RESUMEN
A field study was initiated in 1988 to investigate whether spotted fever group rickettsiae occur in geographic areas in Egypt that are adjacent to an area in the southern Israeli Negev that has a defined focus of spotted fever disease. Ticks were collected from dogs, sheep, and camels at four study sites in the northern Sinai. Tick hemolymph was processed for rickettsial detection by staining with fluorescein isothiocyanate-conjugated antibody to Rickettsia rickettsii. Of the 442 hemolymphs examined, 15 contained immunofluorescent rickettsiae. Eight hemolymph test-positive (HT+) ticks were Rhipicephalus sanguineus removed from dogs; the other HT+ ticks comprised three Hyalomma species, H. anatolicum, H. impeltatum, and H. dromedarii. Both HT+ and HT- ticks were tested for rickettsial DNA using the polymerase chain reaction (PCR). Eight of 10 HT+ field-collected ticks were PCR positive (PCR+). All laboratory colony R. rickettsii-infected ticks were PCR+. No HT- ticks from field or laboratory isolates were PCR+.
Asunto(s)
Vectores Arácnidos/microbiología , Rickettsia rickettsii/aislamiento & purificación , Rickettsia/aislamiento & purificación , Garrapatas/microbiología , Animales , Secuencia de Bases , Camelus , ADN Bacteriano/análisis , Perros , Egipto , Electroforesis en Gel de Agar , Técnica del Anticuerpo Fluorescente , Cabras , Hemolinfa/microbiología , Datos de Secuencia Molecular , Sondas de Oligonucleótidos/química , Reacción en Cadena de la Polimerasa , Células VeroRESUMEN
13 children, 8 girls and 5 boys, aged 2-15 months (mean 8.5 months), with Haemophilus influenzae meningitis were treated with aztreonam. Aztreonam was administered intramuscularly in a dose of 50 mg/kg every 8 h. 10 of the infants were in coma and 3 were drowsy when first examined in hospital. All except 2 infants, who were in coma and who died within 24 h of starting therapy, recovered quickly. All patients became fully alert within 4-5 days and afebrile within 5 to 6 days.