RESUMEN

Three major methods have been described for the isolation of fetal cells from maternal blood: fluorescence-activated cell sorting (FACS), immunomagnetic beads, and magnetic-activated cell sorting (MACS). To date, no study has directly compared fetal cell recovery using each of these methods. Here we describe our system using a "model' male fetal cell mixed into female peripheral blood mononuclear cells. Fetal cell yields and purities were assayed by a quantitative polymerase chain reaction (qPCR) using chromosomes Y- and 7-specific sequences. Fetal cell recovery was investigated by selection of CD71+ cells or depletion of CD45+ cells. Our data demonstrated variation in fetal cell recovery for all methods tested, although CD71+ selection by FACS gave the best and most consistent results.

Asunto(s)

Separación Celular/métodos , Feto/citología , Embarazo/sangre , Diagnóstico Prenatal/métodos , Antígenos CD/análisis , Antígenos de Diferenciación de Linfocitos B/análisis , Femenino , Feto/fisiología , Citometría de Flujo/métodos , Humanos , Separación Inmunomagnética , Técnicas In Vitro , Antígenos Comunes de Leucocito/análisis , Masculino , Reacción en Cadena de la Polimerasa , Receptores de Transferrina , Reproducibilidad de los Resultados