RESUMEN
In this work we have investigated the molecular basis of the neuronal damage induced by the prion peptide by searching for a surface receptor whose activation could be the first step of a cascade of events responsible for cell death. By using a human neuroblastoma cell line lacking all the neurotrophin receptors and derived clones expressing the full-length or truncated forms of the low affinity neurotrophin receptor (p75(NTR)), we have been able to demonstrate that the neuronal death induced by the prion protein fragment PrP-(106-126) is an active process mediated by a) the binding of the peptide to the extracellular region of p75(NTR), b) the signaling function of the intracytoplasmic region of the receptor, and c) the activation of caspase-8 and the production of oxidant species.
Asunto(s)
Neuronas/metabolismo , Fragmentos de Péptidos/metabolismo , Priones/metabolismo , Receptores de Factor de Crecimiento Nervioso/química , Receptores de Factor de Crecimiento Nervioso/metabolismo , Sitios de Unión , Northern Blotting , Western Blotting , Caspasa 8 , Caspasa 9 , Caspasas/metabolismo , Relación Dosis-Respuesta a Droga , Activación Enzimática , Inhibidores Enzimáticos/farmacología , Humanos , Microscopía Fluorescente , Neuroblastoma/metabolismo , Oxígeno/metabolismo , Unión Proteica , Receptor de Factor de Crecimiento Nervioso , Estaurosporina/farmacología , Factores de Tiempo , Células Tumorales CultivadasRESUMEN
Helicobacter pylori infection induces the appearance of inflammatory infiltrates, consisting mainly of neutrophils and monocytes, in the human gastric mucosa. A bacterial protein with neutrophil activating activity (HP-NAP) has been previously identified, but its role in infection and immune response is still largely unknown. Here, we show that vaccination of mice with HP-NAP induces protection against H. pylori challenge, and that the majority of infected patients produce antibodies specific for HP-NAP, suggesting an important role of this factor in immunity. We also show that HP-NAP is chemotactic for human leukocytes and that it activates their NADPH oxidase to produce reactive oxygen intermediates, as demonstrated by the translocation of its cytosolic subunits to the plasma membrane, and by the lack of activity on chronic granulomatous disease leukocytes. This stimulating effect is strongly potentiated by tumor necrosis factor alpha and interferon gamma and is mediated by a rapid increase of the cytosolic calcium concentration. The activation of leukocytes induced by HP-NAP is completely inhibited by pertussis toxin, wortmannin, and PP1. On the basis of these results, we conclude that HP-NAP is a virulence factor important for the H. pylori pathogenic effects at the site of infection and a candidate antigen for vaccine development.
Asunto(s)
Antígenos Bacterianos/inmunología , Proteínas Bacterianas/inmunología , Infecciones por Helicobacter/prevención & control , Helicobacter pylori/patogenicidad , Neutrófilos/inmunología , Androstadienos/farmacología , Animales , Anticuerpos Antibacterianos/sangre , Señalización del Calcio , Quimiotaxis de Leucocito , Citosol/metabolismo , Helicobacter pylori/inmunología , Humanos , Integrinas/biosíntesis , Ratones , Monocitos/inmunología , NADPH Oxidasas/metabolismo , Toxina del Pertussis , Proteínas/farmacología , Especies Reactivas de Oxígeno , Vacunación , Factores de Virulencia de Bordetella/farmacología , WortmaninaRESUMEN
This study concerns the controversial problem of whether the TNF-alpha (TNF) induces a respiratory burst in human neutrophils in suspension. The results have shown that in these cells TNF induces a classical respiratory burst. In fact, the production of oxygen free radicals 1) is linked to the translocation of NADPH oxidase components from cytosol to the plasma membrane, 2) does not take place in neutrophils from a patient lacking the cytochrome b558, and 3) does not involve other sources such as mitochondrial respiratory chain or xanthine oxidase. Signal transduction studies have demonstrated that this respiratory burst 1) is not accompanied by calcium transients, stimulation of phosphoinositide turnover, and phospholipase D activity (moreover, this burst is associated with the stimulation of the activity of phospholipase A2, but not of sphingomyelinase); 2) is strictly dependent on activation of tyrosine kinases, which is functional to the translocation to the plasma membrane of the cytosolic NADPH oxidase component rac; and 3) is dependent on the integrity of the cytoskeleton because it is completely suppressed by cytochalasin B. The integrity of the cytoskeleton is required for a full translocation of all the NADPH oxidase components and for an optimal activation of tyrosine kinases, but not for phospholipase A2 activation. Taken together, these findings demonstrate that TNF activates the NADPH oxidase through stimulation of tyrosine kinases, whose function is cytoskeleton-dependent, and raise the problem of whether the activation of this respiratory burst involves signals arising from TNF-activated beta2 integrins.
