RESUMEN

The effects of a parasitic infection with the nematode Nippostrongylus brasiliensis on the degradation rates of cytoplasmic tRNA, rRNA and mRNA in rats have been investigated by measuring the renal excretion rates of the modified RNA catabolites N6-threoninocarbonyladenosine, pseudouridine and 7-methylguanine. Between days 9 and 13 post-infection when the expulsion of N. brasiliensis is usually the most pronounced, the degradation rates of the different RNA classes were significantly higher than in the control rats (P < 0.05) by, on average, +24% (tRNA), +34% (rRNA) and +26% (mRNA). We suspect that the elevated degradation rates of RNA are related to an increased production of reactive oxygen species by the host during the expulsion of N. brasiliensis.

Asunto(s)

Nippostrongylus/fisiología , ARN Mensajero/metabolismo , ARN Ribosómico/metabolismo , ARN de Transferencia/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Infecciones por Strongylida/metabolismo , Animales , Cromatografía Líquida de Alta Presión , Interacciones Huésped-Parásitos , Masculino , ARN Mensajero/orina , ARN Ribosómico/orina , ARN de Transferencia/orina , Ratas , Ratas Wistar , Estadísticas no Paramétricas , Infecciones por Strongylida/genética , Infecciones por Strongylida/parasitología , Infecciones por Strongylida/orina