RESUMEN
The aqueous solutions of 3 acidic and 14 basic dyes used as reagents for detection of lipids without their destruction are tested on plates for highly effective TLCh with the silicagel layer fixed by the silicic acid sol. It is shown that 0.002% solution of basic fuchsin in 1% boric acid reveals neutral, phospho- and glycolipids with the sensitivity compared with such in detection of sulphuric acid. It is established that detection of lipids by this reagent with the use of TLCh does not change the composition of their fatty acids.
Asunto(s)
Cromatografía en Capa Delgada/instrumentación , Indicadores y Reactivos , Lípidos/análisisRESUMEN
Fragmentation of wall-eyed pollock and bovine rhodopsins by papain in the photoreceptor membrane was studied by sodium dodecyl sulfate electrophoresis. A scheme of step-wise rhodopsin proteolysis is presented. The molecular weights and localization of the carbohydrate and chromophore components of the fragments formed were determined. The data obtained suggest that the photoreceptor membranes of both rhodopsins contain three sites accessible to water environment and are indicative of topographical similarity of the rhodopsins.
Asunto(s)
Papaína , Pigmentos Retinianos , Rodopsina , Animales , Carbohidratos/análisis , Bovinos , Peces , Peso Molecular , Fragmentos de Péptidos/análisis , Células Fotorreceptoras/análisis , Especificidad de la EspecieAsunto(s)
Papaína/metabolismo , Células Fotorreceptoras/metabolismo , Pigmentos Retinianos/metabolismo , Rodopsina/metabolismo , Aminoácidos/análisis , Animales , Bovinos , Cloromercuribenzoatos/farmacología , Peces , Yodoacetamida/farmacología , Papaína/antagonistas & inhibidores , Papaína/farmacología , Fragmentos de Péptidos , Células Fotorreceptoras/efectos de los fármacos , Dodecil Sulfato de Sodio , Ácido Tricloroacético/farmacologíaRESUMEN
Regeneration of rhodopsin of the sea perch (Sebastodes schlegeli) and walleye pollock (Theragra chalcogramma) was studied in different preparations of eye after the addition of 11-cis- and all trans-retinals and at the expense of endogenic sources. It was established that the regeneration of rhodopsin in the eye cup, homogenate of retina and rod outer segments (ROS) at the expense of endogenic sources was extremely low and it was slightly forced by the addition of the pigment epithelium to the last two preparations. The binding degree of 11-cis retinal of walleye pollock ROS decreased with the increase of temperature of the incubation mixture and the increase of duration of storage of ROS at the temperature--10 degrees. Partial removing of lipids by hexane decreased the percentage of regeneration of the ROS preparation; on the other hand, the peroxidation of lipids practically did not affect the regenerative ability of fresh-obtained preparations. After solubilization of opsin by digitonin and sodium cholate, 40- and 50%-decreases of binding of 11-cis retinal were observed respectively. The digestion of ROS by papain, which caused no changes in the spectral properties of dark preparations, completely eliminated the regenerative ability of ROS. Regeneration of ROS at the presence of all trans-retinal proceeded at a very low speed. The specificity of the process of regeneration of rhodopsin in fishes is discussed.