Asunto(s)
Citoesqueleto/inmunología , Lípidos de la Membrana/sangre , Lípidos de la Membrana/inmunología , Neutrófilos/inmunología , Proteínas Tirosina Quinasas/farmacología , Estallido Respiratorio/inmunología , Transducción de Señal/inmunología , Factor de Necrosis Tumoral alfa/farmacología , Separación Celular , Citocalasina B/farmacología , Citoesqueleto/efectos de los fármacos , Activación Enzimática/inmunología , Genisteína , Humanos , Isoflavonas/farmacología , Lípidos de la Membrana/metabolismo , NADPH Oxidasas/antagonistas & inhibidores , NADPH Oxidasas/biosíntesis , Fosfolipasas A/antagonistas & inhibidores , Fosfolipasas A2 , Fosforilación , Proteínas Tirosina Quinasas/antagonistas & inhibidoresRESUMEN
Human neutrophils respond with an increased phagocytosis when exposed to TNF. Two types of TNF receptors have been identified, namely 55 kDa (TR55) and 75 kDa (TR75). We addressed the problem of the role of these receptors in the priming effect of TNF. By using monoclonal antibodies (MoAbs) directed either against TR55 or TR75, we have shown that 1) only TR55 is the signaling receptor for the potentiation of Fc-mediated phagocytosis and upregulation of beta 2-integrin CD11b/CD18; 2) TR75 may control the function of TR55 by regulating the binding of TNF to the signaling receptor.
Asunto(s)
Antígenos CD/fisiología , Fragmentos Fc de Inmunoglobulinas/fisiología , Neutrófilos/inmunología , Fagocitosis/fisiología , Receptores del Factor de Necrosis Tumoral/fisiología , Anticuerpos Monoclonales/inmunología , Antígenos CD/inmunología , Humanos , Técnicas In Vitro , Receptores del Factor de Necrosis Tumoral/inmunología , Receptores Tipo I de Factores de Necrosis Tumoral , Receptores Tipo II del Factor de Necrosis TumoralRESUMEN
The stimulation of phosphoinositide hydrolysis by a number of agonists (phosphoinositide response) is a ubiquitous transmembrane signalling process for the regulation of several cell functions. Two mechanisms of activation have been identified that involve different phospholipases C: one regulated by G-proteins and another regulated by receptors having an intrinsic tyrosine kinase domain or that stimulate intracellular tyrosine kinase activity. This last mechanism is activated in several immunological cells, including lymphocytes, mastocytes, NK cells and monocytes, in response to agonists that bind antigen receptors, and receptors for IgE and IgG. In the present study, we have investigated the role of tyrosine phosphorylation in the stimulation of phosphoinositide hydrolysis mediated by Fc gamma Rs in human neutrophils. The results demonstrated that: 1) the activation of Fc gamma Rs with insoluble immune complexes (IIC) induced a tyrosine phosphorylation of several proteins that was dose-dependently inhibited by the tyrosine kinase inhibitor, genistein; 2) the activation of Fc gamma Rs caused a stimulation of phosphoinositide hydrolysis measured as [3H]inositol phosphates formation; 3) genistein depressed the activation of phosphoinositide hydrolysis; 4) among the several proteins that became tyrosine phosphorylated upon Fc gamma Rs activation by IIC, one 145 kDa protein was identified as PLC-gamma 2, using a specific antiserum. The phosphorylation of PLC-gamma 2 was completely inhibited by genistein. These results demonstrate that the phosphoinositide response to activation of Fc gamma Rs involves the tyrosine phosphorylation of PLC-gamma 2.
Asunto(s)
Neutrófilos/fisiología , Fosfatidilinositoles/metabolismo , Receptores Fc/metabolismo , Fosfolipasas de Tipo C/metabolismo , Tirosina/análogos & derivados , Genisteína , Humanos , Isoenzimas , Isoflavonas/farmacología , Fosfoproteínas/metabolismo , Fosfotirosina , Agregación de Receptores , Transducción de Señal , Factores de Tiempo , Tirosina/metabolismoRESUMEN
It has been recently suggested that protein tyrosine phosphorylation is involved in Fc gamma Rs-mediated signalling. In this paper we have investigated if in human neutrophils a tyrosine phosphorylation of Fc gamma RII takes places after the binding with immunocomplexes and if this phosphorylation plays a role in phagocytic signal. The immunoprecipitation with mAb anti-Fc gamma RII of lysates of neutrophils challenged in suspension with insoluble immunocomplexes (IIC) or sheep erythrocytes opsonized with IgG (E-IgG), followed by immunoblotting with anti-phosphotyrosine antibody, demonstrated that Fc gamma RII was tyrosine phosphorylated. When neutrophils were pretreated with different doses of tyrosine kinase inhibitors, genistein or erbstatin, the phosphorylation of Fc gamma RII induced by IIC or E-IgG was dose dependently inhibited. In these conditions both genistein and erbstatin failed to inhibit the phagocytosis of E-IgG. These results demonstrated that in human neutrophils in suspension the binding to Fc of IgG induces a tyrosine phosphorylation of Fc gamma RII but this phosphorylation is not an essential signal for phagocytosis of IgG-opsonized particles.
