RESUMEN
PURPOSE: Circulating cell-free DNA (cfDNA) is a promising biomarker for predicting treatment response and disease outcomes in Breast Cancer (BC) patients undergoing neoadjuvant chemotherapy (NAC). To determine if cfDNA originates from tumors, matching tumor and cfDNA gene mutations are necessary, often requiring tumor DNA sequencing. We assessed plasma cfDNA integrity by measuring concentrations and ratios of larger-to-smaller Alu DNA fractions as a potential biomarker, eliminating the need for prior tumor sequencing. METHODS: We included patients with localized and/or locally advanced BC receiving standard NAC alone or in combination with immunotherapy and/or anti-HER2 targeted therapy. Blood samples were collected before treatment, every 2 weeks during treatment, and before surgery. RESULTS: Of the 38 evaluated patients, only 28 completed the protocol and underwent surgery after NAC. Seven patients (25%) achieved a pathologic complete response (pCR). We found that cfDNA integrity (cfDNAI) levels at 15 days after starting NAC were significantly higher in patients who achieved pCR (p = 0.045) and correlated significantly with Disease-Free Survival (DFS) in univariate analysis (p = 0.0371). CONCLUSIONS: Evaluation of cfDNAI 2 weeks after NAC initiation appears to be an early biomarker for tumor pCR and DFS. Measuring Alu fragments of different lengths may replace techniques requiring prior tumor sequencing to measure ctDNA, reducing costs and complexity of cfDNA serial measurements in BC patients undergoing NAC.
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Protocolos de Quimioterapia Combinada Antineoplásica , Biomarcadores de Tumor , Neoplasias de la Mama , Ácidos Nucleicos Libres de Células , Terapia Neoadyuvante , Humanos , Femenino , Neoplasias de la Mama/tratamiento farmacológico , Neoplasias de la Mama/genética , Neoplasias de la Mama/sangre , Neoplasias de la Mama/patología , Terapia Neoadyuvante/métodos , Biomarcadores de Tumor/sangre , Persona de Mediana Edad , Proyectos Piloto , Adulto , Anciano , Ácidos Nucleicos Libres de Células/sangre , Ácidos Nucleicos Libres de Células/genética , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , ADN Tumoral Circulante/sangre , ADN Tumoral Circulante/genética , Resultado del Tratamiento , Pronóstico , Quimioterapia Adyuvante/métodosRESUMEN
The decision to implement a biogas production project involves the evaluation of multiple variables, such as the problem to be solved, the biodigester, business model, investment, and final products. An integrative literature review was conducted, in which 58 papers were obtained and relevant criteria for decision-making in biogas production projects from organic waste were identified. Three stages were considered in the analysis of the biogas production cycle: initial, plant, and final, as well as the economic, environmental, and social aspects that influence the decision. In general, the publications are dispersed over 30 different journals. The methodology used in most studies is empirical, quantitative, and descriptive, with data collected mainly from secondary sources. From the studies, 499 original criteria were identified, which were classified into one of four categories: economic, environmental, social, and technical, which cover a total of 39 sub-criteria. Economic and technical criteria were the most frequent in publications, while environmental and social criteria were less common and less prioritized. This suggests that there is a tendency to prioritize economic and technical dimensions over environmental and social dimensions in the analysis of the papers found. Finally, a preliminary decision-making model based on the findings is proposed.Implications: The integrative review of the literature on biogas and decision-making presented in this study holds significant implications for policy and practice in the field of sustainable energy production, organic waste management and decision making for public managers. By analyzing 159 papers and developing a comprehensive classification system, we have identified key sub-criteria for decision-making in various stages of the biogas production cycle. The predominance of technical and economic sub-criteria demonstrates the priorities of the current state of biogas projects and, at the same time, the need to promote balance between the technical, economic, social and environmental spheres in decision-making in order to achieve truly sustainable biogas projects. Policymakers can utilize these findings to promote the adoption of more holistic decision-making approaches that consider diverse factors, fostering the development of environmentally-friendly and socially equitable biogas ventures. This research provides valuable insights into aligning biogas production with broader sustainability goals and guides policymakers in formulating evidence-based strategies for the advancement of renewable energy technologies.
