RESUMEN
AIMS: To evaluate the behaviour of Shiga toxin-producing Escherichia coli (STEC) O26 strains inoculated in manure-amended soils under in vitro conditions. METHODS AND RESULTS: Four green fluorescent protein (GFP)-labelled STEC O26 strains were inoculated in duplicate (at 10(6) CFU g(-1)) in three different manure-amended soil types, including two loam soils (A and B) and one clay loam soil (C), and two incubation temperatures (4 and 20 degrees C) were tested. STEC counts and soil physical parameters were periodically monitored. STEC O26 cells were able to persist during extended periods in soil even in the presence of low moisture levels, i.e. less than 0 x 08 g H2O g(-1) dry soil. At 4 and 20 degrees C, STEC could be detected in soil A for 288 and 196 days, respectively, and in soils B and C for at least 365 days postinoculation at both temperatures. The ambient temperature (i.e. 20 degrees C) was significantly associated with the highest STEC count decline in all soils tested. CONCLUSIONS: The temperature and soil properties appear to be contributory factors affecting the long-term survival of STEC O26 in manure-amended soils. SIGNIFICANCE AND IMPACT OF THE STUDY: This study provides useful information regarding the ecology of STEC O26 in manure-amended soils and may have implications for land and waste management.
Asunto(s)
Crianza de Animales Domésticos , Estiércol , Escherichia coli Shiga-Toxigénica/fisiología , Microbiología del Suelo , Administración de Residuos/métodos , Silicatos de Aluminio , Animales , Bovinos , Arcilla , Recuento de Colonia Microbiana , Monitoreo del Ambiente/métodos , Marcadores Genéticos , Proteínas Fluorescentes Verdes/genética , Humedad , Concentración de Iones de Hidrógeno , Viabilidad Microbiana , Escherichia coli Shiga-Toxigénica/genética , Suelo , TemperaturaRESUMEN
AIMS: The main objective of this study was to evaluate the growth and survival of Shiga toxin-producing Escherichia coli (STEC) O26 in cow slurry; this serogroup is regarded as an important cause of STEC-associated diseases. METHODS AND RESULTS: Four STEC were examined by polymerase chain reaction (PCR) to determine whether they harbour key virulence determinants and also by pulsed-field gel electrophoresis (PFGE) to obtain overview fingerprints of their genomes. They were transformed with the pGFPuv plasmid and were separately inoculated at a level of 10(6) CFU ml(-1) in 15 l of cow slurry. All STEC O26 strains could be detected for at least 3 months in cow slurry without any genetic changes. The moisture content of the slurry decreased over time to reach a final value of 75% while the pH increased from 8.5 to 9.5 units during the last 50 days. CONCLUSION: STEC O26 strains were able to survive in cow slurry for an extended period. SIGNIFICANCE AND IMPACT OF THE STUDY: Long-term storage of waste slurry should be required to reduce the pathogen load and to limit environmental contamination by STEC O26.