RESUMEN

A fast and simple approach for immobilization using copolymers as interlayers is reported. The synthesized copolymers form stable self-assembled layers on implant materials like, e.g., titanium in a simple coating/drying/washing sequence and have functional groups which can bind proteins from an aqueous solution. The copolymer films have been characterized via ellipsometry and contact angle measurements and were tested for biocompatibility. An immunoassay was used to determine the amount of BMP2 and demonstrated an approximately 10-fold increase as compared to previously used self-assembled monolayers. A BMP2-responsive cell line with luciferase detection was used to determine the biological activity of the bound signaling protein.

Asunto(s)

Proteína Morfogenética Ósea 2/metabolismo , Materiales Biocompatibles Revestidos/farmacología , Polímeros/farmacología , Prótesis e Implantes , Transducción de Señal/efectos de los fármacos , Titanio/farmacología , Animales , Proliferación Celular/efectos de los fármacos , Ensayo de Inmunoadsorción Enzimática , Humanos , Proteínas Inmovilizadas/metabolismo , Espectroscopía de Resonancia Magnética , Ratones , Polimerizacion/efectos de los fármacos , Unión Proteica/efectos de los fármacos