Asunto(s)
Factores de Crecimiento Nervioso/sangre , Enfermedades del Sistema Nervioso/sangre , Adulto , Anciano , Anticuerpos Monoclonales , Trasplante de Tejido Encefálico , Trasplante de Tejido Fetal , Humanos , Técnicas para Inmunoenzimas , Persona de Mediana Edad , Degeneración Nerviosa/fisiología , Enfermedades del Sistema Nervioso/cirugía , Enfermedad de Parkinson/sangre , Enfermedad de Parkinson/cirugíaRESUMEN
The two-site enzyme immunoassasy (EIA) using the monoclonal antibody (MAb) 27/21 is a valuable method capable of detecting mouse and human NGF quantitatively (Soderstrom et al., 1990). The presence of NGF in serum has been controversial, since t6he previous assay methods failed to detect circulating NGF. Recently, we described the immunological detection of low levels of NGF in human serum samples and introduced a blocking test validating the specificity of the immunoreactivity for NGF in human serum (Lorigados et al., 1982). In the present work, we applied this two-site EIA using monoclonal NGF antibody 27/21 in the study of NGF serum levels from diverse neurodegenerative disorders. We also studied evolutive samples of Parkinson's patients that received neural transplant (AU)
Asunto(s)
Humanos , Factores de Crecimiento Nervioso , Enfermedad de Alzheimer/inmunología , Enfermedad de Parkinson/inmunología , Esclerosis Amiotrófica Lateral/inmunología , Esclerosis Múltiple/inmunología , Enfermedad de Huntington/inmunologíaRESUMEN
The two-site enzyme immunoassasy (EIA) using the monoclonal antibody (MAb) 27/21 is a valuable method capable of detecting mouse and human NGF quantitatively (Soderstrom et al., 1990). The presence of NGF in serum has been controversial, since t6he previous assay methods failed to detect circulating NGF. Recently, we described the immunological detection of low levels of NGF in human serum samples and introduced a blocking test validating the specificity of the immunoreactivity for NGF in human serum (Lorigados et al., 1982). In the present work, we applied this two-site EIA using monoclonal NGF antibody 27/21 in the study of NGF serum levels from diverse neurodegenerative disorders. We also studied evolutive samples of Parkinson's patients that received neural transplant
Asunto(s)
Humanos , Enfermedad de Alzheimer/inmunología , Esclerosis Amiotrófica Lateral/inmunología , Esclerosis Múltiple/inmunología , Enfermedad de Huntington/inmunología , Factores de Crecimiento Nervioso , Enfermedad de Parkinson/inmunologíaRESUMEN
Previous studies have shown that extracts from the target optic tectum stimulate neurite outgrowth from retinal explants. The present study indicates that the choroid coat is an even richer source of retinotrophic activity. We thus studied the effects of recombinant rat ciliary neurotrophic factor (CNTF) on primary cultures of dissociated chick ciliary ganglion neurons and retinal explants for a comparison with choroid coat extract from the E18 chick. For our assays, E9 ciliary neurons were incubated in collagen gels and retinal explants were cultured on collagen gels with the addition of the trophic factors and maintained for two or four days. Survival of ciliary neurons per area as well as maximal neurite length in retinal cultures were determined. Growth responses occurred in a dose-dependent manner both to CNTF and choroid extract. Immunofluorescence examination of cells and developing processes showed 200 kdal neurofilament positivity demonstrating that the cells studied were neurons with neurites. It is concluded that a trophic activity of the choroid as well as the recombinant CNTF stimulate retinal neuron survival and neurite extension. The results suggest that CNTF may have developmental functions in the establishment of the visual pathways.