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Biocombustibles , Toma de Decisiones , Administración de Residuos/métodos , Administración de Residuos/economíaRESUMEN
Aniba canelilla (Kunth) Mez essential oil has many biological activities due to its main compound 1-nitro-2-phenylethane (1N2F), followed by methyleugenol, a carcinogenic agent. This study analyzed the influence of seasonality on yields, antioxidant capacity, and 1N2F content of A. canelilla leaf and twig essential oils. Essential oils (EOs) were extracted with hydrodistillation and analyzed with gas chromatography coupled to mass spectrometry and a flame ionization detector. Antioxidant capacity was measured using the free radical scavenging method (DPPH). Chemometric analyses were carried out to verify the influence of climatic factors on the production and composition of EOs. 1-Nitro-2-phenylethane was the major constituent in A. canelilla EOs throughout the seasonal period (68.0-89.9%); methyleugenol was not detected. Essential oil yields and the 1N2F average did not show a statistically significant difference between the dry and rainy seasons in leaves and twigs. Moderate and significant correlations between major compounds and climate factor were observed. The twig oils (36.0 ± 5.9%) a showed greater antioxidant capacity than the leaf oils (20.4 ± 5.0%). The PCA and HCA analyses showed no statistical differences between the oil samples from the dry and rainy seasons. The absence of methyleugenolin in all months of study, described for the first time, makes this specimen a reliable source of 1N2F.
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Lauraceae , Aceites Volátiles , Aceites Volátiles/química , Lauraceae/química , Estaciones del Año , Antioxidantes/farmacología , Cromatografía de Gases y Espectrometría de Masas , Hojas de la PlantaRESUMEN
PURPOSE: Describe sociodemographic and clinical characteristics of patients with hepatocellular carcinoma (HCC) and establish their history in the Brazilian public health system. METHODS: Retrospective observational study was conducted using the database from the Department of Informatics of the Unified Health System (DataSUS). Patients with at least one claim of HCC between July/2011 and June/2016 were included. A record linkage methodology was performed to obtain longitudinal data across different databases. Demographic and clinical data were evaluated, including the time elapsed between diagnosis of HCC risk-factors and the cancer development. Data was analyzed using descriptive statistics. RESULTS: A total of 28,822 HCC cases were identified between July/2011 and June/2016. Mean age was 59.7 years (SD = 14.7), and most patients were men (55.9%). The highest relative number of HCC cases was detected in the south of Brazil (> 20 cases/100,000 inhabitants). About 86.5% of the patients had diagnosis of HCC without previous liver diseases. Only 8% had diagnosis of chronic viral hepatitis and 3.5% cirrhosis. About 76% were diagnosed at an advanced stage, and only 11% of the patients had early stage HCC. Approximately 58% of patients with previous underlying liver diseases were diagnosed at early stages, compared with only 24% of patients without prior record of underlying diseases. CONCLUSION: The diagnosis of HCC in the Brazilian public health is usually made in patients with no previous diagnosis of liver disease and in advanced stages, when no curative treatment is available and survival rates are low. Public health policies are key for the screening and monitoring liver disease and, consequently, HCC.
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Carcinoma Hepatocelular/epidemiología , Neoplasias Hepáticas/epidemiología , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Brasil/epidemiología , Carcinoma Hepatocelular/patología , Niño , Preescolar , Bases de Datos Factuales , Femenino , Humanos , Lactante , Hepatopatías/diagnóstico , Hepatopatías/epidemiología , Neoplasias Hepáticas/patología , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Factores de Riesgo , Adulto JovenRESUMEN
The Eugenia and Syzygium genera include approximately 1000 and 1800 species, respectively, and both belong to the Myrtaceae. Their species present economic and medicinal importance and pharmacological properties. Due to their chemical diversity and biological activity, we are reporting the essential oils of 48 species of these two genera, which grow in South America and found mainly in Brazil. Chemically, a total of 127 oil samples have been described and displayed a higher intraspecific and interspecific diversity for both Eugenia spp. and Syzygium spp., according to the site of collection or seasonality. The main volatile compounds were sesquiterpene hydrocarbons and oxygenated sesquiterpenes, mainly with caryophyllane and germacrane skeletons and monoterpenes of mostly the pinane type. The oils presented many biological activities, especially antimicrobial (antifungal and antibacterial), anticholinesterase, anticancer (breast, gastric, melanoma, prostate), antiprotozoal (Leishmania spp.), antioxidant, acaricidal, antinociceptive and anti-inflammatory. These studies can contribute to the rational and economic exploration of Eugenia and Syzygium species once they have been identified as potent natural and alternative sources to the production of new herbal medicines.
