RESUMEN
Virus free supernatants (VFS) obtained by ultracentrifugation of homogenates of African swine fever (ASF) virus infected cultures inhibited the proliferative response and the expression in peripheral blood mononuclear cells of two activation molecules, the IL-2 receptor (IL-2R) and the swine MHC class II antigens (SLA II), induced by several stimuli (lectins, PMA plus the calcium ionophore A23187 or specific antigen). This inhibition was time dependent: no effect was seen on IL-2R expression when VFS was added after 48 h, when the expression of this molecule reached its maximum. However at this time the proliferative response was still inhibited. The presence of VFS in the cultures was necessary to inhibit both the IL-2R expression and the proliferation of cells. In these conditions the addition of exogenous IL-2 to the cultures failed to restore the IL-2R expression and the proliferation shown by control stimulated cells. Furthermore, the IL-2 activity found in supernatants from cell cultures stimulated with Con A in the presence of VFS was even higher than in cultures stimulated without VFS. The inhibition observed suggests an important impairment of host immunocompetence in ASF infected swine.
Asunto(s)
Virus de la Fiebre Porcina Africana/inmunología , Antígenos de Histocompatibilidad Clase II/biosíntesis , Leucocitos Mononucleares/inmunología , Receptores de Interleucina-2/biosíntesis , Animales , Línea Celular , Medios de Cultivo Condicionados , Interleucina-2/biosíntesis , Interleucina-2/farmacología , Receptores de Interleucina-2/antagonistas & inhibidores , Porcinos , Factores de TiempoRESUMEN
Pulmonary carcinoid tumors presenting as peripheral or coin lesions are rare and radiologically may resemble other primary or metastatic neoplasms in the lung. This study consisted of the cytologic evaluation of fine-needle aspirates from five peripheral carcinoid tumors of the lung with particular reference to the differences between the cytologic manifestations of this neoplasm and of small-cell carcinoma and well-differentiated adenocarcinoma. Aspirates of typical carcinoid tumors are characterized by isolated cells and loose aggregates of cells; spindle- and oval-shaped cells of uniform size with scanty, pale eosinophilic cytoplasm; and nuclei with evenly dispersed finely granular chromatin and usually prominent single micronucleoli with occasional macronucleoli. In contrast, the cells of small-cell carcinoma are pleomorphic and arranged in noncohesive loose aggregates, their cytoplasm is scanty, and they show nuclear molding. Their nuclei are hyperchromatic with fine to coarsely granular chromatin. The cells of well-differentiated adenocarcinoma are arranged in three-dimensional clusters or loose aggregates of relatively uniform cells with a columnar configuration. The nuclei are uniformly round and hyperchromatic with finely granular chromatin. Macronucleoli are usually prominent. This study demonstrates that cytologic analysis of fine-needle aspirates can play a significant role in the evaluation and management of peripheral lung tumors.
Asunto(s)
Tumor Carcinoide/patología , Neoplasias Pulmonares/patología , Adenocarcinoma/patología , Anciano , Biopsia con Aguja/métodos , Carcinoma de Células Pequeñas/patología , Diagnóstico Diferencial , Femenino , Humanos , Masculino , Persona de Mediana EdadAsunto(s)
Carcinoma in Situ/diagnóstico , Displasia del Cuello del Útero/diagnóstico , Neoplasias del Cuello Uterino/diagnóstico , Carcinoma in Situ/patología , Colposcopía , Femenino , Humanos , Yoduros , Tamizaje Masivo , Displasia del Cuello del Útero/patología , Neoplasias del Cuello Uterino/patología , Frotis VaginalRESUMEN
The reported case consists in a 45-year-old patient with a mitral disease, who presented a bacterial endocarditis after a dental extraction, in the course of which he developed a mycotic aneurysm of the right superficial femoral artery; surgical treatment of this lesion was successful. One year later he was reoperated in relation with the progression of his previous mitral disease, and a Bjork-Shilley prosthesis was implanted. At the present time, 2 years and a half after the last operation the patient is free of symptoms. The clinical data, exploratory studies, the results which were obtained, and the surgical procedure applied are discussed. The rarity of this complication, the localization of the mycotic aneurysm, the surgical valve heart replacement, the pathologic findings of the resected valve and the satisfactory evolution of the patient are also pointed out.