RESUMEN
Comparative genomic hybridization (CGH) has been applied to detect recurrent chromosome alterations in 62 primary gastric carcinomas. Several nonrandom chromosomal changes, including gains of 8q (31 cases, 50%), 20q (29 cases, 47%) with a minimum gain region at 20q11. 2-q12, 13q (21 cases, 34%) with a minimum gain region at 13q22, and 3q (19 cases, 31%) were commonly observed. The regions most frequently lost included: 19p (23 cases, 37%), 17p (21 cases, 33%), and 1p (14 cases, 23%). High copy number gain (DNA sequence amplification) was detected in 6 cases. Amplification of 8q23-q24.2 and 20q11.2-q12 were observed in 3 cases. Gain of 20q and loss of 19p were confirmed by fluorescence in situ hybridization using corresponding bacterial artificial chromosomes (BAC) clones from those regions. The gain and loss of chromosomal regions identified in this study provide candidate regions involved in gastric tumorigenesis.
Asunto(s)
Aberraciones Cromosómicas , Neoplasias Gástricas/genética , Adulto , Anciano , ADN de Neoplasias/genética , Femenino , Humanos , Hibridación Fluorescente in Situ , Masculino , Persona de Mediana Edad , Hibridación de Ácido Nucleico/métodos , Neoplasias Gástricas/patologíaRESUMEN
OBJECTIVE: Research on examination and significance of the auditory brain stem response (ABR) in non-hearing loss tinnitus patients. METHOD: Recording from forehead-mastoid comparing ABR of non-hearing loss tinnitus patients with normal hearing youths. RESULT: In the tinnitus patients, the prolongate latency percentage of wave I was 51.6%; the wave III was 20.9%; wave V was 17.9%. CONCLUSION: Although hearing-loss was not founded in the patients, but the cochlea of some patients had been damaged.
Asunto(s)
Potenciales Evocados Auditivos del Tronco Encefálico , Acúfeno/diagnóstico , Adolescente , Adulto , Enfermedades Cocleares/diagnóstico , Femenino , Humanos , Masculino , Persona de Mediana EdadRESUMEN
OBJECTIVE: Expression of telomerase activity and telomerase RNA component have been reported in various human cancers. In the article, we ascertained whether telomerase activity and hTR changes are characteristic of laryngeal cancer. METHOD: Telomerase activity were examined by telomeric repeat amplification protocol (Trap) and hTR examined by in situ hybridization, in tissue samples from 50 laryngeal cancer, 12 laryngeal polyps, 14 precancerous, 9 normal laryngeal tissue. RESULT: 1. All tissue expressed various levels of hTR. Positive rate of hTR increased with the clinical stage, but no significant correlation was observed, as well as lymph node metastasis. 2. The telomerase activity were detected in samples 39 of the 50 laryngeal cancer(78%). However, in 12 cases of begin lesions only 8.3% (1/12). Telomerase activity was found to have a relationship with the grade of tumor differentiation. Poorly differentiated laryngeal cancer expressed high level telomerase activity. CONCLUSION: Telomerase activity were an early event of laryngeal cancer. It suggests that detection of telomerase activity could be a good diagnostic marker for distinguish cancer from normal cell.