Asunto(s)
Complejo Antígeno-Anticuerpo/metabolismo , Neutrófilos/inmunología , Fagocitosis , Receptores de IgG/metabolismo , Genisteína , Humanos , Hidroquinonas/farmacología , Técnicas In Vitro , Isoflavonas/farmacología , Neutrófilos/metabolismo , Fagocitosis/efectos de los fármacos , Fosforilación , Fosfotirosina , Tirosina/análogos & derivados , Tirosina/metabolismoRESUMEN
The correlation between the increase of [Ca2+]i and the activation of hydrolysis of phosphoinositide-4,5-bisphosphate and formation of inositol(1,4,5)trisphosphate in neutrophils treated with Fc receptor-binding agonists is still under discussion. In this communication evidence is presented supporting the conclusion that, as it is widely accepted for the activation of other receptors, also upon the activation of Fc receptors the stimulation of the production of inositol(1,4,5) trisphosphate is involved in the increase in [Ca2+]i. In fact: i) treatment of neutrophils with immune complexes induced a very rapid phosphoinositide hydrolysis measured as [3H]inositol phosphates production from [3H]phosphoinositides and as inositol(1,4,5) trisphosphate formation measured with radioreceptor assay, ii) immune complexes caused a dose-dependent increase of [Ca2+]i; iii) the increase of [Ca2+]i correlated with the production of inositol(1,4,5) trisphosphate with respect to time course, dose dependence and pertussis toxin insensitivity.
Asunto(s)
Calcio/sangre , Inositol 1,4,5-Trifosfato/sangre , Neutrófilos/metabolismo , Receptores Fc/fisiología , Complejo Antígeno-Anticuerpo/farmacología , Citosol/metabolismo , Humanos , Técnicas In Vitro , Fosfatos de Inositol/sangre , Cinética , N-Formilmetionina Leucil-Fenilalanina/farmacología , Neutrófilos/efectos de los fármacos , Neutrófilos/inmunología , Toxina del Pertussis , Fosfatidilinositoles/sangre , Receptores Fc/efectos de los fármacos , Factores de Virulencia de Bordetella/farmacologíaRESUMEN
The results presented in this paper demonstrate that the potentiation of phagocytosis of erythrocyte (E) IgG by TNF-alpha or PMA is not due to an oxygen-dependent mechanism. In fact, the potentiation of phagocytosis occurs normally in human neutrophils 1) when the respiratory burst is inhibited by diphenyleneiodonium, 2) in conditions where the reactive oxygen metabolites produced by the activation of NADPH oxidase, that accompanies the phagocytosis, were removed by catalase or superoxide dismutase, 3) of a patient lacking NADPH oxidase activity due to a genetic defect of p67-phox, 4) treated with staurosporine which allowed PMA to potentiate the ingestion of E-IgG at concentrations which inhibited the activation of the respiratory burst. Evidence is also presented that staurosporine not only did not inhibit, but amplified the potentiation of phagocytosis by PMA and TNF-alpha. This last finding suggests that the activation of protein kinase C plays a modulatory rather than a positive role in the mechanism of potentiation of phagocytosis.
