RESUMEN

The effect of aluminum on dimorphic fungi Yarrowia lipolytica was investigated. High aluminum (0.5-1.0 mM AlK(SO(4))(2)) inhibits yeast-hypha transition. Both vanadate-sensitive H(+) transport and ATPase activities were increased in total membranes isolated from aluminum-treated cells, indicating that a plasma membrane H(+) pump was stimulated by aluminum. Furthermore, Al-treated cells showed a stronger H(+) efflux in solid medium. The present results suggest that alterations in the plasma membrane H(+) transport might underline a pH signaling required for yeast/hyphal development. The data point to the cell surface pH as a determinant of morphogenesis of Y. lipolytica and the plasma membrane H(+)-ATPase as a key factor of this process.

Asunto(s)

Aluminio/farmacología , Transporte Iónico/efectos de los fármacos , Morfogénesis/efectos de los fármacos , ATPasas de Translocación de Protón/fisiología , Yarrowia/efectos de los fármacos , Membrana Celular/química , Membrana Celular/efectos de los fármacos , Concentración de Iones de Hidrógeno , Hifa/efectos de los fármacos , Hifa/crecimiento & desarrollo , Hifa/metabolismo , Transducción de Señal , Yarrowia/química , Yarrowia/crecimiento & desarrollo