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Antiinfecciosos/química , Antioxidantes/química , Eugenia/química , Aceites Volátiles/química , Aceites de Plantas/química , Syzygium/química , Antiinfecciosos/farmacología , Antioxidantes/farmacología , Aceites Volátiles/farmacología , Aceites de Plantas/farmacologíaRESUMEN
Stable isotopes have been widely used in the literature both to discuss current ocean circulation processes, as well as to reconstitute paleoceanographic parameters. The distribution of oxygen and deuterium stable isotopes in seawater (δ18Osw and δDsw) at the Western Tropical South Atlantic border was investigated to better understand the main fractionation processes of these isotopes and establish a regional salinity and δ18Osw relation to improve the paleoceanographic knowledge in the region. This study was conducted during a quasi-synoptic oceanographic cruise in which 98 discrete seawater samples were collected in the core of the main water masses for stable isotope analysis. A strong correlation between δ18Osw and δD was found, which made it possible to extrapolate the results for δ18Osw to δD. Although it was not possible to distinguish the water masses based only on their isotopic signatures, the water masses had a strong salinity and δ18Osw relation, and compared with previous studies, a seasonal pattern was observed. Paleosalinity differences of up to 0.2 psu between Summer and Winter are reported. Considering the limitations of the current techniques to seasonally separate the samples for the paleoceanographic studies, an intermediate Mixing Line for the Tropical South Atlantic (SSS = 1.942* δ18Osw + 34.56) was proposed to reduce the estimated errors associated with these seasonal variations.
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Isótopos de Oxígeno/química , Salinidad , Agua de Mar/química , Clima Tropical , Océano Atlántico , Brasil , Oceanografía , Estaciones del Año , TemperaturaRESUMEN
Abstract Formulations containing chitosan incorporated with methanolic fraction of Euphorbia umbellata (Pax) Bruyns, Euphorbiaceae, were studied aiming future applications of this new material as medicine. In order to investigate potential interactions between chitosan and the methanolic fraction (10, 50 and 100% in relation to the amount of chitosan) physicochemical characterization was performed by scanning electron microscopy, density, differential scanning calorimetry, thermogravimetry, X-ray diffraction, Fourier-transform infrared spectroscopy and colorimetry techniques. The phenolic compounds released from the chitosan membranes were evaluated using the Folin-Ciocalteau quantification method; antioxidant and antimicrobial activity were also studied. Increasing amounts of the methanolic fraction added to polymeric matrix produced different numbers of pores on the surface of the membranes, changes in the calorimetric, spectroscopic and crystalline properties as well as color changes, when compared to the inert membrane. These changes can be attributed to chemical interactions that occurred between the structure of the chitosan and the phenolic compounds present in the studied fraction. The matrix samples incorporated with 50 and 100% of the methanolic fraction presented different release profiles of phenolic compounds from the membranes (controlled manner) and promoted antioxidant and antimicrobial activity.
RESUMEN
Bragantina and Cingapura are the main black pepper (Piper nigrum L.) cultivars and the Pará state is the largest producer in Brazil with about 90% of national production, representing the third largest production in the world. The infection of Fusarium solani f. sp. piperis, the causal agent of Fusarium disease in black pepper, was monitored on the cultivars Bragantina (susceptible) and Cingapura (tolerant), during 45 days' post infection (dpi). Gas Chromatography-Mass spectrometry (GC-MS) analysis of the volatile concentrates of both cultivars showed that the Bragantina responded with the production of higher contents of α-bisabolol at 21 dpi and a decrease of elemol, mostly at 30 dpi; while Cingapura displayed an decrease of δ-elemene production, except at 15 dpi. The phenolic content determined by the Folin Ciocalteu method showed an increase in the leaves of plants inoculated at 7 dpi (Bragantina) and 7-15 dpi (Cingapura); in the roots, the infection caused a phenolic content decrease in Bragantina cultivar at 45 dpi and an increase in the Cingapura cultivar at 15, 30 and 45 dpi. High Performance Liquid Chromatography-Mass spectrometry (HPLC-MS) analysis of the root extracts showed a qualitative variation of alkamides during infection. The results indicated that there is a possible relationship between secondary metabolites and tolerance against phytopathogens.
