RESUMEN
UNLABELLED: Acetate is preferentially transported into and metabolized by astrocytes, rather than synaptosomes or neurons, and labeled acetate is used as a glial reporter molecule to assess glial metabolism and glial-neuronal interactions. Because monocarboxylic acid transporter specificity might confer a phenotype to help localize, detect, and characterize brain tumors of glial origin, use of [2-(14)C]acetate and [(14)C]deoxyglucose (a glucose analog metabolized by all brain cells) was compared in rat and human brain tumors. METHODS: Cultured C6 glioma or U-373 glioblastoma/astrocytoma tumor cells were injected into the caudate nucleus of anesthetized CDF Fisher rats; 2--3 wk later, an intravenous pulse of [2-(14)C]acetate or [(14)C]deoxyglucose was given, and timed blood samples were drawn during the 5- or 45-min experiment, respectively. Local (14)C levels in the brain were assayed by quantitative autoradiography, and acetate uptake or glucose use was calculated. Uptake and metabolism of the [(14)C]acetate was also assayed in C6 glioma and human surgical tumor samples in vitro. RESULTS: [(14)C]Acetate uptake into rat brain C6 tumors was 9.9 +/- 2.1 mL/100 g/min, compared with 3.9 +/- 1.0 mL/100 g/min in contralateral tissue (n = 6; P < 0.001), and was much higher than that into other brain structures (e.g., 5:1 for white matter and 2:1 for cortical gray matter). Glucose use in C6 tumors was 111 +/- 34 micromol/100 g/min, versus 81 +/- 5 micromol/100 g/min in contralateral tissue (n = 6; P = 0.08); no left-right differences in glucose use or acetate uptake were seen in other brain structures. The tumor-to-contralateral-tissue ratio for acetate (2.3 +/- 0.3) exceeded that for deoxyglucose (1.4 +/- 0.5) (P < 0.05), indicating that acetate is a sensitive C6 glioma marker. [(14)C]Acetate uptake also demarcated a few 3-wk-old C6 tumors that had unlabeled necrotic cores. U-373 tumors were smaller than C6 tumors in rat brain and were detected equally well with [(14)C]acetate and [(14)C]deoxyglucose. In vitro uptake of [(14)C]acetate into human glioblastoma or meningioma tumors was higher than uptake into pituitary adenoma. Rat C6 and human tumors with high uptake metabolized acetate to acidic compounds and amino acids. CONCLUSION: Tumor imaging with radiolabeled acetate can help to localize and classify brain tumors. Transporter and metabolic substrate specificity are traits that can be exploited further for in vivo imaging of brain glial tumors.
Asunto(s)
Ácido Acético , Neoplasias Encefálicas/diagnóstico por imagen , Glioma/diagnóstico por imagen , Meningioma/diagnóstico por imagen , Radiofármacos , Animales , Biotransformación , Encéfalo/metabolismo , Radioisótopos de Carbono , Humanos , Trasplante de Neoplasias , Cintigrafía , Radiofármacos/farmacocinética , Ratas , Trasplante Heterólogo , Células Tumorales CultivadasRESUMEN
Murine monoclonal antibody 206 (MAb mu206) binds to gH, the varicella-zoster virus (VZV) fusogen, neutralizing the virus in vitro in the absence of complement and inhibiting cell-to-cell spread and egress of VZV in cultured cells. We have humanized this antibody to generate MAb hu206 by complementarity determining region grafting. MAb hu206 retained binding and in vitro neutralizing activity, as well as cross-reactivity with ten different VZV strains. Single-chain antibody fragments (scAb) derived from MAb hu206 were produced in Escherichia coli. These scAb retained the binding properties of the whole antibody. However, monomeric scAb exhibited markedly reduced neutralizing activity compared to the bivalent parental MAb hu206. Shortening the peptide linker joining the V(H) to the V(kappa) domain from 14 to 5 or even 0 residues encouraged multimerization and increased neutralizing efficacy. The fact that Fab fragments enzymatically generated from whole MAb hu206 lost their neutralizing potency lent support to the proposal that valency is important for VZV neutralization at this epitope.
