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1.
Invest Clin ; 38(2): 95-106, 1997 Jun.
Artículo en Español | MEDLINE | ID: mdl-9296644

RESUMEN

In 1964, Pfeiffer described a syndrome consisting of craniosynostosis, broad thumbs, broad great toes, and partial soft tissue syndactyly of the hands and feet. It belongs to acrocephalosyndactyly syndromes. We describe a male baby product of an eighth full-term uncomplicated uncontrolled pregnancy, mother and father normal and unrelated, 32 and 50 years old, respectively. He had all diagnostic and prognostic criteria of Subtype 2 Pfeiffer's Syndrome. The clinical, radiological, tomographic, and genetic aspects are discussed.


Asunto(s)
Acrocefalosindactilia , Acrocefalosindactilia/clasificación , Acrocefalosindactilia/diagnóstico por imagen , Acrocefalosindactilia/genética , Adulto , Femenino , Humanos , Recién Nacido , Masculino , Radiografía , Venezuela
2.
Invest Clin ; 38(3): 145-53, 1997 Sep.
Artículo en Español | MEDLINE | ID: mdl-9376420

RESUMEN

Cystic Fibrosis (CF) is a severe and relatively common autosomic recessive disease caused by a variety of mutations in the CFTR gene. The most frequent mutation worldwide, consists of the deletion of the phenylalanine codon at position 508 (delta F508). Here we report the first cases of prenatal diagnosis of CF by DNA analysis in couples at risk in Venezuela. The study focused on the detection of delta F508 alleles analyzing DNA recovered directly from amniocytes or from their cultures, using the polymerase chain reaction (PCR) and polyacrylamide gel electrophoresis. Two of three fetuses resulted homozygotic for the delta F508 allele and the third one turned out to be a delta F508 carrier. This information sustained the genetic counseling of the couples and allowed them to take objective reproductive decisions, a direct consequence of the availability of gene analysis at the DNA level.


Asunto(s)
Amniocentesis , Regulador de Conductancia de Transmembrana de Fibrosis Quística/genética , Fibrosis Quística/diagnóstico , Análisis Mutacional de ADN , Enfermedades Fetales/diagnóstico , Eliminación de Secuencia , Adulto , Alelos , Codón/genética , Fibrosis Quística/embriología , Femenino , Humanos , Masculino , Embarazo
3.
Invest Clin ; 38(4): 219-26, 1997 Dec.
Artículo en Español | MEDLINE | ID: mdl-9527389

RESUMEN

Werner in 1915, described a patient is characterized by a tibial bilateral aplasia or hypoplasia, polydactyly and absent thumbs. Autosomal dominant inheritance is demonstrated, with variable expressivity. The objective of this work is to describe a child with clinic and radiologic signs of Tibial Hypoplasia with Polydactyly. The genealogic study allowed us to suppose that the gene has a variable expressivity, since in the maternal branch, malformations such as syndactyly of hands, proximal implantation of thumbs and tibiae vara, have been found. The clinic, radiologic, and genetic aspects are discussed.


Asunto(s)
Anomalías Múltiples/genética , Polidactilia/genética , Pulgar/anomalías , Tibia/anomalías , Femenino , Deformidades Congénitas del Pie/genética , Luxación Congénita de la Cadera/genética , Humanos , Recién Nacido , Linaje , Estenosis Pilórica/congénito , Sindactilia/genética , Venezuela
4.
Invest Clin ; 35(4): 195-207, 1994 Dec.
Artículo en Español | MEDLINE | ID: mdl-7734522

RESUMEN

Duchenne and Becker muscular dystrophy (DMD/BMD) are recessive X-linked neuromuscular diseases produced by allelic mutations in the human dystrophin gen. In the present study we determined the 14-deletion prone exons by multiplex PCR in 24 no related venezuelan patients with clinical diagnosis of DMD/BMD. We found 37% of intragenic deletions of which 77% were located at the "hot spot" deletion region that includes exons 44 to 55. The present study show that deletion frequency observed in venezuelan patients resembles some Asian populations and is lower than that observed in Europe and North America. The explanation of the low frequency detected in our patients is beyond the present study, but it is likely that different mutations, ocurring at other regions of the gene is determining a molecular heterogeneity of the DMD/BMD disease in Venezuela.


Asunto(s)
Distrofias Musculares/diagnóstico , Reacción en Cadena de la Polimerasa , Deleción Cromosómica , Mapeo Cromosómico , Humanos , Masculino , Distrofias Musculares/genética , Venezuela
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