RESUMEN

Search vectors composed of Gly, Ala, Arg, and Pro (GARP) residues retrieve 98% of each of the ribosomal proteins in prokaryotic species with no false hits. Different combinations of G, A, R and P and insertions differentiate each ribosomal protein from all others. Amino acids in two sequence positions separate Gram+ from Gram- bacteria. Specific residues separate proteins of cyanobacteria and chloroplasts from all other species. Structural information played an essential role in developing a GARP based technique to achieve perfect sequence alignment. It is possible to understand why GARP residues are 100% conserved in specific positions in families of proteins present in all species.

Asunto(s)

Proteínas Bacterianas/genética , Evolución Molecular , Proteínas Ribosómicas/genética , Secuencia de Aminoácidos , Aminoácidos/genética , Cloroplastos/metabolismo , Secuencia Conservada , Cianobacterias/genética , Cianobacterias/metabolismo , Datos de Secuencia Molecular , Alineación de Secuencia