RESUMEN
Optical frequency comb-referenced measurements of self pressure-broadened line profiles of the R(8) to R(13) lines in the ν1 + ν3 combination band of acetylene near 1.52 µm are reported. The analysis of the data found no evidence for a previously reported [Iwakuni et al., Phys. Rev. Lett. 117(14), 143902 (2016)] systematic alternation in self pressure-broadened line widths with the nuclear spin state of the molecule. The present work brought out the need for the use of an accurate line profile model and careful accounting for weak background absorptions due to hot band and lower abundance isotopomer lines. The data were adequately fit using the quadratic speed-dependent Voigt profile model, neglecting the small speed-dependent shift. Parameters describing the most probable and speed-dependent pressure-broadening, most probable shift, and the line strength were determined for each line. Detailed modeling of the results of Iwakuni et al. showed that their neglect of collisional narrowing due to the speed-dependent broadening term combined with the strongly absorbing data recorded and analyzed in transmission mode were the reasons for their results.
RESUMEN
Cancer stem cells (CSCs) have been implicated in the initiation and maintenance of tumour growth as well as metastasis. Recent reports link stemness to epithelial-mesenchymal transition (EMT) in cancer. However, there is still little knowledge about the molecular markers of those events. In silico analysis of RNA profiles of 36 pancreatic ductal adenocarcinomas (PDAC) reveals an association of the expression of CD95 with EMT and stemness that was validated in CSCs isolated from PDAC surgical specimens. CD95 expression was also higher in metastatic pancreatic cells than in primary PDAC. Pharmacological inhibition of CD95 activity reduced PDAC growth and metastasis in CSC-derived xenografts and in a murine syngeneic model. On the mechanistic level, Sck was identified as a novel molecule indispensable for CD95's induction of cell cycle progression. This study uncovers CD95 as a marker of EMT and stemness in PDAC. It also addresses the molecular mechanism by which CD95 drives tumour growth and opens tantalizing therapeutic possibilities in PDAC.
Asunto(s)
Carcinoma Ductal Pancreático/fisiopatología , Metástasis de la Neoplasia , Neoplasias Pancreáticas/fisiopatología , Proteínas Adaptadoras de la Señalización Shc/fisiología , Receptor fas/fisiología , Animales , Biomarcadores de Tumor , Carcinoma Ductal Pancreático/metabolismo , Transición Epitelial-Mesenquimal , Femenino , Humanos , Ratones , Neoplasias Pancreáticas/metabolismo , Transducción de Señal , Proteína Transformadora 2 que Contiene Dominios de Homología 2 de Src , Ensayos Antitumor por Modelo de XenoinjertoRESUMEN
In addition to the classical genomic steroid actions on modulation of transcription and protein synthesis, rapid, nongenomic effects have been described for various steroids. These effects on cellular signaling and function are supposed to be transmitted by membrane binding sites unrelated to the classical intracellular receptors. Recently, a high affinity progesterone membrane binding protein (mPR) has been characterized in porcine liver membranes. In the present study, amino acid residues that are essential for progesterone binding to porcine liver microsomal mPR have been identified by the use of protein modifying reagents. Among all reagents tested, agents with specificity for carboxyl groups, methionine and tryptophan such as N,N'-dicyclohexylcarbodiimide, chloramine T and N-bromosuccinimide induced a reduction in [3H]progesterone binding. To evaluate the presence of essential disulfide bridges, porcine liver microsomes were incubated with the disulfide reducing agent dithiothreitol (DTT) and [3H]progesterone binding was measured. This treatment also resulted in a reduction of binding activity with an IC50 of 20 mM for DTT. Western-blotting analysis in the presence or absence of the reducing agent suggested that mPR--in its binding state--consists of at least two identical subunits with an apparent molecular mass of 28 kDa which are linked by a disulfide bridge. In conclusion, in the present study evidence for an involvement of carboxyl-, tryptophan- and methionine residues in [3H]progesterone binding to porcine liver microsomes is given. In addition, it is shown that mPR can form disulfide-linked homodimers.