Asunto(s)
NADH NADPH Oxidorreductasas/sangre , Neutrófilos/fisiología , Fagocitosis/efectos de los fármacos , Proteína Quinasa C/sangre , Receptores Fc/metabolismo , Acetato de Tetradecanoilforbol/farmacología , Factor de Necrosis Tumoral alfa/farmacología , Alcaloides/farmacología , Relación Dosis-Respuesta a Droga , Humanos , Inmunoglobulina G/metabolismo , Técnicas In Vitro , Cinética , NADH NADPH Oxidorreductasas/antagonistas & inhibidores , NADPH Oxidasas , Neutrófilos/efectos de los fármacos , Neutrófilos/inmunología , Compuestos Onio/farmacología , Proteína Quinasa C/antagonistas & inhibidores , Receptores Fc/efectos de los fármacos , Estaurosporina , Superóxido Dismutasa/farmacología , Superóxidos/sangreRESUMEN
We have previously shown that in neutrophils classical transmembrane signaling consisting of increased [Ca2+]i and hydrolysis of phospholipids was not essential for phagocytosis mediated by more than one receptor (yeast-IgG, yeast-C3b/bi, yeast-Con A). This work deals with the role of this transmembrane signaling in phagocytosis of erythrocyte (E) IgG, which is mediated only by receptors for IgG (Fc gamma Rs). The ingestion of E-IgG was associated with an increase in [Ca2+]i and production of inositol phosphates, phosphatidic acid, diacylglycerol, and arachidonic acid, via activation of phospholipases C, D and A2. Related to the same number of particles ingested, the respiratory burst and the transmembrane signaling during phagocytosis of E-IgG were much smaller than during phagocytosis of yeast-IgG. In Ca(2+)-depleted neutrophils, where the increase in [Ca2+]i and hydrolysis of phospholipids were lacking, the phagocytosis of E-IgG was depressed by about 60%; the respiratory burst was also depressed due to the decrease of ingestion and of stimulation of NADPH oxidase by residual phagocytosis. Pertussis toxin (PT) did not inhibit the phagocytosis of E-IgG but depressed by about 40% the stimulation of lipidic transmembrane signaling and the respiratory burst in normal neutrophils. In Ca(2+)-depleted neutrophils the toxin was without effect on ingestion and respiratory burst. Staurosporine did not inhibit the ingestion of E-IgG in normal and Ca(2+)-depleted neutrophils but depressed by 30-40% the respiratory burst in normal and not in Ca(2+)-depleted neutrophils. Genistein, an inhibitor of tyrosine kinase, did not inhibit the ingestion of E-IgG but depressed by 30-40% the respiratory burst both in normal and Ca(2+)-depleted neutrophils. These results demonstrate the following findings in human neutrophils. (1) Contrary to the phagocytosis mediated by more than one receptor (yeast-IgG, yeast-Con A, yeast-C3b/bi), the transmembrane signaling involving increase in [Ca2+]i and hydrolysis of phospholipids plays a role in the phagocytosis and respiratory burst mediated by Fc gamma Rs alone. Thus, different signal transduction pathways can be involved in phagocytosis and associated respiratory burst depending on the receptor or combination of receptors activated. (2) Fc gamma Rs alone promote phagocytosis with two signaling pathways independent of and dependent on [Ca2+]i changes and phospholipid hydrolysis and insensitive to PT, staurosporine, and genistein. (3) The signaling pathways promoting phagocytosis triggered by Fc gamma Rs alone are in some way, or at some step, different from those that activate the respiratory burst.
Asunto(s)
Inmunoglobulina G/metabolismo , NADH NADPH Oxidorreductasas/sangre , Neutrófilos/fisiología , Fagocitosis , Receptores de IgG/fisiología , Transducción de Señal , Ácido Araquidónico/sangre , Calcio/sangre , Activación Enzimática , Eritrocitos/inmunología , Humanos , Técnicas In Vitro , Fosfatos de Inositol/sangre , Cinética , N-Formilmetionina Leucil-Fenilalanina/farmacología , NADPH Oxidasas , Neutrófilos/efectos de los fármacos , Neutrófilos/inmunología , Consumo de Oxígeno , Fagocitosis/efectos de los fármacos , Fosfolípidos/sangre , Acetato de Tetradecanoilforbol/farmacologíaRESUMEN
Stimulation of neutrophils with different agonists activates a latent multicomponent NADPH oxidase that reduces molecular oxygen to superoxide anion. Evidence has accumulated that phosphorylation of p47phox (the 47 kDa cytosolic phagocyte oxidase factor) and translocation of the two cytosolic components p47phox and p67phox are essential steps in the activation of NADPH oxidase in response to phorbol esters. We analysed the relationships between activation of the NADPH oxidase and phosphorylation and translocation of p47phox and p67phox in normal and Ca(2+)-depleted neutrophils stimulated by the receptor-mediated agonists formyl-methionyl-leucyl-phenylalanine and concanavalin A. The results produced the following conclusions: (1) Translocation of p47phox and p67phox is an essential mechanism for activation of the NADPH oxidase. (2) A continuous translocation of p47phox and p67phox is necessary to maintain the NADPH oxidase in an activated state. (3) Only a fraction of p47phox and p67phox translocated to the plasma membrane is functional for the activation of the oxidase. (4) Translocation is independent of protein kinase C, and is linked to transmembrane signalling involving Ca2+ transients and production of lipidic second messengers. However, under some conditions, such as in Ca(2+)-depleted neutrophils, translocation can also occur independently of signalling pathways involving production of second messengers from hydrolysis of phospholipids and Ca2+ transients. (5) Phosphorylation of p47phox and p67phox can be quantitatively dissociated from translocation, as staurosporine markedly inhibits phosphorylation but not translocation. (6) The activity of NADPH oxidase is not correlated with the amounts of the phosphorylated proteins present in the plasma membrane.