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Resistencia a la Enfermedad , Metaboloma , Piper nigrum/metabolismo , Fusarium/patogenicidad , Sesquiterpenos Monocíclicos , Aceites Volátiles/metabolismo , Piper nigrum/genética , Piper nigrum/microbiología , Hojas de la Planta/metabolismo , Hojas de la Planta/microbiología , Sesquiterpenos/metabolismoRESUMEN
We present here a cross-sectional study analyzing the IgG1 and IgG2 immune responses to natural canine Leishmania (L.) infantum chagasi-infection and their relationships with delayed-type hypersensitivity (DTH) in 50 mongrel dogs with previous positive serodiagnoses (IFAT-IgG) (56% with subclinical status [= apparently healthy] and 44% clinically sick), living in endemic areas for visceral leishmaniasis in the Brazilian Amazon. IgG1 and IgG2 responses were measured using commercial polyclonal antibodies in ELISA, while DTH was elicited by intradermal skin test using cultured promastigotes L. (L.) i. chagasi-antigen. Data analyses used Chi-square and Pearson's r coefficient (95% confidence interval). Regarding DTH and the clinical statuses of dogs, it was noted that 100% of the animals showing positive DTH (n = 8) were from the subclinical group, while 100% showing negative DTH were from the clinically sick group; higher IgG2 than IgG1 responses were observed in both clinical groups. However, when this comparison was made between the subclinical and sick groups, higher IgG1 responses were noted in the dogs from the sick rather than the subclinical group, while no differences were noted between the IgG2 responses in the dogs from both clinical groups. Additionally, we found lower IgG1 responses in dogs from the subclinical group showing positive DTH than in the dogs from the subclinical or sick groups with negative DTH; no differences were found between the IgG2 responses of these two clinical groups. These findings suggest that the IgG1, but not the IgG2, response is associated with susceptibility to canine visceral leishmaniasis (CVL).
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Enfermedades de los Perros/inmunología , Inmunoglobulina G/sangre , Leishmaniasis Visceral/veterinaria , Distribución por Edad , Animales , Brasil/epidemiología , Intervalos de Confianza , Estudios Transversales , Susceptibilidad a Enfermedades/veterinaria , Enfermedades de los Perros/epidemiología , Enfermedades de los Perros/parasitología , Perros , Ensayo de Inmunoadsorción Enzimática/veterinaria , Femenino , Inmunoglobulina G/clasificación , Leishmaniasis Visceral/epidemiología , Leishmaniasis Visceral/inmunología , Masculino , Distribución por Sexo , Estadística como AsuntoRESUMEN
Acute kidney injury (AKI) from leptospirosis is frequently nonoliguric with hypo- or normokalemia. Higher serum potassium levels are observed in non-survivor patients and may have been caused by more severe AKI, metabolic disarrangement, or rhabdomyolysis. An association between the creatine phosphokinase (CPK) level and maximum serum creatinine level has been observed in these patients, which suggests that rhabdomyolysis contributes to severe AKI and hyperkalemia. LipL32 and Lp25 are conserved proteins in pathogenic strains of Leptospira spp., but these proteins have no known function. This study evaluated the effect of these proteins on renal function in guinea pigs. Lp25 is an outer membrane protein that appears responsible for the development of oliguric AKI associated with hyperkalemia induced by rhabdomyolysis (e.g., elevated CPK, uric acid and serum phosphate). This study is the first characterization of a leptospiral outer membrane protein that is associated with severe manifestations of leptospirosis. Therapeutic methods to attenuate this protein and inhibit rhabdomyolysis-induced AKI could protect animals and patients from severe forms of this disease and decrease mortality.
