RESUMEN
Molecular and histopathological studies were carried out on samples from three Brazilian dogs with persistent hepatozoonosis parasitemia. Canine hepatozoonosis was confirmed by finding gametocytes in blood smears. Histopathological examination of skeletal muscle biopsy revealed muscle degeneration and atrophy, and degenerating/regenerating myofibers, similar to the muscle degeneration caused by Hepatozoon americanum . However, the large cystic structures typical of H. americanum were not observed in muscle biopsies from these dogs. Partial 18S rRNA sequences amplified by PCR from the three Brazilian samples demonstrated closest similarity to the Hepatozoon sp. Fukuoka described in Japan (>99% identity). These results indicate that both additional clinical cases and genes need examination in order to determine the phylogenetic relatedness among canid Hepatozoon species.
Asunto(s)
Coccidios/genética , Coccidiosis/veterinaria , Enfermedades de los Perros/parasitología , ARN Ribosómico 18S/genética , Animales , Secuencia de Bases , Brasil , Coccidiosis/parasitología , Coccidiosis/patología , ADN Protozoario/química , ADN Protozoario/genética , Enfermedades de los Perros/patología , Perros , Femenino , Masculino , Datos de Secuencia Molecular , Músculo Esquelético/parasitología , Músculo Esquelético/patología , Filogenia , Reacción en Cadena de la Polimerasa/veterinaria , Alineación de Secuencia/veterinaria , Análisis de Secuencia de ADN/veterinariaRESUMEN
Canine hepatozoonosis is a disease caused by the tick-borne protozoan Hepatozoon spp. It has been reported in the United States, southern Europe, the Middle East, Africa and the Far East. In Brazil, canine hepatozoonosis is an emerging protozoal tick-borne disease, and is characterized by distinct clinical signs. The objective of this study was to analyze the laboratory findings of some hepatozoonosis cases in dogs in Brasília, Brazil, and their clinical signs. The animals of this experiment showed low parasitemia, similar to H. americanum, but the clinical signs presented were similar to H. canis. According to our observations and in agreement with O'Dwyer et al. [Vet. Parasitol. 94 (2001) 143], the Brazilian Hepatozoon appears more to resemble the species found in the eastern Hemisphere than with H. americanum of North America, or could be caused by a new species. Our data revealed that hepatozoonosis could be considered endemic in Brasília.
Asunto(s)
Coccidios/crecimiento & desarrollo , Coccidiosis/veterinaria , Enfermedades de los Perros/parasitología , Animales , Brasil , Coccidiosis/sangre , Coccidiosis/parasitología , Perros , Recuento de Eritrocitos/veterinaria , Hematócrito/veterinaria , Hemoglobinas/metabolismo , Recuento de Leucocitos/veterinaria , Recuento de Linfocitos/veterinariaRESUMEN
Amostras de sangue de 106 cäes de rua da cidade de Säo Paulo foram examinadas para pesquisa de Babesia canis. As técnicas utilizadas foram a da observaçäo direta do parasita em esfregaço sangüíneo, corado por corante de Rosenfeld, e a reaçäo de imunofluorescência indireta (IFI). Através da primeira diagnosticou-se a infecçäo em 10,3% dos animais, enquanto que a sorologia revelou 42,4% de cäes positivos. No teste de IFI os títulos variaram de 1:10 a 1:2560, sendo mais frequentes o de 1:40. Este último teste apresentou boa sensibilidade e alta especificidade, mostrando-se ideal para levantamentos epidemiológicos.
Asunto(s)
Perros , Animales , Babesiosis/diagnósticoRESUMEN
Estudos morfológicos de 35 ascarideos, colhidos de abcesso fistulado do pescoço de um gato doméstico, permitiram diagnóstica-los como Lagochilascaris major Leiper, 1910. As medidas e número de escavaçöes presentes na superfície dos ovos se assemelharam aquelas verificadas para L. sprenti (BOWMAN et alii, 2, 1983 e para L. major (AMATO et alii, 1, 1982)