RESUMEN
Kae1 is a subunit of the highly evolutionarily conserved KEOPS/EKC complex, which is involved in universal (t6A37) tRNA modification. Several reports have discussed the participation of this complex in transcription regulation in yeast and human cells, including our previous observations of KaeA, an Aspergillus nidulans homologue of Kae1p. The aim of this project was to confirm the role of KaeA in transcription, employing high-throughput transcriptomic (RNA-Seq and ChIP-Seq) and proteomic (LC-MS) analysis. We confirmed that KaeA is a subunit of the KEOPS complex in A. nidulans. An analysis of kaeA19 and kaeA25 mutants showed that, although the (t6A37) tRNA modification is unaffected in both mutants, they reveal significantly altered transcriptomes compared to the wild type. The finding that KaeA is localized in chromatin and identifying its protein partners allows us to postulate an additional nuclear function for the protein. Our data shed light on the universal bi-functional role of this factor and proves that the activity of this protein is not limited to tRNA modification in cytoplasm, but also affects the transcriptional activity of a number of nuclear genes. Data are available via the NCBI's GEO database under identifiers GSE206830 (RNA-Seq) and GSE206874 (ChIP-Seq), and via ProteomeXchange with identifier PXD034554 (proteomic).
Asunto(s)
Aspergillus nidulans , Aspergillus nidulans/genética , Aspergillus nidulans/metabolismo , Cromatina/metabolismo , Humanos , Proteómica , ARN de Transferencia/genética , ARN de Transferencia/metabolismo , Saccharomyces cerevisiae/metabolismoRESUMEN
In Aspergillus nidulans, nitrogen and carbon metabolism are under the control of wide-domain regulatory systems, including nitrogen metabolite repression, carbon catabolite repression and the nutrient starvation response. Transcriptomic analysis of the wild type strain grown under different combinations of carbon and nitrogen regimes was performed, to identify differentially regulated genes. Carbon metabolism predominates as the most important regulatory signal but for many genes, both carbon and nitrogen metabolisms coordinate regulation. To identify mechanisms coordinating nitrogen and carbon metabolism, we tested the role of AreB, previously identified as a regulator of genes involved in nitrogen metabolism. Deletion of areB has significant phenotypic effects on the utilization of specific carbon sources, confirming its role in the regulation of carbon metabolism. AreB was shown to regulate the expression of areA, tamA, creA, xprG and cpcA regulatory genes suggesting areB has a range of indirect, regulatory effects. Different isoforms of AreB are produced as a result of differential splicing and use of two promoters which are differentially regulated by carbon and nitrogen conditions. These isoforms are likely to be functionally distinct and thus contributing to the modulation of AreB activity.
Asunto(s)
Aspergillus nidulans/metabolismo , Carbono/metabolismo , Proteínas Fúngicas/metabolismo , Factores de Transcripción GATA/metabolismo , Regulación Fúngica de la Expresión Génica , Nitrógeno/metabolismo , Aspergillus nidulans/genética , Proteínas Fúngicas/genética , Factores de Transcripción GATA/genética , Regiones Promotoras GenéticasRESUMEN
The kaeA(KAE1) (suDpro) gene, which was identified in Aspergillus nidulans as a suppressor of proline auxotrophic mutations, encodes the orthologue of Saccharomyces cerevisiae Kae1p, a member of the evolutionarily conserved KEOPS/EKC (Kinase, Endopeptidase and Other Proteins of Small size/Endopeptidase-like and Kinase associated to transcribed Chromatin) complex. In yeast, this complex has been shown to be involved in tRNA modification, transcription, and genome maintenance. In A. nidulans, mutations in kaeA result in several phenotypic effects, the derepression of arginine catabolism genes, and changes in the expression levels of several others, including genes involved in amino acid and siderophore metabolism, sulfate transport, carbon/energy metabolism, translation, and transcription regulation, such as rcoA(TUP1), which encodes the global transcriptional corepressor.