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Lesión Renal Aguda/patología , Proteínas de la Membrana Bacteriana Externa/metabolismo , Leptospirosis/complicaciones , Lipoproteínas/metabolismo , Rabdomiólisis/patología , Lesión Renal Aguda/microbiología , Animales , Creatina Quinasa/sangre , Creatinina/sangre , Modelos Animales de Enfermedad , Cobayas , Leptospira , Músculos/patología , Potasio/sangre , Rabdomiólisis/microbiologíaRESUMEN
This study aimed to evaluate the total extract of Taenia crassiceps metacestodes (TC) and its antigenic fractions obtained by Triton X-114 fractionation techniques, such as detergent (DC) and aqueous (AC), in the immunodiagnosis of human neurocysticercosis (NCC). Cerebrospinal fluid samples were divided into two groups: Group 1 (n=40), which was further divided into active (n=20) and inactive (n=20) NCC, and Group 2 (control group), which comprised 39 CSF samples from patients who had another neurological disorder, were suffering from other infectious diseases of the brain or had other parasitic infections. The total extracts and antigenic fractions were tested by enzyme-linked immunosorbent assay (ELISA) to detect human IgG anti-Taenia solium. T. crassiceps fractions (DC and AC) showed the same value of sensitivity (Se), 100%, for active and inactive NCC and a specificity (Sp) of 97.4%. The DS fraction obtained from T. solium showed 100% Se for active NCC, 95% Se for inactive NCC and a 92.3% Sp. The AS fraction obtained from T. solium showed 100% Se for both active and inactive NCC and a 94.9% Sp. There was a positive correlation between the total saline extract of T. crassiceps (TC) and T. solium (TS) and their fractions (DC, AC, DS and AS). Positive predictive value, negative predictive value, diagnostic efficiency and Youden index were calculated. In conclusion, these results demonstrated that detergent and aqueous fractions obtained from T. crassiceps metacestodes are important sources of specific antigens and are efficient for immunodiagnosis of active and inactive NCC.
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Anticuerpos Antihelmínticos/líquido cefalorraquídeo , Antígenos Helmínticos/inmunología , Antígenos Helmínticos/aislamiento & purificación , Ensayo de Inmunoadsorción Enzimática , Neurocisticercosis/diagnóstico , Neurocisticercosis/inmunología , Taenia/inmunología , Animales , Antígenos Helmínticos/química , Fraccionamiento Químico/métodos , Femenino , Humanos , Interacciones Hidrofóbicas e Hidrofílicas , Inmunoglobulina G/líquido cefalorraquídeo , Larva/química , Larva/inmunología , Masculino , Ratones , Neurocisticercosis/líquido cefalorraquídeo , Octoxinol , Polietilenglicoles , Valor Predictivo de las Pruebas , Sensibilidad y Especificidad , Cloruro de Sodio , Taenia/fisiologíaRESUMEN
Sickle cell disease (SCD) is a monogenic disease that affects millions of people worldwide. This study analyzed the effectiveness of bleaching and tooth sensitivity after in-office bleaching in patients with SCD. Forty volunteers were randomly assigned to four groups of 10 patients each (five with the SCD and five healthy controls) and treated using in-office bleaching with 35% hydrogen peroxide and different light activation protocols. No statistically significant difference was observed with relation to presence of tooth sensitivity, with or without use of a source of light for peroxide activation, and all bleaching therapies were effective, regardless of the technique employed and the presence/absence of sickle cell disease. The data showed that in-office dental bleaching is a viable alternative for improvement of oral esthetics for patients with SCD.
A doença falciforme (DF) é a doença monogênica e afeta milhões de pessoas em todo o mundo. Este estudo analisou a eficácia do clareamento e a presença de sensibilidade dentinária após clareamento dental em portadores de DF. Quarenta voluntários distribuídos aleatoriamente em quatro grupos com diferentes fontes de luz foram submetidos à técnica de clareamento em consultório, utilizando peróxido de hidrogênio a 35%. Não foram encontradas diferenças estatisticamente significativas em relação á presença de sensibilidade, com ou sem a utilização de uma fonte de luz para acelerar a ativação do peróxido e todos os clareamentos foram efetivos, independentemente da técnica utilizada e da presença/ausência de DF. Os dados mostraram que o clareamento dental em consultório, utilizando peróxido de hidrogênio a 35% é uma alternativa viável para melhoria da estética em pacientes com DF.