Asunto(s)
Anticuerpos Antivirales/inmunología , Herpesvirus Humano 3/inmunología , Animales , Anticuerpos Monoclonales/inmunología , Anticuerpos Antivirales/biosíntesis , Anticuerpos Antivirales/genética , Línea Celular , Relación Dosis-Respuesta Inmunológica , Escherichia coli/genética , Vectores Genéticos , Glicoproteínas/inmunología , Herpes Zóster/inmunología , Herpesvirus Humano 3/química , Humanos , Fragmentos Fab de Inmunoglobulinas/inmunología , Ratones , Pruebas de Neutralización , Ingeniería de Proteínas , Proteínas del Envoltorio Viral/inmunologíaRESUMEN
Prostaglandins are generally considered pro-inflammatory molecules that contribute to the pathology associated with a variety of immune-mediated diseases including multiple sclerosis. However, recently it has been demonstrated that specific cyclopentone prostaglandin metabolites including 15-deoxy-Delta(12,14) prostaglandin J2 (15d-PGJ2) are capable of repressing the production of pro-inflammatory molecules by cells of the monocyte/macrophage lineage. Activated microglia produce nitric oxide (NO) and TNF-alpha, molecules which can be toxic to cells including oligodendrocytes, thus potentially contributing to the pathology associated with multiple sclerosis. The current study demonstrates that 15d-PGJ2 inhibits lipopolysachharide (LPS) induction of NO and TNF-alpha production by rat primary microglia and mouse N9 microglial cells. 15d-PGJ2 also inhibits NO production by microglial cells activated in response to IFN-gamma and TNF-alpha, cytokines believed to be important modulators of multiple sclerosis. IL-12 plays a critical role in stimulating the production of Th1 cells, which are believed to contribute to the pathology associated with multiple sclerosis. The current studies demonstrate that 15d-PGJ2 represses the production of IL-12 by microglial cells. Collectively, these studies demonstrate that the prostaglandin metabolite 15d-PGJ2 represses microglial production of potentially cytotoxic molecules, as well as molecules capable of altering T-cell phenotype. These in vitro studies suggest the possibility that the prostaglandin 15d-PGJ2 may modulate inflammatory diseases including multiple sclerosis.
Asunto(s)
Interleucina-12/antagonistas & inhibidores , Microglía/efectos de los fármacos , Óxido Nítrico/antagonistas & inhibidores , Prostaglandina D2/farmacología , Factor de Necrosis Tumoral alfa/antagonistas & inhibidores , Animales , Western Blotting , Células Cultivadas , Molécula 1 de Adhesión Intercelular/metabolismo , Interferón gamma/farmacología , Interleucina-12/biosíntesis , Lipopolisacáridos/farmacología , Microglía/citología , Microglía/metabolismo , Óxido Nítrico/biosíntesis , Óxido Nítrico Sintasa/metabolismo , Óxido Nítrico Sintasa de Tipo II , Prostaglandina D2/análogos & derivados , Ratas , Factor de Necrosis Tumoral alfa/biosíntesis , Factor de Necrosis Tumoral alfa/farmacologíaRESUMEN
Multiple sclerosis occurs more commonly in females than males. However, the mechanisms resulting in gender differences in multiple sclerosis are unknown. Activated microglia are believed to contribute to multiple sclerosis pathology, perhaps in part due to production of nitric oxide (NO) and TNF-alpha, molecules which can be toxic to cells including oligodendrocytes. The current study demonstrates that the female sex steroids estriol, beta-estradiol and progesterone inhibit lipopolysaccharide (LPS) induction of nitric oxide (NO) production by primary rat microglia and by the mouse N9 microglial cell line. These hormones act by inhibiting the production of inducible nitric oxide synthase (iNOS) which catalyses the synthesis of NO. Estriol likely inhibits iNOS gene expression since the hormone blocks LPS induction of iNOS RNA levels. The pro-inflammatory cytokines IFN-gamma and TNF-alpha are believed to be important modulators of multiple sclerosis. Here, we demonstrate that estrogens and progesterone also inhibit NO production by microglial cells activated in response to these cytokines. Activated microglia elicit TNF-alpha in addition to NO and we further demonstrate that estrogens and progesterone repress TNF-alpha production by these cells. Finally, estriol and progesterone, at concentrations consistent with late pregnancy, inhibit NO and TNF-alpha production by activated microglia, suggesting that hormone inhibition of microglial cell activation may contribute to the decreased severity of multiple sclerosis symptoms commonly associated with pregnancy.