Asunto(s)
Calcio/metabolismo , Membrana Celular/metabolismo , NADH NADPH Oxidorreductasas/metabolismo , NADPH Deshidrogenasa/metabolismo , Neutrófilos/metabolismo , Fosfoproteínas/metabolismo , Alcaloides/farmacología , Transporte Biológico , Concanavalina A/farmacología , Activación Enzimática , Humanos , Cinética , N-Formilmetionina Leucil-Fenilalanina/farmacología , NADPH Oxidasas , Neutrófilos/efectos de los fármacos , Fosforilación , Transducción de Señal/fisiología , EstaurosporinaRESUMEN
The results presented in this paper demonstrate that in human neutrophils phagocytosis of C3b/bi and IgG-opsonized yeast particles is associated with activation of phospholipase D and that this reaction is the main source of diglycerides. The demonstration is based upon the following findings: 1) the challenge of neutrophils with these opsonized particles was followed by a rapid formation of [3H]alkyl-phosphatidic acid [( 3H]alkyl-PA) and [3H]alkyl-diglyceride [( 3H]alkyl-DG) in cells labeled with [3H]alkyl-lyso-phosphatidylcholine; 2) in the presence of ethanol [3H]alkyl-phosphatidylethanol was formed, and accumulation of [3H]alkyl-PA and [3H]alkyl-DG was depressed; 3) propranolol, by inhibiting the dephosphorylation of [3H]alkyl-PA, completely inhibited the accumulation of [3H]alkyl-DG and depressed by about 75% the formation of diglyceride mass. Evidence is also presented that phagocytosis of C3b/bi and IgG-opsonized yeast particles and associated respiratory burst can take place independently of diglyceride formation and of the activity of this second messenger on protein kinase C. In fact: a) propranolol while completely inhibited the formation of diglyceride mass did not modify either the phagocytosis or respiratory burst; b) these two processes were insensitive to staurosporine.
Asunto(s)
Diglicéridos/sangre , Neutrófilos/fisiología , Consumo de Oxígeno , Fagocitosis , Fosfolípidos/sangre , Alcaloides/farmacología , Complemento C3b/fisiología , Humanos , Inmunoglobulina G , Técnicas In Vitro , Cinética , N-Formilmetionina Leucil-Fenilalanina/farmacología , Neutrófilos/efectos de los fármacos , Consumo de Oxígeno/efectos de los fármacos , Fagocitosis/efectos de los fármacos , Fosfolipasa D/sangre , Propranolol/farmacología , Proteína Quinasa C/antagonistas & inhibidores , Saccharomyces cerevisiae , Estaurosporina , Acetato de Tetradecanoilforbol/farmacología , TritioRESUMEN
The phagocytosis of beta-glucan particles by human neutrophils and the associated activation of NADPH O2- forming oxidase were accompanied by an increased hydrolysis of phosphoinositides by phospholipase C, hydrolysis of phosphatidylcholine by phospholipase D, accumulation of diglyceride (DG) mass, and [Ca2+]i rise. The reaction of phospholipid hydrolysis played a minor role in the formation of DG, which was mainly formed by de novo synthesis from glucose. The activation of this pathway was shown by the stimulation of the incorporation of [U-14C]glucose into DG, which occurred very rapidly after the challenge of neutrophils with beta-glucan particles. This DG derived from glucose was found almost completely as 1-acyl-2-acyl-glycerol (DAG). On the basis of the finding that phosphatidic acid was the precursor of DAG, an increase in the incorporation of [U-14C]acetate into DAG did not occur, and the [14C]radioactivity was in the glycerol backbone, the synthesis of DAG from [U-14C]glucose occurred very likely via dihydroxyacetone phosphate and glycerol 3-phosphate, stepwise acylation to phosphatidic acid, and dephosphorylation by phosphatidate phosphatase.