Asunto(s)
Bromosuccinimida/farmacología , Cloraminas/farmacología , Diciclohexilcarbodiimida/farmacología , Ditiotreitol/farmacología , Proteínas de la Membrana/metabolismo , Microsomas Hepáticos/metabolismo , Progesterona/metabolismo , Receptores de Progesterona/metabolismo , Compuestos de Tosilo/farmacología , Animales , Sitios de Unión , Western Blotting , Dimerización , Electroforesis en Gel de Poliacrilamida , Ligandos , Unión Proteica , Sensibilidad y Especificidad , PorcinosRESUMEN
OBJECTIVE: This article compares the accuracy of CT with that of MR imaging in staging of malignant pleural mesothelioma. SUBJECTS AND METHODS: Ninety-five patients were enrolled in a prospective staging protocol based on the International Mesothelioma Interest Group staging system. Sixty-five patients underwent CT and MR imaging and a surgical procedure (excluding percutaneous needle biopsy) to stage and resect the tumor. Receiver operating characteristic analyses were performed. CT and MR scans were interpreted independently by observers who were unaware of the results of the other imaging study; these imaging findings were compared with the results of surgery and pathologic examination. RESULTS: The areas under the receiver operating characteristic curves for eight of 10 features revealed by imaging showed no statistically significant differences between CT and MR imaging. However, MR imaging was superior to CT in revealing invasion of the diaphragm (A(z) = .55 for CT versus .82 for MR imaging) and in revealing invasion of endothoracic fascia or solitary resectable foci of chest wall invasion (A(z) = .46 for CT; A(z) = .69 for MR imaging). Several anatomic regions could not be evaluated because positive findings at surgery were rare. CONCLUSION: CT and MR imaging are of nearly equivalent diagnostic accuracy in staging malignant pleural mesothelioma. MR imaging is superior to CT in revealing solitary foci of chest wall invasion and endothoracic fascia involvement and in showing diaphragmatic muscle invasion; however, this advantage does not affect surgical treatment. For cost reasons, CT should be considered the standard diagnostic study before therapy.
Asunto(s)
Imagen por Resonancia Magnética , Mesotelioma/diagnóstico , Neoplasias Pleurales/diagnóstico , Tomografía Computarizada por Rayos X , Adulto , Anciano , Femenino , Humanos , Masculino , Mesotelioma/diagnóstico por imagen , Persona de Mediana Edad , Estadificación de Neoplasias , Neoplasias Pleurales/diagnóstico por imagen , Estudios Prospectivos , Curva ROCRESUMEN
A new derivative of progesterone was synthesized for photoaffinity labelling of specific binding sites in porcine liver microsomes. Using progesterone-3-(O-carboxymethyl)-oximino-(3-125I-iodo-4-azidosa licylamidobutylamine) as a photoactivatable radioligand, selective covalent labelling of proteins was detected in porcine liver microsomes at apparent molecular weights of 90-100 kDa and 60-65 kDa by SDS-PAGE and subsequent radioautography. These proteins showed different ligand specifity for various steroids tested. On blue native polyacrylamide-gels three selectively labelled proteins were found corresponding to apparent molecular weights of approximately 310 kDa, approximately 215 kDa and approximately 75 kDa, respectively. Using two-dimensional electrophoresis for the analysis of these protein complexes, the 215 kDa-band could be correlated to the 90-100 kDa-band, while the 75 kDa-band may correspond to the 60-65 kDa-band at one-dimensional SDS-PAGE, respectively. One or more of these proteins may be involved in the rapid progesterone-induced increase of intracellular Ca2+ described previously in cultured hepatic cells.
Asunto(s)
Sitios de Unión , Proteínas de la Membrana/metabolismo , Microsomas Hepáticos/metabolismo , Progesterona/metabolismo , Marcadores de Afinidad/metabolismo , Animales , Técnicas de Cultivo de Célula , Radioisótopos de Yodo/metabolismo , Ligandos , Progesterona/análogos & derivados , Esteroides/metabolismo , PorcinosRESUMEN
A full-length cDNA clone for a progesterone membrane binding protein from porcine vascular smooth muscle cells was isolated and the complete nucleotide sequence determined. The cDNA encodes a protein of 194 amino acids with a transmembrane segment. This protein is likely to represent the first steroid membrane receptor or a part of it for which sequence information is available.
