RESUMEN
Background: Recent studies have put forward the viewpoint of "bone immunology", which holds that the immune system and immune factors play an important regulatory role in the occurrence and development of osteoporosis. This study was intended to identify genetic characteristics of differentially expressed immune-related mRNA and lncRNA in patients combined with osteoporosis and vertebral fracture. Methods: The peripheral blood samples were obtained from 3 groups of subjects: healthy control (HC), osteoporosis patients without vertebral fracture (OWF), and osteoporosis patients combined with vertebral fracture (OVF). The data were integrated to obtain differentially expressed mRNAs (DEmRNAs) and differentially expressed lncRNAs (DElncRNAs). Subsequently, the protein-protein interaction (PPI) networks were constructed. Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) enrichment analyses were performed. Cytoscape-cytoHubba plug-in was used to identify key DEmRNAs. Furthermore, lncRNA-miRNA-mRNA, mRNA-lncRNA co-expression and transcription factors (TFs) networks were constructed. In addition, real-time PCR verification was performed. Results: Totally of 3378 lncRNA-mRNA pairs were obtained, and the lncRNA co-expressed mRNA was mainly enriched in immune-related pathways, especially in GO-biological process (GO-BP) analysis. A total of 8 hub immune-related DEmRNAs were obtained, including IL18R1, IL18RAP, SLC11A1, CSF2RA, CCR3, IL1R2, PGLYRP1, and IL1R1. The TFs network showed that 8 hub immune-related DEmRNAs had interacting TFs. The co-expression network showed that 7 hub immune-related DEmRNAs (IL18R1, IL18RAP, SLC11A1, CSF2RA, IL-1R2, PGLYRP1, and IL1R1) had lncRNA-mRNA co-expression relationship. In addition, the lncRNA-miRNA-mRNA network includes 32 miRNAs, 7 hub immune-related mRNAs (IL18R1, IL18RAP, CSF2RA, CCR3, IL1R2, PGLYRP1, and IL1R1), and 11 lncRNAs. Conclusion: Our study provides a novel and in-depth identification of co-expressed mRNAs and lncRNAs in patients combined with osteoporosis and vertebral fracture at a molecular level. This may provide new candidate biomarkers for the diagnosis of patients with high-risk fractures in the future.
Asunto(s)
MicroARNs , Osteoporosis , ARN Largo no Codificante , Fracturas de la Columna Vertebral , Humanos , ARN Largo no Codificante/genética , ARN Largo no Codificante/metabolismo , Transcriptoma , ARN Mensajero/genética , ARN Mensajero/metabolismo , Redes Reguladoras de Genes , MicroARNs/genética , MicroARNs/metabolismo , Osteoporosis/genéticaRESUMEN
BACKGROUND: High tibial osteotomy (HTO) is used to treat medial degeneration of the osteoarthritis (OA) knee. However, shortcomings still exist in the current procedure, like unprecise creation, inability to correct knee rotation, and internal fixed failure. Here, we reported a novel procedure: patient-specific 3D-printed plates for opening wedge high tibial osteotomy (OWHTO) combined with Taylor spatial frame (TSF). The detailed technique was described, and the clinical outcomes were evaluated. METHODS: We prospectively evaluate outcomes of patient-specific 3D-printed plates for OWHTO with use of TSF in 25 patients with knee OA and varus alignment. Postoperative efficacy was evaluated using the HSS knee score, pain visual simulation score (VAS), and knee joint motion (ROM), and lower limb alignment was evaluated by measuring femorotibial angle (FTA) and hip-knee-ankle (HKA). Results and Conclusion. All patients did not experience complications such as wound infection, nerve damage, or bone amputation. 25 patients were followed up for 6-18 months. The bony union at bone amputation was achieved in 3 months after surgery, and the pain symptoms were significantly alleviated or disappeared. The VAS score was significantly reduced in 6 months after surgery compared with preoperative; the HSSS score was significantly added in 6 months after surgery compared with preoperative. The ROM of knee joint increased significantly 6 months after operation compared with that before operation, and the difference was statically significant (P < 0.05). The FTA and HKA after operation were significantly superior to that before operation, and the difference was statically significant (P < 0.01). CONCLUSIONS: Our study showed that patient-specific 3D-printed plates for HTO with the use of TSF have the advantages of small trauma, few complications, simple operation, and fast recovery in treating knee OA and varus alignment.
