RESUMEN
The purpose of the present report was to estimate the population parameters of cortisol concentrations in urine, an endogenous hormone used as a 'doping' agent and for which an international threshold (1.0 micrograms/ml) has been proposed. Two data bases (French and UK) corresponding to 112 and 142 samples, respectively were considered. Urine was collected under specific post competition conditions. Cortisol concentrations were obtained by validated methods (HPLC for the French samples, and GC-MS for UK samples). No difference was observed between the 2 data sets and statistical analyses were carried out on the two merged files. The overall geometric mean cortisol concentration was 48 ng/ml. Distribution was not Gaussian. A log-normal distribution was not rejected (for P > 0.05). Using the log-normal distribution, it was calculated that the probability of exceeding a cortisol concentration in urine of 1.0 micrograms/ml was 1.1 x 10(-4). It was concluded that the actual international threshold is specific i.e. robust with regard to the risk of erroneously declaring an unmedicated horse as positive.
Asunto(s)
Doping en los Deportes , Caballos/orina , Hidrocortisona/orina , Animales , Cromatografía Líquida de Alta Presión , Francia , Cromatografía de Gases y Espectrometría de Masas , Reino UnidoAsunto(s)
Enzimas , Medicina Legal , Preparaciones Farmacéuticas/análisis , Suelo/análisis , Adulto , Huesos/análisis , Femenino , HumanosRESUMEN
The device described replaces the sampling probe of any automatic diluter. It enables septum-sealed blood vials to be sampled without exposing the blood to the atmosphere, and dispenses the blood into sealed reaction vials. The advantages are that loss of volatiles in the sample and exposure of operators to open blood containers are eliminated.