Asunto(s)
Diglicéridos/biosíntesis , Glucanos/inmunología , Glucosa/metabolismo , Neutrófilos/metabolismo , Fagocitosis , Transducción de Señal , Calcio/metabolismo , Dihidroxiacetona Fosfato/metabolismo , Activación Enzimática , Glicerofosfatos/metabolismo , Humanos , Técnicas In Vitro , Cinética , NADH NADPH Oxidorreductasas/metabolismo , NADPH Oxidasas , Fosfatidilcolinas/metabolismo , Fosfatidilinositoles/metabolismo , Fosfolipasa D/metabolismo , Fosfolipasas de Tipo C/metabolismoRESUMEN
It is widely accepted that the activation of the NADPH oxidase of phagocytes is linked to the stimulation of protein kinase C by diacylglycerol formed by hydrolysis of phospholipids. The main source would be choline containing phospholipid via phospholipase D and phosphatidate phosphohydrolase. This paper presents a condition where the activation of the respiratory burst by FMLP correlates with the formation of phosphatidic acid, via phospholipase D, and not with that of diacylglycerol. In fact: 1) in neutrophils treated with propranolol, an inhibitor of phosphatidate phosphohydrolase, FMLP plus cytochalasin B induces a respiratory burst associated with a stimulation of phospholipase D, formation of phosphatidic acid and complete inhibition of that of diacylglycerol. 2) The respiratory burst by FMLP plus cytochalasin B lasts a few minutes and may be restimulated by propranolol which induces an accumulation of phosphatidic acid. 3) In neutrophils stimulated by FMLP in the absence of cytochalasin B propranolol causes an accumulation of phosphatidic acid and a marked enhancement of the respiratory burst without formation of diacylglycerol. 4) The inhibition of the formation of phosphatidic acid via phospholipase D by butanol inhibits the respiratory burst by FMLP.
Asunto(s)
Diglicéridos/fisiología , Glicéridos/fisiología , NADH NADPH Oxidorreductasas/metabolismo , Neutrófilos/fisiología , Ácidos Fosfatidicos/fisiología , Fosfolipasa D/fisiología , Fosfolipasas/fisiología , Butanoles/farmacología , Citocalasina B/farmacología , Activación Enzimática/efectos de los fármacos , Humanos , Técnicas In Vitro , N-Formilmetionina Leucil-Fenilalanina/farmacología , NADPH Oxidasas , Consumo de Oxígeno/efectos de los fármacos , Propranolol/farmacologíaRESUMEN
The role of messengers derived from hydrolysis of phosphoinositides and other phospholipids, of the basal level of [Ca2+]i and of the increase in [Ca2+]i in phagocytosis and respiratory burst was investigated, using normal neutrophils and neutrophils Ca2(+)-depleted by pretreatment with Quin2/AM and EGTA. 1) Phagocytosis and respiratory burst in control neutrophils challenged with yeast opsonized with IgG or C3b/bi were associated with a stimulation of the production of inositol phosphates, diacylglycerol, phosphatidic acid, arachidonic acid, and rise in [Ca2+]i. 2) In Ca2(+)-depleted neutrophils (basal [Ca2+]i 10 to 20 nM) the phagocytosis of yeast-IgG was similar to that in control neutrophils, the respiratory burst was slightly depressed (-30%), while the increase in [Ca2+]i and production of inositol phosphates, diacylglycerol, and phosphatidic and arachidonic acid did not occur. 3) In Ca2(+)-depleted neutrophils the phagocytosis of yeast-C3b/bi was slightly lower than that in control neutrophils, and the respiratory burst, related to the same number of particles ingested, was depressed by about 60%, whereas the increase in [Ca2+]i and production of inositol phosphates, diacylglycerol, phosphatidic acid, and arachidonic acid release did not occur. These findings demonstrate that transmembrane signaling pathways involving the hydrolysis of phosphoinositides by phospholipase C and D and of other phospholipids by phospholipase C and Az, and the rise in [Ca2+]i are not essential processes for triggering the ingestion of yeast particles opsonized with IgG and C3b/bi and the activation of the NADPH oxidase.
Asunto(s)
Calcio/metabolismo , NADH NADPH Oxidorreductasas/metabolismo , Neutrófilos/fisiología , Fagocitosis , Fosfolípidos/metabolismo , Ácido Araquidónico , Ácidos Araquidónicos/metabolismo , Complemento C3b/fisiología , Diglicéridos/metabolismo , Activación Enzimática , Humanos , Inmunoglobulina G/fisiología , Técnicas In Vitro , Fosfatos de Inositol/metabolismo , NADPH Oxidasas , Ácidos Fosfatidicos/metabolismo , Transducción de SeñalRESUMEN
The role of the activation of phosphoinositide turnover and of the increase in cytosolic free calcium, [Ca2+]i, in the phagocytosis and associated activation of the respiratory burst was investigated. We report the results obtained on the phagocytosis of yeast cells mediated by Con A in normal and in Ca2+-depleted human neutrophils. In normal neutrophils the phagocytosis was associated with a respiratory burst, a stimulation in the formation of [3H] inositol phosphates and [32P]phosphatidic acid, the release of [3H]arachidonic acid, and a rise in [Ca2+]i. Ca2+-depleted neutrophils are able to perform the phagocytosis of yeast cells mediated by Con A and to activate the respiratory burst without stimulation of [3H]inositol phosphates and [32P]phosphatidic acid formation, [3H]arachidonic acid release, and rise in [Ca2+]i. In both normal and Ca2+-depleted neutrophils the phagocytosis and the associated respiratory burst, 1) were inhibited by cytochalasin B; 2) were insensitive to H-7, an inhibitor of protein kinase C; and 3) did not involve GTP-binding protein sensitive to pertussis toxin. These findings indicate that the activation of phosphoinositide turnover, the liberation of arachidonic acid, the rise in [Ca2+]i, and the activity of protein kinase C are not necessarily required for ingestion of Con A-opsonized particles and for associated activation of the NADPH oxidase, the enzyme responsible for the respiratory burst. The molecular mechanisms of these phosphoinositide and Ca2+-independent responses are discussed.