Asunto(s)
Estradiol/farmacología , Estriol/farmacología , Microglía/inmunología , Microglía/metabolismo , Progesterona/farmacología , Animales , Células Cultivadas , Femenino , Expresión Génica/efectos de los fármacos , Expresión Génica/inmunología , Interferón gamma/metabolismo , Lipopolisacáridos/farmacología , Microglía/citología , Esclerosis Múltiple/tratamiento farmacológico , Esclerosis Múltiple/inmunología , Esclerosis Múltiple/metabolismo , Óxido Nítrico/metabolismo , Óxido Nítrico Sintasa/genética , Óxido Nítrico Sintasa/metabolismo , Óxido Nítrico Sintasa de Tipo II , Embarazo , Complicaciones del Embarazo/inmunología , ARN Mensajero/metabolismo , Ratas , Factor de Necrosis Tumoral alfa/metabolismoRESUMEN
Dehydroepiandrosterone (DHEA) is a steroid that circulates in abundance in the form of a sulfated reserve (DHEA-S). The levels of DHEA decline with age and further in age-related neuropathologies, including Alzheimer disease. Because of their reported anti-inflammatory effects, we tested the actions of these compounds on microglia. At concentrations of 3(-9) to 1(-6) M, DHEA and DHEA-S inhibited the production of nitrite and morphological changes stimulated by lipopolysaccharide. DHEA and DHEA-S also inhibited LPS induction of iNOS protein, but neither inhibited LPS-induced iNOS mRNA or the activation of NF-kappaB. These data suggest that the hormone regulates nitrite production through a post-transcriptional mechanism. Interestingly, microglial nitrite production in response to a secreted form of the beta-amyloid precursor protein (sAPP) was unaffected by DHEA. Another Alzheimer-related factor, amyloid beta-peptide, also stimulated microglial nitrite production but in a manner dependent on the co-stimulus interferon-gamma. DHEA was found to inhibit only the interferon-gamma component of the microglial response. These data add to a growing body of evidence for differences in the profiles of mononuclear phagocytes activated by distinct stimuli.
Asunto(s)
Enfermedad de Alzheimer/enzimología , Deshidroepiandrosterona/metabolismo , Deshidroepiandrosterona/farmacología , Encefalitis/enzimología , Microglía/efectos de los fármacos , Óxido Nítrico Sintasa/efectos de los fármacos , Óxido Nítrico/biosíntesis , Enfermedad de Alzheimer/genética , Enfermedad de Alzheimer/fisiopatología , Animales , Animales Recién Nacidos , Células Cultivadas , Sulfato de Deshidroepiandrosterona/metabolismo , Sulfato de Deshidroepiandrosterona/farmacología , Encefalitis/genética , Encefalitis/fisiopatología , Microglía/metabolismo , Óxido Nítrico Sintasa/metabolismo , Nitritos/metabolismo , Ratas , Ratas Sprague-Dawley , Transcripción Genética/efectos de los fármacos , Transcripción Genética/fisiologíaRESUMEN
Cortisol is a steroid hormone produced in response to stress. This glucocorticoid can be toxic to neurons, and thus may be important in neurodegenerative diseases including Alzheimer's disease. Activated microglia produce molecules including nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha) which can also be toxic to neurons. The current study was designed to determine the effect of cortisol upon the activation of primary cultured microglia and transformed N9 microglial cells. The studies indicate that cortisol represses lipopolysaccharide (LPS) induction of nitric oxide production in these microglial cells. The hormone acts by inhibiting the production of inducible nitric oxide synthase (iNOS) which catalyses the synthesis of NO. Cortisol likely acts by blocking transcription of iNOS gene expression since the hormone represses LPS induction of iNOS RNA levels in these cells. Activated microglia produce increased TNF-alpha, in addition to increased NO. The current studies demonstrate that cortisol inhibits release of TNF-alpha from LPS-treated microglial cells. Collectively, these data suggest that although cortisol may be directly toxic to neurons, the hormone may indirectly protect neurons by blocking the production of cytotoxic molecules by microglia.