Asunto(s)
Anemia de Células Falciformes , Sensibilidad de la Dentina , Peróxido de Hidrógeno , Blanqueamiento de Dientes , Adolescente , Adulto , Anemia de Células Falciformes/complicaciones , Sensibilidad de la Dentina/etiología , Femenino , Humanos , Peróxido de Hidrógeno/efectos adversos , Masculino , Persona de Mediana Edad , Adulto JovenRESUMEN
Los hongos endofíticos son hongos que colonizan los tejidos internos de las plantas; varios compuestos biológicamente activos se han aislado a partir de estos hongos. Existen pocos estudios de compuestos aislados de hongos endófitos de plantas amazónicas. Por lo tanto, este estudio tuvo como objetivo el aislamiento y la identificación estructural de ergosterol (1), peróxido de ergosterol (2), mevalonolactona (3), citocalasina B (4) y citocalasina H (5) a partir de Aspergillus spp. EJC 04, un hongo endofítico de Bauhinia guianensis. La citocalasina B (4) y el derivado diacetato de citocalasina B (4a) mostraron una alta letalidad en el ensayo de Artemia salina. Esta es la primera aparición de citocalasinas en hongos endófitos amazónica de B. guianensis
Endophytic fungi are fungi that colonize internal tissues of plants; several biologically active compounds have been isolated from these fungi. There are few studies of compounds isolated from endophytic fungi of Amazon plants. Thus, this study aimed the isolation and structural identification of ergosterol (1), ergosterol peroxide (2), mevalonolactone (3), cytochalasin B (4) and cytochalasin H (5) from Aspergillus sp. EJC 04, an endophytic fungus from Bauhinia guianensis. The cytochalasin B (4) and the diacetate derivative of cytochalasin B (4a) showed high lethality in the brine shrimp assay. This is the first occurrence of cytochalasins in Amazonian endophytic fungi from B. guianensis
Asunto(s)
Artemia/efectos de los fármacos , Aspergillus/inmunología , Citocalasina B/aislamiento & purificación , Citocalasina B/análisis , Citocalasinas/aislamiento & purificación , Bauhinia/microbiología , Ergosterol/aislamiento & purificación , Endófitos/patogenicidadRESUMEN
Endophytic fungi are fungi that colonize internal tissues of plants; several biologically active compounds have been isolated from these fungi. There are few studies of compounds isolated from endophytic fungi of Amazon plants. Thus, this study aimed the isolation and structural identification of ergosterol (1), ergosterol peroxide (2), mevalonolactone (3), cytochalasin B (4) and cytochalasin H (5) from Aspergillus sp. EJC 04, an endophytic fungus from Bauhinia guianensis. The cytochalasin B (4) and the diacetate derivative of cytochalasin B (4a) showed high lethality in the brine shrimp assay. This is the first occurrence of cytochalasins in Amazonian endophytic fungi from B. guianensis.
Asunto(s)
Artemia/efectos de los fármacos , Aspergillus/química , Citocalasina B/toxicidad , Citocalasinas/toxicidad , Endófitos/química , Ergosterol/análogos & derivados , Fabaceae/microbiología , Ácido Mevalónico/análogos & derivados , Acetilación , Animales , Argentina , Aspergillus/aislamiento & purificación , Citocalasina B/química , Citocalasina B/aislamiento & purificación , Citocalasinas/química , Citocalasinas/aislamiento & purificación , Endófitos/aislamiento & purificación , Ergosterol/química , Ergosterol/aislamiento & purificación , Ergosterol/toxicidad , Dosificación Letal Mediana , Ácido Mevalónico/química , Ácido Mevalónico/aislamiento & purificación , Ácido Mevalónico/toxicidad , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Espectrometría de Masa por Ionización de Electrospray , Relación Estructura-ActividadRESUMEN
One of the problems frequently faced in laboratory facilities is the possibility of the natural parasitic infection of lab animals, which can interfere with biomedical research results. The present study aimed to evaluate cross-reactivity among serum samples from Wistar rats (Rattus norvegicus) naturally infected with Syphacia muris and experimentally infected with Strongyloides venezuelensis. Forty rats were divided into four groups of ten animals each. Parasite load was evaluated by quantifying the adult worms from both helminthes species recovered from the intestines and the S. venezuelensis eggs eliminated in feces. Serological cross-reactivity by parasite-specific IgG detection was tested via enzyme linked immunosorbent assay (ELISA), immunofluorescence antibody test (IFAT) and immunoblotting. The results demonstrated that the quantity of S. venezuelensis eliminated eggs and parthenogenetic females decreased significantly in cases of co-infection with S. muris. ELISA revealed 100% cross-reactivity of serum samples from both species against the opposing antigen. IgG cross-reactivity was confirmed by IFAT using tissue sections of S. venezuelensis larvae and adult S. muris. Immunoblotting showed that IgG antibodies from the sera of animals infected with S. muris recognized eight antigenic bands from S. venezuelensis saline extract and that IgG antibodies from the sera of animals infected with S. venezuelensis recognized seven bands from S. muris saline extract. These results demonstrate the serological cross-reactivity between S. muris and S. venezuelensis in infected rats.
