RESUMEN
Oral administration of the orange drink Tampico containing additionally vitamins A (12.7 mg/l), B (1.81 mg/l), C (86.9 mg/l) to FICBAxC57B1/6 mice has been shown to stabilize erythrocytic membranes and reduce chromosomal aberrations in the murine bone marrow cells induced by cyclophosphamide. There are experimental data on the effects of Tampico on the rate of reparative synthesis in peripheral lymphocytes and blood levels of malonic aldehyde in the mice exposed to model psychoemotional stress.
Asunto(s)
Antimutagênicos , Bebidas , Citrus , Animales , Antimutagênicos/farmacología , Ácido Ascórbico/administración & dosificación , Médula Ósea/efectos de los fármacos , Médula Ósea/metabolismo , Células de la Médula Ósea , Células Cultivadas , Aberraciones Cromosómicas , Ciclofosfamida/toxicidad , Membrana Eritrocítica/efectos de los fármacos , Membrana Eritrocítica/metabolismo , Peroxidación de Lípido , Linfocitos/efectos de los fármacos , Linfocitos/metabolismo , Masculino , Malondialdehído/sangre , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Mutagénesis/efectos de los fármacos , Vitamina A/administración & dosificación , Complejo Vitamínico B/administración & dosificaciónAsunto(s)
Contaminación Ambiental/efectos adversos , Enfermedades Genéticas Congénitas/etiología , Contaminantes del Suelo/efectos adversos , Contaminantes del Agua/efectos adversos , ADN/genética , Contaminación Ambiental/análisis , Enfermedades Genéticas Congénitas/diagnóstico , Humanos , Kazajstán , Mutación , Contaminantes del Suelo/análisis , Población Urbana , Contaminantes del Agua/análisisRESUMEN
Bacillus subtilis GTP-cyclohydrolase gene and its deletion derivatives were subcloned in Escherichia coli cells. The position of the gene within the riboflavine operon was defined. The deletion of the 14 kDa fragment from the N-end of GTP-cyclohydrolase gene did not affect the enzyme activity.
Asunto(s)
Bacillus subtilis/enzimología , GTP Ciclohidrolasa/genética , Clonación Molecular , ADN Bacteriano/genética , Electroforesis , Magnesio/metabolismo , Manganeso/metabolismo , Operón , Plásmidos , Biosíntesis de Proteínas , Mapeo Restrictivo , Riboflavina/genéticaRESUMEN
An injection of ionol-type antioxidants stimulates fast oxidation of extramitochondrial NADH in isolated liver mitochondria via an external pathway, which is sensitive to cyanide and resistant to amital and antimycin A. This activation is accompanied by a decrease in cytochrome a and cytochrome (c + C1) levels in liver homogenate. Part of the cytochrome c pool can be removed by washing the antioxidant-treated mitochondria. It is assumed that the injection of the antioxidants leads to desorption of the cytochrome c bound to the inner membrane into the intermembrane space of mitochondria. This cytochrome acts as a linking agent between NADH cytochrome c reductase of the outer mitochondrial membrane and cytochrome c oxidase of the inner membrane. The Li-derivative of ionol (5 x 10(-4) and 10(-3) M) decreases the transmembrane potential of the submitochondrial particles from bovine heart by 15-20%.