Asunto(s)
Hidrocortisona/farmacología , Microglía/efectos de los fármacos , Microglía/metabolismo , Animales , Animales Recién Nacidos , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Relación Dosis-Respuesta a Droga , Ensayo de Inmunoadsorción Enzimática , Expresión Génica/efectos de los fármacos , Hidrocortisona/metabolismo , Lipopolisacáridos/farmacología , Microglía/citología , Óxido Nítrico/biosíntesis , Óxido Nítrico Sintasa/biosíntesis , Óxido Nítrico Sintasa/genética , Óxido Nítrico Sintasa de Tipo II , ARN Mensajero/biosíntesis , Ratas , Factor de Necrosis Tumoral alfa/biosíntesisRESUMEN
A peptidoglycan-associated lipoprotein (PAL) fused to an antibody fragment (scFv) specific to the herbicide and environmental pollutant atrazine, has been successfully targeted to the cell surface of Escherichia coli. Anti-atrazine binding could be observed via an atrazine-alkaline phosphatase conjugate. Cells containing the PAL fusion grew with little cellular toxicity when compared with the control. In contrast, expression of anti-atrazine antibody fragments alone caused the cells to lyse after 4 h. The surface display of anti-pollutant antibodies may have a future role in the bioremediation of contaminated water or the development of pollutant-specific, whole-cell biosensors.
Asunto(s)
Atrazina/inmunología , Escherichia coli/inmunología , Fragmentos de Inmunoglobulinas/biosíntesis , Contaminantes del Agua/inmunología , Clonación Molecular , Escherichia coli/genética , Vectores Genéticos , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/genéticaRESUMEN
N9 microglial cells were used as a model to examine the effect of cholesterol oxides on central nervous system microglia. Results indicated that 25-OH-cholesterol was the most cytotoxic agent among the cholesterol oxides tested. During the process of cell death, this agent caused prominent nuclei condensation and significant DNA fragmentation, a phenomenon association with programmed cell death. Cholesterol oxides were able to potentiate the bacterial lipopolysaccharide (LPS)-induced nitric oxide production to various degrees. Consistent with this finding, Northern blot analysis indicated that 25-OH-cholesterol potentiated the LPS-induced nitric oxide synthase RNA levels. The cytotoxicity of 25-OH-cholesterol could be prevented by methyl-beta-cyclodextrin, a glucose polymer known to cause cholesterol oxide efflux from cells. While much attention has been focused on the cytotoxicity of cholesterol oxides on immune cells within the blood, including lymphocytes and macrophages, the results from this study indicated for the first time that these agents are toxic to microglial cells derived from the central nervous system.
Asunto(s)
Apoptosis/efectos de los fármacos , Hidroxicolesteroles/toxicidad , Microglía/efectos de los fármacos , beta-Ciclodextrinas , Animales , Línea Celular , Ciclodextrinas/farmacología , Fragmentación del ADN/efectos de los fármacos , Sinergismo Farmacológico , Hidroxicolesteroles/administración & dosificación , Hidroxicolesteroles/antagonistas & inhibidores , Lipopolisacáridos/administración & dosificación , Lipopolisacáridos/toxicidad , Ratones , Microglía/metabolismo , Microglía/patología , Óxido Nítrico/biosíntesis , Óxido Nítrico Sintasa/genética , ARN Mensajero/biosíntesis , ARN Mensajero/genéticaAsunto(s)
Cromosomas Humanos Par 7/genética , Proteínas de Unión al ADN , Proteínas del Tejido Nervioso/genética , Factores de Transcripción/genética , Dedos de Zinc/genética , Secuencia de Aminoácidos , Secuencia de Bases , Mapeo Cromosómico , Clonación Molecular , ADN Complementario/genética , Expresión Génica , Humanos , Datos de Secuencia Molecular , Homología de Secuencia de AminoácidoAsunto(s)
Mapeo Cromosómico , Cromosomas Humanos Par 19 , Regulación del Desarrollo de la Expresión Génica , Proteínas del Tejido Nervioso/genética , Factores de Transcripción , Dedos de Zinc/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Clonación Molecular , ADN Complementario , Evolución Molecular , Hipocampo , Humanos , Datos de Secuencia Molecular , Homología de Secuencia de AminoácidoRESUMEN
We report the cloning and characterization of a novel cDNA termed C2H2-546 which encodes a C2H2-type zinc finger protein. C2H2-546 RNA is expressed in the HTLV-1 infected T cells examined which were derived from HAM-TSP patients, but not in T cells derived from ATL patients. The C2H2-546 gene is conserved in humans and primates and maps to chromosome 10q11.2, a site associated with a variety of cancers. Thus, C2H2-546 is a candidate regulatory molecule important in the formation of these tumors, and may serve as an important marker to distinguish HTLV-1 infected ATL versus HAM-TSP T cell lineages.