Asunto(s)
Músculo Liso Vascular/química , Receptores de Progesterona/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Clonación Molecular , Secuencia Conservada , ADN Complementario/genética , Datos de Secuencia Molecular , Unión Proteica , Conformación Proteica , ARN Mensajero/genética , Receptores de Progesterona/metabolismo , Análisis de Secuencia de ADN , PorcinosRESUMEN
New derivatives of progesterone and aldosterone were synthesized and functionally tested with commercially available antibodies. The covalent labelling of antibodies specific for aldosterone and progesterone was detected by SDS/PAGE analysis and subsequent autoradiography after using 3-(O-carboxymethyl)-oximino-(3-[125I]iodo-4-azidosalicylamidobu tylamine) derivatives of aldosterone and progesterone, respectively, as photoactivatable radioligands. Labelling was not observed in the presence of an excess of the unlabelled steroid. Aldosterone was labelled with biotin and used as a tracer in a time-resolved fluorescence immunoassay. The nonradioactive tracer is highly selective for its antibody-binding site, with almost no detectable cross-reactivity for other steroids. Biotin-labelled progesterone was immobilized by avidin-agarose and used for affinity chromatography. This yielded a more than 20-fold enrichment of an anti-progesterone polyclonal antibody. These results demonstrate that derivatives of steroids are particularly useful for the development of nonradioactive assays for the determination of natural steroids and may be also useful for the detection of specific binding sites in biological material such as plasma membranes.
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Aldosterona/análogos & derivados , Progesterona/análogos & derivados , Marcadores de Afinidad , Aldosterona/química , Aldosterona/inmunología , Animales , Anticuerpos , Biotina , Bovinos , Cromatografía de Afinidad , Técnica del Anticuerpo Fluorescente , Ligandos , Fotoquímica , Progesterona/química , Progesterona/inmunología , Congéneres de la Progesterona/química , Congéneres de la Progesterona/inmunología , Albúmina Sérica BovinaRESUMEN
Increasing evidence has accumulated for rapid nongenomic steroid actions in various cell systems and, more recently, for rapid aldosterone effects on the Na(+)-H+ antiport in human mononuclear leukocytes. The aim of the present study was to demonstrate a rapid, nongenomic aldosterone action in rat vascular smooth muscle cells as a key effector cell in cardiovascular regulation. Basal 22Na+ influx in quiescent vascular smooth muscle cells was 22.1 +/- 1.9 nmol/mg protein per minute (mean +/- SEM, n = 9). Aldosterone (1 nmol/L) stimulated influx to 28.6 +/- 1.5 nmol/mg protein per minute after 4 minutes (n = 9, P < .05), with a half-maximal effect between 0.1 and 0.5 nmol/L; the effects were inhibited by ethylisopropylamiloride, the specific inhibitor of the Na(+)-H+ exchanger, demonstrating the involvement of this transport system in rapid effects of aldosterone. Hydrocortisone (1 mumol/L) was ineffective, and fludrocortisone and deoxycorticosterone increased influx with half-maximal effects at approximately 0.5 nmol/L. Canrenone, a classic antagonist of aldosterone action, did not inhibit stimulation by aldosterone at a 1000-fold excess concentration. Aldosterone significantly stimulated intracellular inositol 1,4,5-trisphosphate levels (P < .05) after 30 seconds; the inhibitors of phospholipase C, neomycin and U-73122, inhibited aldosterone-stimulated Na+ influx and increase of intracellular inositol 1,4,5-trisphosphate. The rapid stimulation of sodium transport in vascular smooth muscle cells and the pharmacological characteristics of this effect are clearly incompatible with the classic, genomic pathway of steroid action and represent further evidence for nongenomic effects of aldosterone.
Asunto(s)
Aldosterona/farmacología , Músculo Liso Vascular/metabolismo , Sodio/metabolismo , Amilorida/análogos & derivados , Amilorida/farmacología , Animales , Células Cultivadas , Relación Dosis-Respuesta a Droga , Inositol 1,4,5-Trifosfato/metabolismo , Transporte Iónico/efectos de los fármacos , Leucocitos Mononucleares/metabolismo , Masculino , Ratas , Ratas Sprague-Dawley , Intercambiadores de Sodio-Hidrógeno/efectos de los fármacosRESUMEN
Fast in vitro effects of aldosterone on the Na+/H(+)-exchanger, inositoltrisphosphate generation and corresponding specific binding to plasma membranes at Kd-values of approximately 0.1 nM have been found in human mononuclear leukocytes and vascular smooth muscle cells. The novel aldosterone membrane receptor was analyzed on SDS-PAGE after labeling of microsomal membranes from human mononuclear leukocytes with a [125I]-aldosterone-derivative by use of BASED as a photoactivatable crosslinker. Binding of 1 nM [125I]-aldosterone was found at a molecular weight of approximately 50 kDa which was absent with 1 microM cold aldosterone, but not cortisol in the binding media. This aldosterone-selectivity is typical and discriminatory for the new aldosterone membrane receptor. Solubilization of the receptor protein from membranes by high salt concentrations (1 M NaCl, 1 mM EDTA) was not achieved. It, thus, appears as an integral membrane protein. Dithiothreitol, a sulfhydryl agent, does not reduce specific aldosterone binding indicating the absence of SH-groups in the binding domain or sensitive structures of the receptors. The results are the first to characterize the novel membrane receptor for aldosterone with regard to molecular weight and basic properties. These findings and other related results are reviewed here.