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Antígenos Helmínticos/inmunología , Inmunoglobulina G/sangre , Oxiuriasis/inmunología , Oxyuroidea/inmunología , Strongyloides/inmunología , Estrongiloidiasis/inmunología , Animales , Coinfección , Reacciones Cruzadas , Ensayo de Inmunoadsorción Enzimática , Heces/parasitología , Femenino , Immunoblotting , Intestinos/parasitología , Larva , Oxiuriasis/complicaciones , Oxiuriasis/parasitología , Oxiuriasis/veterinaria , Carga de Parásitos , Ratas Wistar , Pruebas Serológicas , Estrongiloidiasis/complicaciones , Estrongiloidiasis/parasitologíaRESUMEN
Sickle cell disease (SCD) is a monogenic disease that affectsmillions of people worldwide. This study analyzed theeffectiveness of bleaching and tooth sensitivity after in-officebleaching in patients with SCD. Forty volunteers wererandomly assigned to four groups of 10 patients each (five withthe SCD and five healthy controls) and treated using in-officebleaching with 35% hydrogen peroxide and different lightactivation protocols. No statistically significant difference wasobserved with relation to presence of tooth sensitivity, with orwithout use of a source of light for peroxide activation, and allbleaching therapies were effective, regardless of the techniqueemployed and the presence/absence of sickle cell disease. Thedata showed that in-office dental bleaching is a viablealternative for improvement of oral esthetics for patients with SCD.
A doença falciforme (DF) é a doença monogênica e afetamilhões de pessoas em todo o mundo. Este estudo analisou aeficácia do clareamento e a presença de sensibilidadedentinária após clareamento dental em portadores de DF.Quarenta voluntários distribuídos aleatoriamente em quatrogrupos com diferentes fontes de luz foram submetidos à técnicade clareamento em consultório, utilizando peróxido dehidrogênio a 35%. Não foram encontradas diferenças estatisti -ca mente significativas em relação á presença de sensibilidade,com ou sem a utilização de uma fonte de luz para acelerar aativação do peróxido e todos os clareamentos foram efetivos,independentemente da técnica utilizada e da presença/ausênciade DF. Os dados mostraram que o clareamento dental em consultório, utilizando peróxido de hidrogênio a 35% é uma alternativa viável para melhoria da estética em pacientes com DF.
Asunto(s)
Humanos , Masculino , Adolescente , Adulto , Femenino , Adulto Joven , Anemia de Células Falciformes/complicaciones , Atención Dental para Enfermos Crónicos/métodos , Blanqueamiento de Dientes/efectos adversos , Sensibilidad de la Dentina/etiología , Brasil , Estética Dental , Peróxido de Hidrógeno/efectos adversos , Interpretación Estadística de DatosRESUMEN
Bauhinia guianensis is a typical plant in the Amazon region belonging to the family Leguminosea, used by local populations for the treatment of infectious and renal diseases. Previous work on the plant B. guianensis led to the isolation of substances with anti-inflammatory and analgesic activities. Thus, compounds isolated from B. guianensis with antimicrobial activities had not been identified. Given that there is a possibility of biological activity reported for a given plant being found in the endophytic fungi, we decided to isolate endophytic fungi from B. guianensis and test their antimicrobial activities. The alkaloids known as fumigaclavine C and pseurotin A were isolated by column chromatography and identified by 1D and 2D NMR techniques and mass spectrometry. The alkaloids are first reported as broad-spectrum antibacterial agents with good activity.