Asunto(s)
Cromosomas Humanos Par 10/genética , Regulación Viral de la Expresión Génica , Genes , Infecciones por HTLV-I/genética , Virus Linfotrópico T Tipo 1 Humano/fisiología , Proteínas de Neoplasias/genética , Linfocitos T/metabolismo , Dedos de Zinc/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Cromosomas Artificiales de Levadura , ADN Complementario/genética , Drosophila melanogaster/genética , Humanos , Factores de Transcripción de Tipo Kruppel , Leucemia-Linfoma de Células T del Adulto/genética , Leucemia-Linfoma de Células T del Adulto/patología , Mamíferos/genética , Datos de Secuencia Molecular , Proteínas de Neoplasias/biosíntesis , Proteínas Nucleares , Paraparesia Espástica Tropical/genética , Paraparesia Espástica Tropical/patología , Reacción en Cadena de la Polimerasa , Saccharomyces cerevisiae/genética , Especificidad de la Especie , Linfocitos T/virología , Factores de Transcripción , Células Tumorales CultivadasRESUMEN
We describe a novel human zinc finger cNDA. C2H2-171. This cDNA represents an mRNA which encodes a protein of 484 amino acids and a calculated molecular weight of 54 kD. Four zinc finger-like domains are found in the C-terminal end of the protein. At the N-terminus, C2H2-171 contains a POZ/tramtrack-like domain similar to that found in the tumor associated zinc finger proteins LAZ-3/BCL-6 and PLZ-F, as well as in non-zinc finger proteins. C2H2-171 RNA is preferentially expressed in the brain, and increases during the course of murine development, with maximal expression in the adult. C2H2-171 RNA is differentially expressed in brain regions, with the highest level of expression in the cerebellum. C2H2-171 RNA was expressed at high levels in primary cerebellar granule cell neurons compared to astrocytes. The gene encoding C2H2-171 is highly conserved in vertebrates, and maps to the terminus of human chromosome 1 (1q44-ter). This chromosomal location is associated with a number of cytogenetic aberrations including those involving brain developmental anomalies and tumorigenesis. These data suggest that C2H2-171 may play an important role in vertebrate brain development and function.
Asunto(s)
Encéfalo/metabolismo , ADN Complementario/análisis , Proteínas del Tejido Nervioso/genética , Estructura Terciaria de Proteína , ARN Mensajero/genética , Dedos de Zinc , Secuencia de Aminoácidos , Secuencia de Bases , Encéfalo/crecimiento & desarrollo , Neoplasias Encefálicas/genética , Mapeo Cromosómico , Cromosomas Humanos Par 1 , Secuencia Conservada , Femenino , Código Genético , Humanos , Datos de Secuencia Molecular , Neuronas/metabolismoRESUMEN
Major histocompatibility (MHC) class I gene expression is synergistically induced by the cytokines TNF-alpha and IFN-gamma. However, the mechanism that results in synergistic activation of these genes has remained unclear. We demonstrated here that TNF-alpha induced binding of NF kappa B p50 and p65 to the NF kappa B-like element of the MHC class I promoter termed region I and IFN-gamma induced binding of IRF-1 to the adjacent interferon consensus sequence (ICS). We further demonstrated that NF kappa B and IRF-1 physically interacted with each other and cooperatively induced MHC class I gene expression when cotransfected into CHP-126 neuroblastomas. These results provide a molecular mechanism by which TNF-alpha and IFN-gamma synergistically induce the expression of a variety of genes involved in immune responses, including MHC class I.