Asunto(s)
Aldosterona/metabolismo , Leucocitos Mononucleares/química , Receptores de Mineralocorticoides/aislamiento & purificación , Ditiotreitol/farmacología , Electroforesis en Gel de Poliacrilamida , Humanos , Hidrocortisona/farmacología , Inositol 1,4,5-Trifosfato/metabolismo , Cinética , Masculino , Peso Molecular , Fotoquímica , Receptores de Mineralocorticoides/clasificación , Receptores de Mineralocorticoides/metabolismo , Intercambiadores de Sodio-Hidrógeno/metabolismoRESUMEN
Rapid, nongenomic in vitro effects of aldosterone on intracellular electrolytes, cell volume and the sodium-proton antiporter have been found in human mononuclear leukocytes (HML), as have related membrane receptors. In the present study, binding of 125I-labeled aldosterone to plasma membrane preparations from pig kidneys was studied, since nongenomic in vitro effects of aldosterone have also been described in cultured kidney cells. In this preparation, binding of aldosterone shares important features with both functional and binding data in HML. These include a very low apparent Ki of approximately 0.1 nM for aldosterone, a high turnover rate and binding selectivity for aldosterone and fludrocortisone. Desoxycorticosterone acetate and corticosterone show intermediate affinity, with apparent Ki values of approximately 1 and 100 nM, with hydrocortisone even less active. Thus binding of aldosterone to kidney plasma membranes is compatible with the major features of its nongenomic renal effects.
Asunto(s)
Membrana Celular/metabolismo , Riñón/metabolismo , Receptores de Mineralocorticoides/metabolismo , Aldosterona/análogos & derivados , Aldosterona/metabolismo , Animales , Corticosterona/metabolismo , Desoxicorticosterona/metabolismo , Fludrocortisona/metabolismo , Histamina/análogos & derivados , Histamina/metabolismo , Radioisótopos de Yodo , PorcinosRESUMEN
There is increasing evidence for rapid steroid action on electrolyte transport in human mononuclear leukocytes (HML). In HML, aldosterone stimulates the Na+/H+ antiporter within a few minutes. Because a variety of hormones and growth factors activate the Na+/H+ antiporter via protein kinase C and inositol phospholipids, a possible involvement of inositol-1,4,5-trisphosphate (IP3) in the rapid effects of aldosterone in HML was investigated. The stimulation of IP3 generation was started by the addition of aldosterone, concanavalin A, or other steroids. A significant increase in IP3 levels by aldosterone (1 nmol/L, P < 0.05) was found after 1 min, similar to that found after concanavalin A (25 micrograms/mL). Aldosterone caused a concentration-dependent elevation of IP3 levels, with an apparent EC50 of approximately 0.1 nmol/L. Fludrocortisone stimulated IP3 generation at similar concentrations, whereas a weaker IP3 stimulation by glucocorticoids (hydrocortisone, dexamethasone) occurred at micromolar concentrations only. Canrenone, a potent inhibitor of classical aldosterone action, was not effective up to a concentration of 100 nmol/L. These findings show kinetic and pharmacological similarities with both the functional data on Na+/H+ antiport stimulation by aldosterone and the studies of 125I-aldosterone binding to plasma membranes of HML. Thus, these data are the first to indicate an involvement of the phosphoinositide pathway in the rapid membrane effects of aldosterone.
Asunto(s)
Aldosterona/farmacología , Inositol 1,4,5-Trifosfato/biosíntesis , Leucocitos Mononucleares/efectos de los fármacos , Adulto , Calcio/metabolismo , Canrenona/farmacología , Concanavalina A/farmacología , Humanos , Hidrocortisona/farmacología , Leucocitos Mononucleares/metabolismo , Masculino , Intercambiadores de Sodio-Hidrógeno/efectos de los fármacos , Terpenos/farmacología , TapsigarginaRESUMEN
Non-genomic effects of aldosterone on the sodium-proton-antiport have been shown in human mononuclear leukocytes which could be related to a new aldosterone membrane receptor. In the present paper plasma membranes from human mononuclear leukocytes were covalently photolabeled with a [125I]-aldosterone derivative. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed significant aldosterone binding at a molecular weight of approximately 50000 Dalton which was absent with 1 microM cold aldosterone, but not cortisol in the binding media. The presence of the sulfhydryl agent dithiothreitol did not affect results suggesting the absence of disulfide bridges in the steroid binding domain of the receptor. These data are the first to define the molecular weight of the membrane receptor for aldosterone.
