RESUMEN
By their mechanism of action local anesthesia methods are divided into diffuse and vascular. Anesthesias are vascular at arteriolo-capillary-venous system level. Besides more than 2-fold efficacy increase, circulatory mechanism also contributes to enhancement of cardiovascular system reactions.
Asunto(s)
Anestesia Dental/métodos , Anestesia Local/métodos , Anestésicos Locales/administración & dosificación , Anestésicos Locales/farmacología , Sistema Cardiovascular/metabolismo , HumanosRESUMEN
By their mechanism of action local anaesthesia methods were divided into diffused and vascular. Intraosseous, intraseptal and intraligamental anesthaesias are vascular ones at capillary-venous system level. Circulatory mechanism besides effectiveness increased more than 2-fold and also promotes enhancement of cardiovascular system responses.
Asunto(s)
Anestesia Dental/métodos , Anestesia Local/métodos , Anestésicos Locales/administración & dosificación , Carticaína/administración & dosificación , Anestésicos Locales/farmacología , Sistema Cardiovascular/efectos de los fármacos , Carticaína/farmacología , Humanos , Monitoreo Fisiológico , Factores de TiempoRESUMEN
5 articaine preparations including besides 4% articaine with epinephrine 1:100 000 and 1:200 000, 4% articaine with epinephrine 1:400 000, 2% articaine 1:200 000 and articaine without a vasoconstrictor were investigated on 52 volunteers, dental students. It was established that new combinations of articaine with epinephrine had relatively high activity to anesthetize dental pulp which was comparable with classic agents but slightly inferior to them in all the criteria studied. Articaine without adrenaline had not practically induced pulpal analgesia.
Asunto(s)
Anestesia Dental/métodos , Anestésicos Locales/administración & dosificación , Carticaína/administración & dosificación , Epinefrina/administración & dosificación , Vasoconstrictores/administración & dosificación , Anestésicos Locales/farmacología , Carticaína/farmacología , Pulpa Dental/efectos de los fármacos , Epinefrina/farmacología , Humanos , Factores de Tiempo , Vasoconstrictores/farmacologíaRESUMEN
Microstructure of the surface and micro- and nanostructure of the internal surface of 3D porous carrier ElastoPHB-3D were studied by methods of electron microscopy and atomic force microscopy. Biological properties of ElastoPHB-3D samples were evaluated using culture of L929 mouse fibroblasts. High porosity and pore size of biodegradable matrixes ElastoPHB-3D and their good biofunctional properties as the substrate for cell culturing allow us to recommend ElastoPH-3D as a promising carrier for cell transplantation and creation of artificial organs.
Asunto(s)
Portadores de Fármacos/química , Nanoestructuras , Poliésteres/química , Microscopía de Fuerza Atómica , Microscopía Electrónica de Rastreo , Propiedades de SuperficieRESUMEN
The effects of autologous bone marrow stromal cells immobilized on ElastoPHB membranes on reparative processes were studied on a model of rat skeletal muscle injury. Bone marrow stromal cells inhibited substitute (sclerosing) regeneration and activated reparative (reconstructive) regeneration of tissues.
Asunto(s)
Células de la Médula Ósea/citología , Trasplante de Médula Ósea/métodos , Membranas Artificiales , Músculo Esquelético/fisiología , Regeneración/fisiología , Adenoviridae , Animales , Galactósidos , Vectores Genéticos/genética , Inmunohistoquímica , Indoles , Operón Lac/genética , Músculo Esquelético/lesiones , Ratas , Ratas Wistar , Células del Estroma/trasplante , beta-Galactosidasa/metabolismoRESUMEN
Transplantation of suspention of allogenic fibroblast-like mesenchymal stem cells (AFMSC) of the bone marrow and AFMSC immobilized on the biodegradable membrane (BM) stimulates healing of deep burn wounds compared to control (without cell transplantation) because these cells produce biologically active substances into the wound. AFMSC immobilized on BM activate repair processes in the wounds earlier. This may be due to their higher functional activity created in the monolayer by adequate intercellular interactions. To avoid complications caused by low diffuse properties of BM (protein precipitation), BM should be removed from the surface of the burn wound maximum 3 days after its application.
Asunto(s)
Materiales Biocompatibles/metabolismo , Quemaduras/terapia , Células Inmovilizadas , Células Madre Mesenquimatosas , Trasplante de Células Madre/métodos , Animales , Células de la Médula Ósea/citología , Quemaduras/patología , Técnicas de Cultivo de Célula/instrumentación , Técnicas de Cultivo de Célula/métodos , Fibroblastos/citología , Masculino , Células Madre Mesenquimatosas/citología , Ratas , Ratas WistarRESUMEN
The paper contains an analysis of thrombogeneticity of biomaterials in vitro with regard for the changing time of recalcification of the platelet-free and platelet-related human blood plasma after incubation with a studied sample. Two sizeless thrombogeneticity parameters are suggested for describing the thrombogeneticity of studied biomaterials irrespective of a incubation-medium composition. It was demonstrated by the example of glass granules, which were modified by various immobilized functional groups, that the measuring of re-calcification time performed after samples' incubation secures a more objective assessment of platelet-resistant properties of materials. The relative index of thrombogeneticity of biomaterials (with the above glass granules being the controls), unlike the standardized parameter, opens up the possibility to study comparatively the activating action exerted on the coagulating and anti-coagulating blood systems of the studied samples, which differ by the ratios of their surface squares to the incubation-medium volume.
