RESUMEN

Several studies have described the use of non-invasive collection methods, mostly based on the detection of parasite DNA, for diagnosis. However, no Leishmania specimens have been isolated from saliva. Here, we report the first isolation of Leishmania braziliensis from the saliva of humans with cutaneous leishmaniasis but without lesions on their mucosa. The isolates were obtained from salivary fluid inoculated in hamsters and were tested by multilocus enzyme electrophoresis. Seven samples from 43 patients suspected of having the disease were identified for in vivo culture. These findings suggest that saliva is a clinical sample that allows the isolation of Leishmania sp.

Asunto(s)

Humanos , Masculino , Femenino , Adolescente , Adulto , Adulto Joven , Saliva/parasitología , Leishmania braziliensis/aislamiento & purificación , Reservorios de Enfermedades , Reacción en Cadena de la Polimerasa , Leishmaniasis Cutánea/diagnóstico , Leishmaniasis Cutánea/parasitología , Enfermedades Endémicas , Electroforesis , Persona de Mediana Edad