RESUMEN
At the end of 1988 an outbreak of trichinellosis involving 48 persons occurred in a small town in Central Italy, near the Appennine mountains. The epidemic was caused by the ingestion of sausages recently made with wild boar meat. The people affected were mostly relatives and friends of hunters. Trichinella larvae were found in boar meat sausages and in cats fed with raw leftovers and proved to be Trichinella sp. 3 at biochemical typing. This species of parasite is the most frequently isolated species of Trichinella in Italy and other Southern European countries. Clinically the epidemic has shown a typical, uncomplicated febrile course in most patients, but two patients showed signs of cardiac damage and one intense abdominal pain, suggesting an acute abdomen.
Asunto(s)
Brotes de Enfermedades , Triquinelosis/epidemiología , Adolescente , Adulto , Animales , Anticuerpos Antihelmínticos/análisis , Femenino , Parasitología de Alimentos , Humanos , Italia/epidemiología , Masculino , Productos de la Carne , Prevalencia , Trichinella/inmunología , Trichinella/aislamiento & purificación , Triquinelosis/diagnóstico , Triquinelosis/etiologíaRESUMEN
The dissemination of a gentamicin resistant plasmid, originally found in strains of Klebsiella and termed pk181, into the microbial population of patients of the Orvieto Hospital was studied during 1982. Five hundred and seventy-four strains of Gram-negative bacilli were examined, transferable gentamicin resistance being revealed in five different bacterial species. The resistance was shown to be encoded by 81-megadalton plasmids in Escherichia coli and Enterobacter cloacae, and by 93-megadalton plasmids in Serratia marcescens and Pseudomonas spp. Restriction endonuclease digestion of plasmid DNA showed that the fragment patterns of the 81-megadalton plasmids from E. coli and enterobacter cloacae were identical to one another and to the pattern of plasmid pk181. The fragment patterns of the 93-megadalton plasmids from Serratia and Pseudomonas, on the contrary, differed substantially from those of the 81-megadalton plasmids.
Asunto(s)
Gentamicinas/farmacología , Bacterias Gramnegativas/genética , Factores R , Enzimas de Restricción del ADN , ADN Bacteriano/análisis , Farmacorresistencia Microbiana , Electroforesis en Gel de Agar , Enterobacter/efectos de los fármacos , Enterobacter/genética , Escherichia coli/efectos de los fármacos , Escherichia coli/genética , Bacterias Gramnegativas/efectos de los fármacos , Hospitales , Humanos , Pruebas de Sensibilidad Microbiana , Proteus mirabilis/efectos de los fármacos , Proteus mirabilis/genética , Pseudomonas/efectos de los fármacos , Pseudomonas/genética , Serratia marcescens/efectos de los fármacos , Serratia marcescens/genéticaRESUMEN
Two gentamicin resistant strains of Klebsiella were isolated at different times from patients with hospital acquired urinary tract infections. Both strains contained a conjugative 81-megadalton plasmid that encoded resistance to gentamicin, ampicillin, cephalothin and mercuric chloride. The plasmid conferred to Escherichia coli CSH26 the same pattern of resistance to eight aminoglycoside antibiotics and gave similar DNA fragments after restriction endonuclease digestion.