Asunto(s)
Secuencia de Consenso , Proteínas de Unión al ADN/metabolismo , Regulación de la Expresión Génica/efectos de los fármacos , Genes MHC Clase I , FN-kappa B/metabolismo , Fosfoproteínas/metabolismo , Factores de Transcripción/metabolismo , Secuencia de Bases , Sinergismo Farmacológico , Vectores Genéticos , Humanos , Factor 1 Regulador del Interferón , Interferón gamma/farmacología , Datos de Secuencia Molecular , Regiones Promotoras Genéticas , Estimulación Química , Células Tumorales Cultivadas , Factor de Necrosis Tumoral alfa/farmacologíaRESUMEN
Most neural cells constitutively lack major histocompatibility complex (MHC) class I and beta 2-microglobulin gene expression. Cytokines and viruses may, however, induce expression of these genes in some neural cells, and this correlates with factor binding to the NF-kappa B and interferon stimulated response elements of these genes. Here, we demonstrate that NF-kappa B is capable of inducing MHC class I and beta 2-microglobulin gene expression when transiently co-transfected into CHP-126 neuroblastomas, and that IRF-2 represses this induction. Interferon regulatory factor-2 (IRF-2) repression of MHC class I and beta 2-microglobulin gene expression in CHP-126 neuroblastomas may demonstrate a mechanism by which virus persists in neural cells. We show here that IRF-2 physically interacts in vitro with NF-kappa B. This interaction may contribute to the repression of the expression of these genes. Our demonstration that IRF family members, in addition to IRF-2, physically interact in vitro with NF-kappa B (p50 and p65), provides a general mechanism by which these transcription factors may, in concert, regulate the expression of a variety of genes involved in immune responses in the brain.
Asunto(s)
Proteínas de Unión al ADN/inmunología , Antígenos de Histocompatibilidad Clase I/inmunología , FN-kappa B/inmunología , Proteínas Represoras/inmunología , Factores de Transcripción , Microglobulina beta-2/genética , Secuencia de Bases , Proteínas de Unión al ADN/biosíntesis , Proteínas de Unión al ADN/metabolismo , Regulación de la Expresión Génica/inmunología , Antígenos de Histocompatibilidad Clase I/metabolismo , Humanos , Factor 2 Regulador del Interferón , Datos de Secuencia Molecular , FN-kappa B/antagonistas & inhibidores , FN-kappa B/metabolismo , Neuroblastoma/inmunología , Regiones Promotoras Genéticas/inmunología , Proteínas Represoras/metabolismo , Transfección , Células Tumorales Cultivadas/inmunología , Microglobulina beta-2/inmunologíaRESUMEN
Adult neurons normally lack the expression of MHC class I molecules, which has implications on virus clearance from the central nervous system. The author previously demonstrated that HLA class I up-regulation in measles virus (MV)-infected glial cells is primarily mediated by IFN-beta. In contrast, this study demonstrates that MV-infection of the neuronal cell lines IMR-32 and CHP-126 fails to up-regulate HLA class I expression, which was associated with an inability of MV to induce IFN-beta in the neuronal cell lines. However, treatment with IFN-beta on coculture of the IMR-32 neuronal cell line with MV-infected glioma cells resulted in the up-regulation of HLA class I on the former, which could be neutralized by anti-IFN-beta Ab. The inability of MV to up-regulate HLA class I expression on the neuronal cell line IMR-32 was not virus specific because similar findings were observed with mumps virus or stimulation with the synthetic dsRNA polyinosinic polycytidylic acid (PIPC). Induction of IFN-beta gene expression by virus requires binding of NF-kappa B to the positive regulatory domain II element of the IFN-beta promoter. Our studies indicate that MV, TNF-alpha, or PIPC induces NF-kappa B (p50 and p65 subunits) binding to positive regulatory domain II in the glioma cell line. In contrast, such activity was induced by TNF-alpha but not MV or PIPC in the neuronal cell line IMR-32. This indicated that HLA class I expression is differentially regulated in glial and neuronal cell lines in response to MV, which correlates with differential binding of NF-kappa B to the IFN-beta promoter and induction of IFN-beta gene expression.