Asunto(s)
Aldosterona/metabolismo , Leucocitos Mononucleares/metabolismo , Receptores de Mineralocorticoides/metabolismo , Marcadores de Afinidad/metabolismo , Membrana Celular/metabolismo , Ditiotreitol/farmacología , Electroforesis en Gel de Poliacrilamida , Humanos , Radioisótopos de Yodo , Peso Molecular , Receptores de Mineralocorticoides/efectos de los fármacos , Receptores de Mineralocorticoides/aislamiento & purificaciónRESUMEN
Functional studies in extrarenal, non-epithelial cells such as smooth muscle cells and more recently circulating human lymphocytes have provided increasing evidence that aldosterone produces not only classical genomic effects, but also rapid, non-genomic effects on transmembrane electrolyte movements. These involve activation of the sodium/proton exchanger of the cell membrane at very low, physiological concentrations of aldosterone with an acute onset within 1-2 min. A second messenger cascade involved is the inositol 1,4,5-trisphosphate/calcium pathway which responds over the same rapid time course. Such changes clearly cannot be explained by genomic mechanisms, which are responsible for later effects than the membrane related rapid responses. The mechanisms underlying these rapid effects of aldosterone on electrolytes have been extensively studied in human lymphocytes, which thus may represent valuable tools in the delineation of the receptor-effector mechanisms involved. The unique characteristics of this new pathway for steroid action include its rapid time course, 10,000-fold selectivity for aldosterone over cortisol and the ineffectiveness of spironolactones, classical mineralocorticoid antagonists, as antagonists of the response.
Asunto(s)
Proteínas Portadoras/metabolismo , Mineralocorticoides/fisiología , Potasio/metabolismo , Receptores de Mineralocorticoides/fisiología , Sistemas de Mensajero Secundario/efectos de los fármacos , Sodio/metabolismo , Aldosterona/farmacología , Animales , Transporte Biológico , Tamaño de la Célula/efectos de los fármacos , Desoxicorticosterona/farmacología , Desoxicorticosterona/toxicidad , Perros , Humanos , Hidrocortisona/farmacología , Hipertensión/inducido químicamente , Túbulos Renales/efectos de los fármacos , Túbulos Renales/metabolismo , Leucocitos Mononucleares/efectos de los fármacos , Leucocitos Mononucleares/metabolismo , Antagonistas de Receptores de Mineralocorticoides , Mineralocorticoides/antagonistas & inhibidores , Mineralocorticoides/farmacología , Músculo Liso/efectos de los fármacos , Músculo Liso/metabolismo , Ratas , Intercambiadores de Sodio-Hidrógeno , ATPasa Intercambiadora de Sodio-Potasio/metabolismo , Espironolactona/farmacología , Porcinos , Factores de TiempoRESUMEN
Serum cortisol is one of the more frequently requested steroid hormone assays. Its use is important in evaluating diseases of the adrenal cortex and pituitary. We briefly review the biochemistry of cortisol synthesis, the pathophysiology resulting from adrenal and pituitary abnormalities and the more specific immunochemical procedures which have replaced colorimetric chemical assays for cortisol. We also report our results on the evaluation of the analytical performance of the non-isotopic homogeneous CEDIA Cortisol assay and compare the advantages of this assay to state-of-the-art immunoassays.