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Osteoartritis de la Rodilla , Humanos , Articulación de la Rodilla , Osteoartritis de la Rodilla/complicaciones , Osteoartritis de la Rodilla/cirugía , Osteotomía , Impresión Tridimensional , Estudios Retrospectivos , Tibia/cirugíaRESUMEN
Long noncoding RNAs (lncRNAs) contribute to the tumorigeneses of numerous types of cancer, including glioma. The present study was designed to unveil a novel lncRNA functioning in glioma and explore the underlying mechanisms. lncRNA titinantisense RNA1 (TTNAS1), miR27b3p and Runtrelated transcription factor 1 (RUNX1) expression in glioma tissues and cell lines was estimated by RTqPCR. SiTTNAS1 was transfected into glioma cell lines (U251 and LN229), and CCK8 assay, flow cytometry, wound healing and Transwell assays were applied to estimate the function of TTNAS1 in glioma cells. miR27b3p inhibitor was used to explore the mechanisms. The results revealed that TTNAS1 was highly expressed in glioma specimens and cell lines. Downregulation of TTNAS1 inhibited the proliferation, migration and invasion of the glioma cells, as well as increased the rate of apoptosis. In vivo, the tumor growth was also inhibited by TTNAS1 depletion in nude mice. Furthermore, we revealed that TTNAS1 exerted oncogenic effects via sponging miR27b3p and thereby positively regulating RUNX1 expression. In conclusion, the present study supported that TTNAS1 acts as an oncogene in glioma by targeting miR27b3p to release RUNX1. This finding may contribute to gene therapy of glioma.
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Neoplasias Encefálicas/genética , Subunidad alfa 2 del Factor de Unión al Sitio Principal/genética , Glioma/genética , MicroARNs/metabolismo , ARN Largo no Codificante/metabolismo , Animales , Apoptosis/genética , Encéfalo/patología , Encéfalo/cirugía , Neoplasias Encefálicas/patología , Neoplasias Encefálicas/cirugía , Línea Celular Tumoral , Movimiento Celular/genética , Proliferación Celular/genética , Progresión de la Enfermedad , Femenino , Regulación Neoplásica de la Expresión Génica , Glioma/patología , Glioma/cirugía , Humanos , Ratones , Oncogenes , Ensayos Antitumor por Modelo de XenoinjertoRESUMEN
OBJECTIVE: To evaluate the effects of preoperative simulation based on patient-specific vertebral models, and computer-assisted designed drill navigation templates in expansive open-door laminoplasty (EOLP). METHODS: We retrospectively analyzed 18 consecutive patients who underwent EOLP, 11 patients (45 vertebrae) were treated with traditional EOLP (traditional group) and 7 patients (28 vertebrae) were treated with EOLP aided by the drill navigation template and preoperative simulation (template group). We assessed the operation time, intraoperative blood loss, hospitalization expenses, visual analog scale (VAS), Japanese orthopedic association (JOA), neck disability index (NDI), Pavlov's ratio, laminoplasty opening size (LOS), hinge fracture rate and trough positions. RESULTS: The operation time of template group (98.6 ± 13.0 minutes) was significantly shorter (P < 0.05) than that of traditional group (125.4 ± 13.2 minutes). Both groups achieved similar hospitalization expenses and significant improvement in VAS, JOA score, NDI, and Pavlov ratio. The postoperative hinge fracture rate of template group (0%; 0/28) was significantly lower (P < 0.05) than that of traditional group (11.1%, 5/45). In template group, all the troughs (56/56 [100%]; 28/28 on the hinge side and 28/28 on the open side), while in traditional group, only 72.2% (65/90; 31/45 on the hinge side and 34/45 on the hinge side) of the troughs were at the proper hinge position. CONCLUSIONS: Drill navigation template and preoperative simulations in EOLP are technically feasible. With the help of templates and preoperative simulation, shorten operation time, accurate trough positioning, decrease incidence of hinge fracture and could be acquired. This technique should be further studied and affirmed in clinical applications.