Asunto(s)
Ácidos Araquidónicos/metabolismo , Calcio/biosíntesis , Concanavalina A/farmacología , Neutrófilos/fisiología , Consumo de Oxígeno , Fagocitosis/efectos de los fármacos , Fosfatidilinositoles/metabolismo , Activación Enzimática/efectos de los fármacos , Humanos , Neutrófilos/enzimología , Neutrófilos/metabolismo , Consumo de Oxígeno/efectos de los fármacos , Proteína Quinasa C/metabolismo , Saccharomyces cerevisiae/fisiologíaRESUMEN
The neuropeptide substance P (SP), which has been suggested to mediate neurogenic inflammation, induces in human neutrophils the activation of the respiratory burst measured as O2 consumption and H2O2 production, and a cytochalasin B-dependent secretion of specific and azurophilic granules. The SP(4-11) fragment is much more stimulant than the entire molecule, whereas the SP(1-4) fragment is inactive. The respiratory and secretory response to SP are associated with an activation of phosphoinositide turnover, of Ca2+ influx and release from intracellular stores. Pertussis toxin inhibits 70% of the respiratory response and the residual 30% activity remains, even increasing 10-fold the concentration of the toxin. 1-(5-Isoquinolinesulfonyl)-2-methylpiperazine, a putative inhibitor of protein kinase C, does not modify the respiratory response to SP. Cytochalasin B significantly depresses the activation of the respiration by SP, whereas it moderately enhances the activation of phosphoinositide turnover and potentiates the increase of intracellular Ca2+ concentration. The results are discussed in relation to the receptor apparatus involved in SP activity, the signal transduction sequence activated by SP for the stimulation of NADPH oxidase, and the role of cell response to SP in the inflammatory process.
Asunto(s)
Calcio/metabolismo , Exocitosis/efectos de los fármacos , Fosfatos de Inositol/biosíntesis , Neutrófilos/fisiología , Consumo de Oxígeno/efectos de los fármacos , Sustancia P/farmacología , Fosfatos de Azúcar/biosíntesis , Citosol/metabolismo , Activación Enzimática/efectos de los fármacos , Humanos , Peróxido de Hidrógeno/biosíntesis , NADH NADPH Oxidorreductasas/metabolismo , NADPH Oxidasas , Neutrófilos/enzimología , Neutrófilos/metabolismoRESUMEN
Phosphatidic acid (PA), a molecule that is rapidly produced by the stimulated turnover of phospholipids in a variety of cells including blood neutrophils, elicited NADPH-dependent superoxide anion (O2-) production in detergent extracts from membranes of resting pig neutrophils. The stimulatory effect of PA was independent of cytosolic factors, differing from arachidonic acid and sodium dodecyl sulfate which, on the contrary, absolutely required the presence of cytosol to elicit the same result. The O2(-)-forming activity of the detergent extract activable by PA, as that by sodium dodecyl sulfate and arachidonic acid plus cytosol, was found in the chromatographic fractions containing cytochrome b558 and presented a chromatographic profile identical to that of the activated NADPH oxidase, which was obtained from neutrophils prestimulated with phorbol 12-myristate 13-acetate. The PA-induced NADPH-dependent O2(-)-forming activity showed kinetic properties and sensitivity to the inhibitors similar to the classical ones of the activated neutrophil NADPH oxidase. The data suggest that, in this cell-free system, PA may stimulate O2- formation by direct interaction with latent NADPH oxidase of neutrophils or with some of its regulatory components.