Asunto(s)
Hidrocortisona/sangre , Técnicas para Inmunoenzimas , Enfermedad de Addison/sangre , Enfermedad de Addison/diagnóstico , Síndrome de Cushing/sangre , Síndrome de Cushing/diagnóstico , Humanos , Control de Calidad , Radioinmunoensayo , Estándares de ReferenciaRESUMEN
OBJECTIVE: To determine the effects of muscle paralysis on aortic root blood flow in preterm infants with hyaline membrane disease. DESIGN: Each patient served as his/her own control in a prospectively controlled trial. SETTING: Neonatal ICU in a university hospital. PATIENTS: Ten ventilator-dependent preterm infants weighing 800 to 2820 g, 0 to 8 days of age, with hyaline membrane disease and seven control patients. INTERVENTIONS: Noninvasive measurement of aortic root blood flow by Doppler echocardiography 30 min before and 60 min after respiratory paralysis with 0.1 to 0.5 mg/kg of iv pancuronium, or following ventilator changes in control subjects. RESULTS: Mean aortic root blood flow increased significantly (p less than .001), from 212 to 276 mL/min.kg, accompanied by significant increases in stroke volume and heart rate. CONCLUSIONS: Pancuronium bromide may have a direct beneficial effect on the circulation of preterm infants with hyaline membrane disease.
Asunto(s)
Aorta/fisiopatología , Enfermedad de la Membrana Hialina/tratamiento farmacológico , Pancuronio/uso terapéutico , Velocidad del Flujo Sanguíneo/efectos de los fármacos , Presión Sanguínea/efectos de los fármacos , Dióxido de Carbono/sangre , Frecuencia Cardíaca/efectos de los fármacos , Humanos , Enfermedad de la Membrana Hialina/sangre , Enfermedad de la Membrana Hialina/fisiopatología , Recién Nacido , Oxígeno/sangre , Estudios Prospectivos , Resistencia Vascular/efectos de los fármacosRESUMEN
Bacteriophage lambda requires the lambda O and P proteins for its DNA replication. The rest of the replication proteins are provided by the Escherichia coli host. Some of these host proteins, such as DnaK, DnaJ, and GrpE, are heat shock proteins. Certain mutations in the dnaK, dnaJ, or grpE gene block lambda growth at all temperatures and E. coli growth above 43 degrees C. We have isolated bacterial mutants that were shown by Southern analysis to contain a defective, mini-Tn10 transposon inserted into either of two locations and in both orientations within the dnaJ gene. We have shown that these dnaJ-insertion mutants did not grow as well as the wild type at temperatures above 30 degrees C, although they blocked lambda DNA replication at all temperatures. The dnaJ-insertion mutants formed progressively smaller colonies at higher temperatures, up to 42 degrees C, and did not form colonies at 43 degrees C. The accumulation of frequent, uncharacterized suppressor mutations allowed these insertion mutants to grow better at all temperatures and to form colonies at 43 degrees C. None of these suppressor mutations restored the ability of the host to propagate phage lambda. Radioactive labeling of proteins synthesized in vivo followed by immunoprecipitation or immunoblotting with anti-DnaJ antibodies demonstrated that no DnaJ protein could be detected in these mutants. Labeling studies at different temperatures demonstrated that these dnaJ-insertion mutations resulted in altered kinetics of heat shock protein synthesis. An additional eight dnaJ mutant isolates, selected spontaneously on the basis of blocking phage lambda growth at 42 degrees C, were shown not to synthesize DnaJ protein as well. Three of these eight spontaneous mutants had gross DNA alterations in the dnaJ gene. Our data provide evidence that the DnaJ protein is not absolutely essential for E. coli growth at temperatures up to 42 degrees C under standard laboratory conditions but is essential for growth at 43 degrees C. However, the accumulation of extragenic suppressors is necessary for rapid bacterial growth at higher temperatures.
Asunto(s)
Proteínas Bacterianas/genética , Bacteriófago lambda/genética , Replicación del ADN , Escherichia coli/genética , Proteínas de Choque Térmico/genética , Mutación , Proteínas Bacterianas/aislamiento & purificación , ADN Bacteriano/genética , ADN Bacteriano/aislamiento & purificación , Electroforesis en Gel Bidimensional , Proteínas de Escherichia coli , Genotipo , Proteínas del Choque Térmico HSP40 , Proteínas de Choque Térmico/aislamiento & purificación , Fenotipo , Plásmidos , Mapeo Restrictivo , Transducción GenéticaRESUMEN
In the RA-1000, a random-access discrete analyzer, an inert fluorocarbon fluid is used to prevent interaction and carryover. Production-model instruments were evaluated in two laboratories with respect to determination of glucose, creatinine, total protein, inorganic phosphorus, cholesterol, alkaline phosphatase, lactate dehydrogenase, creatine kinase, gamma-glutamyltransferase, and aspartate and alanine aminotransferases. Within-run, among-run, and day-to-day (for 15 days) precision was assessed, and results were correlated with those obtained by the methods routinely in use in our departments. Precision was excellent, correlation acceptable.