Asunto(s)
Antígenos de Histocompatibilidad Clase I/biosíntesis , Interferón beta/biosíntesis , Virus del Sarampión/fisiología , Virus de la Parotiditis/fisiología , Neuroglía/inmunología , Neuronas/inmunología , Secuencia de Bases , Humanos , Interferón beta/genética , Datos de Secuencia Molecular , FN-kappa B/metabolismo , Poli I-C/farmacología , ARN Mensajero/análisis , Células Tumorales Cultivadas , Regulación hacia ArribaRESUMEN
We report here the nucleotide sequence of a clone, C2H2-34.10, isolated from a human brain cDNA library using degenerate oligodeoxyribonucleotide hybridization. C2H2-34.10 has extensive homology to the Xenopus laevis 5S DNA/RNA-binding protein, TFIIIA. The deduced amino acid (aa) sequence of the human clone gives a protein of 363 aa with identity to TFIIIA from both X. laevis (57%) and Rana pipiens (59%). This human clone contains nine C2H2-type zinc fingers like frog TFIIIA. Northern blot analysis indicates that the C2H2-34.10 RNA is expressed in human ovary, as well as human neuronal cell lines.
Asunto(s)
Proteínas de Unión al ADN/genética , Factores de Transcripción/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Northern Blotting , Encéfalo/fisiología , ADN Complementario/genética , Biblioteca de Genes , Humanos , Datos de Secuencia Molecular , Rana pipiens/genética , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido , Distribución Tisular , Factor de Transcripción TFIIIA , Xenopus laevis/genéticaRESUMEN
C2H2-type zinc finger genes comprise one of the largest gene families in the human genome. These proteins are involved in genetic regulation and development and are quite conserved throughout evolution. The finger domains commonly contain the small linker peptide TGEKP between some finger units. Here, we report the isolation of 133 human zinc finger cDNAs, of which 118 are novel. These clones were isolated from human brain cDNA libraries using oligonucleotide hybridization followed by expressed sequence tag (EST) analysis, sequencing from the conserved linker region using degenerate oligonucleotide primers. This directed partial sequencing approach to cDNA isolation and characterization, signature sequencing, combines the speed of EST automatic sequencing with the focus of specific cDNA family analysis. Signature sequencing minimizes the generation of less informative random EST sequences and provides a unique relative position for sequence comparison. We also show that there is an even distribution of these RNAs from this brain cDNA library, and that these cDNAs contain N-terminal domains found in other zinc finger genes. This rapid focused sequencing approach should be applicable to any family of cDNAs containing short conserved signature peptide sequences.
Asunto(s)
Encéfalo/metabolismo , Dedos de Zinc/genética , Secuencia de Aminoácidos , Secuencia de Bases , Clonación Molecular , ADN Complementario , Humanos , Datos de Secuencia Molecular , Homología de Secuencia de AminoácidoRESUMEN
In vitro, HIV-1 infection of human fetal glial cells initiates a noncytopathic, productive infection that results in a long-term persistence during which the viral genome remains latent. The cytokines tumor necrosis factor alpha (TNF-alpha) and interleukin 1 beta (IL-1 beta) reactivate HIV-1 gene expression in these cells, leading to production of infectious virus. Here we show that treatment of human fetal glial cells with TNF-alpha and IL-1 beta increase expression of the reporter gene chloramphenicol acetyltransferase (CAT) when placed under the control of the HIV-1 5' LTR. We also show that treatment of human fetal glial cells with TNF-alpha leads to increased binding of the nuclear transcription factor NF-kappa B (p50/p65) to a consensus kappa B-binding site present in the HIV-1 5'LTR. Our results suggest that TNF-alpha stimulation of HIV-1 gene expression in primary cultures of human fetal glial cells is mediated by an increase in binding of NF-kappa B (p50/p65) to the HIV-1 LTR. This is the first report documenting NF-kappa B-binding activity in primary cultures of human fetal glial cells.
