RESUMEN
BACKGROUND: Our survey has found eleven plants that have being consumed for traditional treatment of diabetes mellitus, particularly in Saudi Arabia and generally in many countries across the world. The literature reported about trace elements such as Cr and Mn positively affecting diabetes mellitus. The aim of this work is to determine the total, accessible element species, and protein-fraction contents of Cr and Mn in the edible parts of those plants. METHODS: The total contents of Mn were determined by inductively coupled plasma-optical emission spectrometry (ICP-OES), while the total contents of Cr was determined by ICP-sector field (sf)-mass spectrometry (MS) due to lower concentration. The protein-fraction contents were determined in accessible element species by size-exclusion chromatography (SEC)-ICP-MS. RESULTS AND DISCUSSION: The separation was successfully carried out to quantify Cr and Mn bound up to 11 protein fractions. The examined plants recorded wide ranges of total contents (Cr 44.7-1880.0⯵g/kg and Mn 3.7-59.0â¯mg/kg) and accessible element species contents (Cr 0.93-29.40⯵g/kg and Mn 0.82-35.85â¯mg/kg). Also wide ranges of percentages of accessible element species contents to total contents of Cr (0.65-4.21%) and Mn (5.43-68.42%) were obtained. The hazardous indices of both trace elements Cr and Mn for all examined plants consumed by both children and adults were all < 1, indicating no probability of health risk to occur. Moreover, Cr as carcinogen element reported no probability of cancer risk to occur from the consumption of all examined plants. Irrespective of plant species, Cr was quantified in all SEC fractions (mainly protein fractions), with the exception of 1.9-3.7â¯kDa, while Mn was quantified in all SEC fractions with the exception of 100-120 and 1.3-3.7â¯kDa. Nevertheless, the majority of accessible Cr species contents bound to the 10-14 and 0.05-0.40â¯kDa fractions, while that of Mn bound to 0.05-0.40â¯kDa fraction. To gather, the benefits of specific plant species in terms of accessible Cr and Mn species contents, in addition to accessible Zn species contents reported in our previous study, Haloxylon Salicornicum, Olea Europaea Momordica and Charantia are recommended to be consumed for traditionally controlling T2DM.
Asunto(s)
Plantas Medicinales/química , Oligoelementos/análisis , Cromatografía en Gel , Cromo/análisis , Manganeso/análisis , Espectrometría de Masas , Proteínas de Plantas/química , Arabia SauditaRESUMEN
This study aims at the assessment of total zinc contents, water zinc extract contents and zinc-protein profile in medicinal plants traditionally used for diabetes treatment. While zinc-protein profile was screened in plant samples using the online coupling of size exclusion chromatography-inductively coupled plasma-mass spectrometry (SEC-ICP-MS), total zinc contents and zinc water extract contents were determined using inductively coupled plasma-optical emission spectrometry (ICP-OES). The analysis of two certified reference materials with similar matrix for total zinc content revealed recovery values of 97.1% and 100.5% while the average of recovery of the summed Zn concentrations from protein fractions compared to total Zn was 103.0 ± 4.8%. Based on the FAO/WHO classification, Sesamum indicum, Nigella sativa, Trigonella Foenum-graecum and Pennisetum glaucum are classified as highest Zn-content. For protein profile, zinc was quantified in 330-430 and 50-60 kDa fractions of all examined plants while no contents were quantified in the inorganic fraction 0.05-0.4 kDa of all plant species. Also, only three plant species recorded Zn contents in the phytate fraction (0.9-1.5 kDa fraction). The fruits of the Momordica Charantia and the Citrullus colocynthis were with the highest extractable zinc concentration; 13.55 ± 0.45 and 10.08 ± 0.63 mg/kg, respectively. The highest Zinc capturing capacity was reported for the 50-60 and 70-87 KDa protein fractions.
Asunto(s)
Diabetes Mellitus/tratamiento farmacológico , Proteínas de Plantas/química , Plantas Medicinales/química , Zinc/análisis , Humanos , Análisis EspectralRESUMEN
This study was performed to investigate the influence of cancer on selected trace elements among Sudanese patients with confirmed breast cancer. Instrumental neutron activation analysis was used to estimate contents of Se, Zn, Fe, Cr, Rb, Cs, Co and Sc in 40 subjects. Wilcoxon signed ranks test was used to examine if there was any difference in the concentrations of elements from normal and malignant tissues. It was found that Se, Zn and Cr elements from the malignant tissues are significantly elevated (P < 0.05) compared to the normal tissue. The results obtained have shown consistency with results obtained by some previous studies.