEL OF EVIDENCE: 3.
Asunto(s)
Oxalato de Calcio/química , Espectrofotometría Infrarroja/métodos , Cálculos Urinarios/química , Urolitiasis/epidemiología , Adolescente , Factores de Edad , Apatitas/química , Niño , Preescolar , Femenino , Humanos , Incidencia , Lactante , Masculino , Marruecos/epidemiología , Factores Sexuales , Estruvita/química , Cálculos Urinarios/epidemiologíaRESUMEN
Here, we report the draft genome sequences of methicillin-susceptible Staphylococcus captis pulsotype NCRS-C (CR02 strain) and multiresistant Staphylococcus captis pulsotype NCRS-A (CR07 strain).
RESUMEN
Staphylococcus capitis pulsotype NRCS-A was previously reported as a frequent cause of late-onset sepsis in neonatal intensive care units (NICUs) worldwide. Here, we report the whole-genome shotgun sequences of four S. capitis pulsotype NCRS-A strains, CR03, CR04, CR05, and CR09, isolated from Belgium, Australia, the United Kingdom, and France, respectively.
RESUMEN
Staphylococcus capitis is a coagulase-negative staphylococcus (CoNS) commonly found in the human microflora. Recently, a clonal population of Staphylococcus capitis (denominated NRCS-A) was found to be a major cause of late-onset sepsis (LOS) in several neonatal intensive care units in France. Here, we report the complete genome sequence and annotation of the prototype Staphylococcus capitis NCRS-A strain CR01. The 2,504,472 bp long genome (1 chromosome and no plasmids) exhibits a G+C content of 32.81%, and contains 2,468 protein-coding and 59 tRNA genes and 4 rRNA genes.
RESUMEN
Multiresistant Staphylococcus capitis pulsotype NRCS-A has been reported to be a major pathogen causing nosocomial bacteremia in preterm infants. We report that the NRCS-A strain CR01 harbors a novel 60.9-kb composite staphylococcal cassette chromosome mec (SCCmec) element, composed of an SCCmec with strong homologies to Staphylococcus aureus ST398 SCCmec and of an SCCcad/ars/cop harboring resistance genes for cadmium, arsenic, and copper. Whole-genome-based comparisons of published S. capitis strains suggest that strain CR01 acquired the two elements independently.
Asunto(s)
Staphylococcus aureus Resistente a Meticilina/genética , Sepsis/microbiología , Arsénico/farmacología , Cadmio/farmacología , Cromosomas Bacterianos/genética , Cobre/farmacología , Femenino , Humanos , Recién Nacido , MasculinoRESUMEN
INTRODUCTION: cystine stones represent 1% of urinary calculi in adults and 10% in children and are especially recurrent and resistant to ESWL. Medical therapy is often efficient but often poorly tolerated. In Morocco, various plants, Herniaria hirsuta, Opuntia ficus-indica, Zea mays and Ammi visnaga are proposed against nephrolithiasis. We assessed the effect of plant extracts on the disolution of cystine stones in vitro. MATERIAL AND METHOD: an extract of each plant was prepared by infusion of two grams of powdered plants during 30 minutes in 100ml of a boiled NaCl 9 g/L aqueous solution. Each extract was then filtered and thereafter set in a flask containing a cystine stone. The medium was maintained under stirring during 8 weeks. NaCl 9 g/L solution and sodium citrate 3 mmol/L solution were used as controls. At the end of each two weeks period, the stone was removed from the experimental medium and weighted after a 16h drying period at 40°C. RESULTS: after 8 weeks of experiment, stone dissolution was complete for all herbal extracts, and was earlier in the presence of Zea mays (4 weeks) or of Ammi visnaga (6 weeks) by comparison to only 18 ± 8 and 20 ± 1.5 % for citrate and NaCl solutions, respectively. CONCLUSION: the studied herbal extracts were efficient for dissolving cystine stones, probably resulting from the formation of complexes between cystine and polyhydroxylated molecules present in the extracts. These results, to be confirmed in vivo, underline the potential interest of the plant extracts to treat cystine stones.