Asunto(s)
Arginina/metabolismo , Aspergillus nidulans/genética , Proteínas Fúngicas/fisiología , Secuencia de Aminoácidos , Aspergillus nidulans/metabolismo , Secuencia de Bases , Regulación Fúngica de la Expresión Génica , Genes Fúngicos , Pleiotropía Genética , Redes y Vías Metabólicas , Datos de Secuencia Molecular , Complejos Multiproteicos/fisiología , MutaciónRESUMEN
Differential regulation of transcript stability is an effective means by which an organism can modulate gene expression. A well-characterized example is glutamine signalled degradation of specific transcripts in Aspergillus nidulans. In the case of areA, which encodes a wide-domain transcription factor mediating nitrogen metabolite repression, the signal is mediated through a highly conserved region of the 3' UTR. Utilizing this RNA sequence we isolated RrmA, an RNA recognition motif protein. Disruption of the respective gene led to loss of both glutamine signalled transcript degradation as well as nitrate signalled stabilization of niaD mRNA. However, nitrogen starvation was shown to act independently of RrmA in stabilizing certain transcripts. RrmA was also implicated in the regulation of arginine catabolism gene expression and the oxidative stress responses at the level of mRNA stability. ΔrrmA mutants are hypersensitive to oxidative stress. This phenotype correlates with destabilization of eifE and dhsA mRNA. eifE encodes eIF5A, a translation factor within which a conserved lysine is post-translationally modified to hypusine, a process requiring DhsA. Intriguingly, for specific transcripts RrmA mediates both stabilization and destabilization and the specificity of the signals transduced is transcript dependent, suggesting it acts in consort with other factors which differ between transcripts.
Asunto(s)
Aspergillus nidulans/genética , Regulación de la Expresión Génica , Nitrógeno/metabolismo , Estrés Oxidativo , Estabilidad del ARN , Proteínas de Unión al ARN/metabolismo , Arginina/metabolismo , Eliminación de Gen , Glutamina/metabolismo , Proteínas de Unión al ARN/genéticaRESUMEN
The filamentous fungus Aspergillus nidulans can utilize arginine both as a nitrogen and carbon source. Analysis of areA and areB single and double mutants has shown that the two GATA transcription factors AREA and AREB negatively regulate the expression of arginine catabolism genes agaA and otaA under nitrogen repressing conditions. AREA is necessary for the ammonium repression of agaA and otaA under carbon repressing conditions, while AREB is involved under carbon-limiting conditions. The ability of both AREA and AREB to sense the status of carbon metabolism is most probably dependent on NMRA, and not on the transcription factor CREA, which mediates general carbon catabolite repression in A. nidulans. NMRA is a co-repressor which has previously been shown to bind the C-terminus of AREA and inhibits its activity under conditions of nitrogen sufficiency, in response to high intracellular glutamine levels. We therefore propose a novel function for NMRA, the modulation of AREA and AREB activity in response to the carbon status of the cell.
Asunto(s)
Arginina/metabolismo , Aspergillus nidulans/genética , Aspergillus nidulans/metabolismo , Carbono/metabolismo , Regulación hacia Abajo , Proteínas Fúngicas/metabolismo , Factores de Transcripción GATA/metabolismo , Nitrógeno/metabolismo , Proteínas Represoras/metabolismo , Factores de Transcripción/metabolismo , Proteínas Fúngicas/genética , Factores de Transcripción GATA/genética , Regulación Fúngica de la Expresión Génica , Proteínas Represoras/genética , Factores de Transcripción/genéticaRESUMEN
Structural and functional diversity of cellular types is mainly the result of combinatorial regulation of gene expression in which transcriptional factors interact in different ways and different combinations in response to signaling pathways. MADS-box transcription factors are found in all eukaryotic kingdoms and they are of key importance in signal transduction to the genome. MADS-box factors interact with other regulatory proteins in complexes which can activate or repress transcription. In many cases these additional proteins influence the affinity and specificity of the complex for the target sequence. They can change the chromatin structure and decide which group of genes is regulated in a specific way. The MADS domain is a distinctive feature of the MADS-box transcription factors. It is a highly conserved domain of approximately 80 amino acids, responsible for DNA binding and bending, dimerization and interactions with other proteins. In animal cells MADS-box transcription factors participate in regulation of cell growth and differentiation, embryogenesis and morphogenesis. In yeast they are involved in the control of cell cycle progression, regulation of mating type specific genes, control of arginine metabolism, cell wall biosynthesis and osmotic stress response. Plant MADS-box proteins provide different homeotic functions determining floral organ development. This review is aimed at characterizing how MADS-box transcription factors interact with other regulatory proteins and how they are activated by different signal transduction pathways. We also summarize recent theories concerning the evolution of this family of transcriptional regulators.
