RESUMEN
BACKGROUND: Metabolic syndrome is a syndrome of a variety of metabolic disorders. Exercise is beneficial to the human body. However, the association of NR5A2 and exercise with metabolic syndrome remains unclear. METHODS: Download the GSE10540 and GSE12385 from GEO database. Bioinformatics analysis was used to screen the hub molecular of the metabolic syndrome. Forty 3-week-old C57BL/6J male mice were used in this study. The mean body weight was (17.5 ± 2.1) g. After 10 days of adaptive feeding, they were randomly divided into 4 groups according to the random number table method: Model + Exercise (n = 10), Model (n = 10), Model/NR5A2-OE (n = 10), Model/NR5A2-KO (n = 10). Western Blotting was performed to detect the expression of hub genes and signaling pathway. RESULTS: There were 349 DEGs in GSE10540 and 49 DEGs in GSE12385. 10 core genes were obtained. GO showed that differentially expressed genes were mainly enriched in vascular morphogenesis, contractile fiber fraction, chemotaxis, and MAPK cascade regulation. KEGG showed that MAPK signaling pathway was a significant section in the metabolic syndrome. PIK3R2, STRA8, FLT1, DMRT1, FGF22, NR5A2, and FLT were up-regulated and PRDM14, POU5F1, and KDR were down-regulated in metabolic syndrome after exercise. CONCLUSION: The expression of NR5A2 is down-regulated in metabolic syndrome, and exercise can increase the expression level of NR5A2. NR5A2 might be used as a potential target for exercise to improve metabolic syndrome.
Asunto(s)
Síndrome Metabólico , Ratones , Animales , Masculino , Humanos , Síndrome Metabólico/genética , Ratones Endogámicos C57BL , Biología Computacional , Perfilación de la Expresión Génica , Receptores Citoplasmáticos y NuclearesRESUMEN
OBJECTIVE: To explore the current condition of psychological problems of patients in cardiology clinic. METHODS: A total of 347 patients in the cardiology clinic of Prof. HU Da-yi were screened by self-rating depression scale (SDS), Self-rating anxiety scale (SAS), Hamilton rating scale for depression (HRSD) and Hamilton anxiety scale (HAMA). RESULTS: The rates of patients with somatic disease only or with psychological disorder only were 52.4% and 12.7%, the rate of somatic disease accompanied with psychological disorder was 27.7%. For the patients with psychological problems, there were 31 cases with depression (8.9%), 62 cases with anxiety (17.9%), and 28 cases (8.1%) suffering from mixed depression and anxiety the diagnosis of cardiovascular disease, functional disease or digestive disease was always used by doctors for the patients with psychological problems only, as they always did not accept the diagnosis of psychological disorder. CONCLUSIONS: It is very common that patients in cardiology clinic department suffered from psychological problems. The patients with psychological disorder only were easily misdiagnosed as somatic disease especially cardiovascular disease.