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Vértebras Cervicales/cirugía , Laminectomía , Laminoplastia , Resultado del Tratamiento , Adulto , Anciano , Femenino , Humanos , Laminectomía/métodos , Laminoplastia/métodos , Masculino , Persona de Mediana Edad , Tempo Operativo , Periodo Posoperatorio , Estudios Retrospectivos , Factores de TiempoRESUMEN
Chondrosarcoma is a common malignant bone tumor, which accounts for 20% of all malignant bone tumors. It often occurs in the long bones, but the incidence of scapular chondrosarcoma is rare. Here, we describe a case of a large chondrosarcoma occurring in the scapula which was treated with Malawer limb salvage surgery. The patient retained considerable limb function after complete removal of the tumor tissue as assessed at the follow-up visit two years and ten months following surgery.
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Neoplasias Óseas/cirugía , Condrosarcoma/cirugía , Recuperación del Miembro , Terapia Recuperativa , Escápula/cirugía , Neoplasias Óseas/patología , Condrosarcoma/patología , Humanos , Masculino , Persona de Mediana Edad , Escápula/patología , Resultado del TratamientoRESUMEN
OBJECTIVE: Posterolateral intertransverse process fusion was performed in aged and young adult female rabbits lumbar spine using recombinant human bone morphogenetic protein-2 (rhBMP-2) and autograft to reveal the function of rhBMP-2 on spinal fusion on aged animals. METHODS: A total of 24 female New Zealand white rabbits included 12 young adult of 6 months and 12 aged of 2-year-old, was divided into 4 groups: (1) young adult autologous iliac crest bone group [ICBG(Y), n=6); (2) young adult rhBMP-2/absorbable collagen sponge (ACS) group [BMP-2(Y), n=6]; (3) aged autologous iliac crest bone group [ICBG(O), n=6]; aged rhBMP-2/ACS group [BMP-2(O), n=6]. All were underwent posterolateral fusion in same day. rhBMP-2 and autologous iliac crest bone was implant bilateral LS-L6 intertransverse processes, respectively. Half of the rabbits were sacrificed at 3.6 weeks following surgery, respectively. The results were assessed by manual palpation, radiographs, computed tomographic scans (3D) and histology. RESULTS: Six weeks after surgery, radiography, computed tomography and histology indicated the different result in healing in the posterolateral fusion using rhBMP-2 compared to ICBG (P < 0.05). Aged BMP-2 group showed significantly higher fusion rates than Aged ICBG group. CONCLUSION: This study demonstrated rhBMP-2 can increase the posterolateral fusion rate and new bone quality in aged rabbitss than autograft, it may take the place of ICBG. But its role is effected by age.
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Envejecimiento , Proteína Morfogenética Ósea 2/farmacología , Proteínas Recombinantes/farmacología , Médula Espinal/efectos de los fármacos , Médula Espinal/cirugía , Fusión Vertebral , Animales , Femenino , Humanos , Palpación , Conejos , Médula Espinal/patología , Médula Espinal/trasplante , Tomografía Computarizada por Rayos X , Trasplante AutólogoRESUMEN
OBJECTIVE: To explore the way to induce mesenchymal stem cells (MSCs) to differentiate into dopaminergic neurons in vitro. METHODS: MSCs were obtained from rat bone marrow, cultured and passaged. MSCs used in this experiment had multipotency, which was indirectly proved by being induced to differentiate into chondrocytes and adipocytes. MSCs were cultured in medium containing 0.5 mmol/L IBMX for 2 days. Then the medium was replaced with induction medium, which contained GDNF, IL-1beta, mesencephalic glial-cell-conditioned medium and flash-frozen mesencephalic membrane fragments. The surface markers of the differentiated neurons, such as NSE, nestin, MAP-2a, b and TH were detected by immunocytochemistry and Western blot after MSCs were cultured in induction medium for 7 days and 15 days. RESULTS: MSCs differentiated into neural progenitors and expressed nestin after MSCs were incubated with medium containing IBMX for 2 d. After the medium was replaced with induction medium containing many inducing agents, MSCs differentiated into neuron-like cells and dopaminergic neuron-like cells and expressed NSE, MAP-2a, b and TH. The percentage of NSE-positive cells, MAP-2a, b-positive cells and TH-positive cells was 30.032 +/- 2.489%, 41.580 +/- 5.101% and 34.958 +/- 5.534%, respectively after MSCs were induced in medium containing GDNF, IL-1beta, mesencephalic glial-cell-conditioned medium and flash-frozen mesencephalic membrane fragments for 15 days. CONCLUSION: MSCs can differentiate into dopaminergic neuron-like cells and are a new cell source for the treatment of neurodegeneration diseases and have a great potential for wide application.