Asunto(s)
Expresión Génica/efectos de los fármacos , VIH-1/fisiología , FN-kappa B/biosíntesis , Neuroglía/metabolismo , Factor de Necrosis Tumoral alfa/farmacología , Secuencia de Bases , Sitios de Unión , Encéfalo/metabolismo , Núcleo Celular/metabolismo , Citocinas/farmacología , Feto , Genoma Viral , Duplicado del Terminal Largo de VIH , VIH-1/efectos de los fármacos , VIH-1/genética , Humanos , Datos de Secuencia Molecular , FN-kappa B/metabolismo , Subunidad p50 de NF-kappa B , Neuroglía/efectos de los fármacos , Neuroglía/virología , Sondas de Oligonucleótidos , Factor de Transcripción ReIA , Transfección , Latencia del VirusRESUMEN
Expression of the beta 2-microglobulin (beta 2-m) and major histocompatibility complex (MHC) class I genes is coordinately regulated. By ligation-mediated polymerase chain reaction, we have analyzed in vivo factor binding to the promoter region of the murine beta 2-m gene. In adult spleen, in which beta 2-m is expressed, strong protection was found in three elements. Two of these elements, the beta 2-m NF-kappa B binding site and the interferon consensus sequence, are homologous to the regulatory elements of the MHC class I genes and were also found to be protected in spleen. A third protected element, PAM, identified in this work, is unique to the beta 2-m gene. None of the elements showed protection in brain tissue, in which neither the beta 2-m nor the MHC class I gene is expressed. In vivo footprinting was also performed with F9 embryonal carcinoma cells, in which expression of the beta 2-m and MHC class I genes is induced at a low level only upon stimulation with retinoic acid (RA). No in vivo protection was detected before and after RA treatment of F9 cells, indicating that RA induction of beta 2-m (and MHC class I) expression occurs without detectable in vivo factor occupancy, whereas EL4 T lymphocytes expressing beta 2-m at a high level exhibited strong protection similar to that in spleen. Despite the lack of in vivo occupancy, the nuclear factors specific for each of the three elements were present in brain tissue and F9 cells as well as in spleen tissue and EL4 cells. We show that PAM, an element identified by its in vivo protection, binds nuclear factors ranging from 40 to 50 kDa in size and is capable of enhancing transcription of a reporter in F9 and other cells. Taken together, these results indicate that in vivo factor occupancy for the beta 2-m and MHC class I promoters is coordinated and occurs through a mechanism other than mere expression of relevant factors.
Asunto(s)
ADN/química , Regiones Promotoras Genéticas , ARN Mensajero/biosíntesis , Secuencias Reguladoras de Ácidos Nucleicos , Microglobulina beta-2/genética , Animales , Secuencia de Bases , Carcinoma Embrionario , Núcleo Celular/metabolismo , ADN/metabolismo , Cartilla de ADN , Proteínas de Unión al ADN/aislamiento & purificación , Proteínas de Unión al ADN/metabolismo , Femenino , Regulación de la Expresión Génica , Genes MHC Clase I , Ratones , Ratones Endogámicos C57BL , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa/métodos , Homología de Secuencia de Ácido Nucleico , Células Tumorales CultivadasRESUMEN
Retinoic acid (RA) treatment of human embryonal carcinoma (EC) NTera-2 (NT2) cells induces expression of major histocompatibility complex (MHC) class I and beta-2 microglobulin surface molecules. We found that this induction was accompanied by increased levels of MHC class I mRNA, which was attributable to the activation of the two conserved upstream enhancers, region I (NF-kappa B like) and region II. This activation coincided with the induction of nuclear factor binding activities specific for the two enhancers. Region I binding activity was not present in undifferentiated NT2 cells, but binding of an NF-kappa B heterodimer, p50-p65, was induced following RA treatment. The p50-p65 heterodimer was produced as a result of de novo induction of p50 and p65 mRNAs. Region II binding activity was present in undifferentiated cells at low levels but was greatly augmented by RA treatment because of activation of a nuclear hormone receptor heterodimer composed of the retinoid X receptor (RXR beta) and the RA receptor (RAR beta). The RXR beta-RAR beta heterodimer also bound RA responsive elements present in other genes which are likely to be involved in RA triggering of EC cell differentiation. Furthermore, transfection of p50 and p65 into undifferentiated NT2 cells synergistically activated region I-dependent MHC class I reporter activity. A similar increase in MHC class I reporter activity was demonstrated by cotransfection of RXR beta and RAR beta. These data show that following RA treatment, heterodimers of two transcription factor families are induced to bind to the MHC enhancers, which at least partly accounts for RA induction of MHC class I expression in NT2 EC cells.