RESUMEN
The dissection of urinary bladder is a crucial phase of the laparoscopic operation of hysterectomy. The latter dissection may become even more difficult in the context of history of operative interventions as cesarean section or cervical conization, presence of cervical or isthmic myomatous nodes, as well as upon a bad operative technique. The obeying of some basic principles (knowledge of pelvic anatomy and topographic interrelations; considering of present risk factors; intraoperative screening for complications and their early treatment) results in the optimal performance of laparoscopic hysterectomy.
Asunto(s)
Histerectomía/métodos , Laparoscopía/métodos , Vejiga Urinaria/cirugía , Útero/cirugía , Cuello del Útero/cirugía , Cesárea/efectos adversos , Cesárea/métodos , Conización/efectos adversos , Conización/métodos , Femenino , Humanos , Histerectomía/efectos adversos , Laparoscopía/efectos adversos , Mioma/cirugía , Neoplasias del Cuello Uterino/cirugíaRESUMEN
A method for separation and purification of plant hormones auxin and abscisic acid based on mixed mode reversed-phase anion-exchange solid phase extraction and two-dimensional HPLC was developed. Two-dimensional HPLC in "heart cutting" mode was very efficient in the purification of these two hormones. Its purification power is high enough to allow reliable on-line quantification of both hormones even with non-selective detectors.
Asunto(s)
Ácido Abscísico/aislamiento & purificación , Cromatografía Líquida de Alta Presión/métodos , Ácidos Indolacéticos/aislamiento & purificación , Reguladores del Crecimiento de las Plantas/aislamiento & purificación , Ácido Abscísico/análisis , Ácidos Indolacéticos/análisis , Reguladores del Crecimiento de las Plantas/análisis , Reproducibilidad de los ResultadosRESUMEN
Pain is a disagreeable subjective sensation. It is an emotional experience, related to actual or potential damage of the tissues and is a phenomenon, which is perceived unconsciously. As a frequently encountered suffering, it has definite social and economic significance. According to the pathogenesis, the pain also could be somatogenic, neurogenic and psychogenic. It has different site of occurrence: somatic (internal or external) and visceral. It evolves acutely or chronically. Clinically it is expressed as headache--primary (migraine or of straining type) and secondary (symptom of a definite illness). The cause of the pain could be inflammation (of the teeth, back pain, distortion, etc.), elevated temperature, etc. The convulsive pains could be related to menstruation. The modern treatment of the pain is carried out with nonsteroid antiinflammatory agents, which possess marked to different degree analgetic, temperature lowering and antiinflammatory action (aspirin, paracetamol, ibuprofen, etc.). In pain and particularly in headache the preparation Tomapyrin tabl. (Boehringer Ingelheim) is effective, which contains 250 mg acetylsalicylic acid, 200 mg paracetamol and 50 mg coffein. The alkaloid coffein stimulates the CNS, strengthens the striated musculature and displays marked vasoactive effect. The redistribution of the blood mass explains its effect in migraine. For exerting influence upon the pain relaxation programs have been created, physical therapy and training for mastering the stress are carried out.
Asunto(s)
Cefalea/tratamiento farmacológico , Dolor/tratamiento farmacológico , Enfermedad Aguda , Analgésicos/uso terapéutico , Enfermedad Crónica , Quimioterapia Combinada , Cefalea/clasificación , Cefalea/diagnóstico , Cefalea/etiología , Humanos , Dolor/clasificación , Dolor/diagnóstico , Dolor/etiologíaRESUMEN
Immunosorbents for the plant hormones cytokinins prepared by random antibody immobilization (to Affi-Gel 10) and by oriented approach via oxidized carbohydrate moieties on the Fc region (to Affi-Gel Hz or hydrazide derivative of Perloza MT 200) have been compared. Both approaches yielded immunosorbents with high dynamic capacity (ca. 5-10 nmol ml gel-1). Oriented antibody immobilization did not exhibit crucial effects in the case of low-molecular-mass cytokinins. Antibodies immobilized via a spacer to Affi-Gel 10 have probably enough conformational freedom to enable good accessibility to cytokinins. The sorbents were used in analysis of endogenous cytokinins in maize seeds. In phosphatase treated samples trans-zeatin and its riboside were predominant.
Asunto(s)
Anticuerpos/química , Citocininas/química , Celulosa , Cromatografía de Afinidad , Cromatografía Líquida de Alta Presión , Inmunoquímica , Inmunoglobulina G/química , Conformación Molecular , Oxidación-Reducción , Semillas/químicaRESUMEN
The disposition of ciprofloxacin and its pharmacologically active metabolites (sulfociprofloxacin, oxociprofloxacin, and desethylenciprofloxacin) in plasma, lung and bronchial tissues was studied in 24 patients undergoing a partial or total resection of the lung. The patients were divided into four groups, a control group and groups in which ciprofloxacin (200 mg) was given i.v. 1, 2 and 3 h before surgery. The concentrations of ciprofloxacin and its metabolites were determined by high performance liquid chromatography (HPLC). Ciprofloxacin concentrations in lung tissue were four times and in bronchial tissue twice those in plasma (p less than 0.01). The individual tissue concentrations of ciprofloxacin correlated with the individual plasma concentrations (r = 0.95 for lung; r = 0.94 for bronchi; p less than 0.001). Metabolite concentrations in both tissues and plasma were 10- to 100-fold lower than the concentrations of ciprofloxacin. These data suggest that the concentrations of the parent compound are essential for the therapeutic efficacy of ciprofloxacin.