Asunto(s)
NADP/metabolismo , Neutrófilos/metabolismo , Ácidos Fosfatidicos/farmacología , Superóxidos/biosíntesis , Animales , Membrana Celular/metabolismo , Flavina-Adenina Dinucleótido/metabolismo , Cinética , Neutrófilos/efectos de los fármacos , Espectrofotometría , Porcinos , Acetato de Tetradecanoilforbol/farmacologíaRESUMEN
Here we have investigated the ability of recombinant interferon-gamma (rIFN-gamma) to modulate human neutrophil (PMN) functions. PMN incubated in the presence of rIFN-gamma showed an enhanced hydrogen peroxide production in response to Con A, FMLP, PMA or immune complexes. The effect of rIFN-gamma was dose dependent, being half maximal at 2 U/ml, and required between 90 min and 240 min of incubation to reach optimal response. The enhancing effect of rIFN-gamma on the respiratory response of PMN was not blocked by polymixin B sulphate, but an anti-rIFN-gamma monoclonal antibody and cycloheximide and actinomycin D were effective inhibitors. The enhancement of the response to Con A was not accompanied by an enhanced binding of the lectin. Neither the kinetic properties of the Con A-stimulated NADPH oxidase nor the expression of cytochrome b-245 were altered in rIFN-gamma-treated PMN. rIFN-gamma also enhanced granule secretion in response to Con A, FMLP and PMA. Initial studies on the possible alterations of transmembrane signalling in rIFN-gamma-treated PMN showed that neither inositol phosphates formation nor cytoplasmic calcium transients were altered.
Asunto(s)
Exocitosis , Interferón gamma/farmacología , Neutrófilos/fisiología , Oxígeno/sangre , Calcio/sangre , Concanavalina A/sangre , Gránulos Citoplasmáticos/metabolismo , Humanos , Peróxido de Hidrógeno/sangre , Interferón gamma/antagonistas & inhibidores , Monocitos/metabolismo , Neutrófilos/metabolismo , Fosfatidilinositoles/sangre , Proteínas Recombinantes/farmacologíaRESUMEN
Evidences have been provided in our laboratory that in neutrophils different signal transduction sequences for the activation of O2(-)-forming NADPH oxidase can be triggered by the same stimulus (Biochem. Biophys. Res. Commun. 1986, 135, 556-565; 1986, 135, 785-794; 1986, 140, 1-11). The results presented here show that the transduction sequence triggered by fluoride via dissociation of G-proteins and involving messengers produced by stimulation of phosphoinositide turnover, Ca2+ changes and translocation of protein kinase C from the cytosol to the plasmamembrane, can be bypassed when a primed state of neutrophils is previously induced. In fact: i) fluoride causes a pertussis toxin insensitive and H-7 sensitive respiratory burst in human neutrophils, which is linked to the activation of hydrolysis of PIP2, rise in [Ca2+]1 and translocation of PKC. In Ca2+-depleted neutrophils these responses to fluoride do not occur and are restored by addition of CaCl2. ii) The pretreatment of Ca2+-depleted unresponsive neutrophils with non stimulatory doses of PMA restores the activation of the NADPH oxidase by fluoride but not the turnover of phosphoinositides and PKC translocation. The nature of the alternative transduction sequence, the reactions different from phospholipase C activated by G-protein for the alternative sequence and the role of these discrete pathways for NADPH oxidase activation are discussed.
Asunto(s)
Calcio/farmacología , Neutrófilos/metabolismo , Consumo de Oxígeno/efectos de los fármacos , Fosfatidilinositoles/metabolismo , Proteína Quinasa C/metabolismo , Fluoruro de Sodio/farmacología , 1-(5-Isoquinolinesulfonil)-2-Metilpiperazina , Transporte Biológico/efectos de los fármacos , Proteínas de Unión al GTP/metabolismo , Humanos , Isoquinolinas/farmacología , Cinética , N-Formilmetionina Leucil-Fenilalanina/farmacología , NADH NADPH Oxidorreductasas/metabolismo , NADPH Oxidasas , Neutrófilos/efectos de los fármacos , Toxina del Pertussis , Piperazinas/farmacología , Proteína Quinasa C/antagonistas & inhibidores , Acetato de Tetradecanoilforbol/farmacología , Factores de Virulencia de Bordetella/farmacologíaRESUMEN
Zymosan particles opsonised with human serum factors functionally activate human neutrophils and induce a substantial modification of the human neutrophil cytosolic glyoxalase system. The activity of glyoxalase I increases and the activity of glyoxalase II decreases by 20-40% of their resting cell activities during the initial 10 min of activation. The cellular concentration of the glyoxalase intermediate S-D-lactoylglutathione increases by ca. 100% of resting cell levels during this period. This modification may be related to the ability of S-D-lactoylglutathione to stimulate the assembly of microtubules.