Asunto(s)
Hongos/genética , Proteínas de Dominio MADS/metabolismo , Plantas/genética , Activación Transcripcional/genética , Animales , Ciclo Celular/genética , Transducción de Señal/genéticaRESUMEN
Expression of Aspergillus nidulans arginine catabolism genes, agaA and otaA, is regulated at the level of transcription by a specific induction and two global carbon and nitrogen repression systems. Post-transcriptional and/or post-translational mechanisms have also been proposed to operate additionally. Gene tagging with transposon impala allowed us to select the rrmA gene. RRMA protein contains three conserved RRM domains, typical for RNA-binding proteins. The gene has a complex structure with several potential transcription start sites, an exceptionally long intron in 5'UTR and few uORFs in the intron. RRMA is highly conserved among fungi. Its homologues, Csx1p of Schizosaccharomyces pombe and Ngr1p of Saccharomyces cerevisiae, participate in the post-transcriptional regulation of specific genes by modifying transcript stability. Levels of otaA and agaA transcripts in the rrmA::impala loss of function mutant grown under inducing conditions are significantly higher than in the wild type strain. We propose that RRMA participates in a mechanism promoting agaA and otaA mRNA degradation. The rrmA::impala mutation has pleiotropic character and results in a slow growth phenotype indicating that rrmA functions are not limited to the regulation of arginine catabolism.
Asunto(s)
Arginina/metabolismo , Aspergillus nidulans/genética , Proteínas Fúngicas/genética , Proteínas de Unión al ARN/genética , Secuencia de Aminoácidos , Arginasa/genética , Arginasa/metabolismo , Aspergillus nidulans/metabolismo , Secuencia de Bases , Northern Blotting , Proteínas Fúngicas/metabolismo , Regulación Fúngica de la Expresión Génica , Modelos Biológicos , Datos de Secuencia Molecular , Mutación , Ornitina/metabolismo , Reacción en Cadena de la Polimerasa , Regiones Promotoras Genéticas , Proteínas de Unión al ARN/metabolismo , Homología de Secuencia de Aminoácido , Transcripción GenéticaRESUMEN
The arginine catabolism gene otaA encoding ornithine transaminase (OTAse) is specifically induced by arginine and is under the control of the broad-domain carbon and nitrogen repression systems. Arginine induction is mediated by a product of arcA gene coding for Zn(2)C(6) activator. We have identified a region responsible for arginine induction in the otaA promoter (AnUAS(arg)). Deletions within this region result in non-inducibility of OTAse by arginine, whether in an arcA(+) strain or in the presence of the arcA(d)47 gain of function allele. AnUAS(arg) is very similar to the Saccharomyces cerevisiae UAS(arg), a sequence bound by the Zn(2)C(6) activator (ArgRIIp), acting in a complex with two MADS-box proteins (McmIp and ArgRIp). We demonstrate here that two CREA in vitro binding sites in the otaA promoter are functional in vivo. CREA is directly involved in carbon repression of the otaA gene and it also reduces its basal level of expression. Although AREA binds to the otaA promoter in vitro, it probably does not participate in nitrogen metabolite repression of the gene in vivo. We show here that another putative negatively acting GATA factor AREB participates directly or indirectly in otaA nitrogen repression. We also demonstrate that the high levels of OTAse activity are an important factor in the suppression of proline auxotrophic mutations. This suppression can be achieved neither by growing of the proline auxotroph under carbon/nitrogen derepressing conditions nor by introducing of a creA(d) mutation.