Asunto(s)
Ensayo de Materiales/métodos , Agregación Plaquetaria , HumanosAsunto(s)
Procedimientos Quirúrgicos Cardíacos/métodos , Cardiopatías/diagnóstico por imagen , Procedimientos Quirúrgicos Cardíacos/historia , Cardiopatías/diagnóstico , Cardiopatías/historia , Historia del Siglo XX , Radiografía , Federación de Rusia , Stents , Terminología como Asunto , Estados UnidosRESUMEN
Activity of polyadenylate polymerase was studied in blood serum of patients with some forms of leukemia. In acute form of leukemia, the activity of the polymerase was increased 3-fold in blood serum as compared with normal state. Under conditions of chronic myeloleukemia the enzymatic activity was altered depending on the disease step with increase in leukemia development and reached the maximal values in blast crisis. The maximal enzymatic activity was found in myelodysplastic syndrome in patients with refractory anemia and excess of blasts.
Asunto(s)
Leucemia/enzimología , Polinucleotido Adenililtransferasa/sangre , Enfermedad Aguda , Anemia Refractaria con Exceso de Blastos/enzimología , Crisis Blástica , Humanos , Leucemia Mielógena Crónica BCR-ABL Positiva/enzimología , Síndromes Mielodisplásicos/enzimologíaRESUMEN
The distribution of alleles of DRB, DQB and DQA loci was investigated in 118 healthy donors from St. Petersburg. Said alleles were identified by the restriction fragment length polymorphism method using DRB, DQB and DQA probes after TaqI digestion and Southern blotting. The frequencies of said alleles were compared with those of a cohort of healthy donors living in Germany. A significantly higher frequency of DRB-17-2 was identified in the Russian donors, as compared with the German counterparts. Also, the Russian donors revealed a decrease in the frequency of DQB-1 (P = 0.01) and an increase in that of DQB-X allele (P = 0.05). An analysis of DRB, DQB and DQA locus a alleles adhesion in a sample of donors from St. Petersburg showed it to agree, in a large proportion of cases, with the literature data. A parallel study of immunological specificity of DR antigen was undertaken to compare the results of genetic and serologic typing; errors in DR specificity identification were found to have been made in 17.0%.
Asunto(s)
Alelos , Donantes de Sangre , Antígenos HLA-D/genética , Complejo Mayor de Histocompatibilidad/genética , Antígenos HLA-DQ/genética , Antígenos HLA-DR/genética , HumanosRESUMEN
Some differences were detected in the spectrum of DNA-binding proteins in blood serum of healthy volunteers and of patients with various forms of hemoblastoses. Two fractions of high molecular mass proteins were found to be main components of DNA-binding proteins in blood serum of both healthy persons and these patients. The DNA-binding proteins with a molecular mass less than 150 kDa were not detected under conditions of normal state, in myeloblast and lymphoblast forms of acute leukemia and in chronic phase of chronic myeloleukemia. However, additional fraction of DNA-binding proteins about 30-40 kDa was found in blast crisis of chronic myeloleukemia and in refractory anemia with increase of blast forms.
Asunto(s)
Anemia Refractaria con Exceso de Blastos/sangre , Proteínas Sanguíneas/metabolismo , Proteínas de Unión al ADN/metabolismo , Leucemia Mielógena Crónica BCR-ABL Positiva/sangre , Leucemia/sangre , Enfermedad Aguda , HumanosRESUMEN
An automated data bank of individual dosimetric control using Electronika MS 0507 microcomputer has been developed and is being tested now. The bank provides for data acquisition and processing, registration of the professional status of personnel, calculations, automated archives, and printing of documents. Radiation control data, accumulated at the data bank, were used to determine approaches to control a radiation situation by correcting parameters of a technological process.
Asunto(s)
Bases de Datos Factuales , Dosis de Radiación , Protección Radiológica , Humanos , MicrocomputadoresRESUMEN
Adenyl cyclase activity was estimated in blood platelets of healthy persons and of patients with myeloleukosis, osteomyelofibrosis and with Glanzmann's thrombasthenia. Six-fold decrease in the adenyl cyclase activity from the platelets was observed in chronic myeloleukosis. The enzymatic activity in platelets was similar to normal level in osteomyelofibrosis and Glanzmann's thrombasthenia.
Asunto(s)
Adenilil Ciclasas/metabolismo , Plaquetas/enzimología , Leucemia Mieloide/sangre , Mielofibrosis Primaria/sangre , Púrpura Trombocitopénica/genética , Humanos , Técnicas In Vitro , Púrpura Trombocitopénica/sangre , SíndromeRESUMEN
The presence of a fraction of oligonucleotides rich in adenin, and a high metabolic activity of this fraction was demonstrated. Data are presented on the participation of adenin-rich oligonucleotides of human platelets in the blood coagulation system reactions, namely in the release reaction induced by thrombin.