RESUMEN
Because exacerbation of colitis seems to be associated with stress, we proposed evaluating the influence of stress and the involvement of corticotropin-releasing factor (CRF) and arginine vasopressin (AVP) on experimental colitis in rats. Partial restraint stress was applied during 4 consecutive days, before or after intracolonic 2,4,6-trinitrobenzenesulfonic acid (TNB) instillation (15 mg) in rats. Finally, two groups of rats were centrally injected with alpha-helical CRF-(9-41) (5 micrograms) or AVP antagonist (5 micrograms) before each session of stress. Stress was applied before or right after TNB enhanced colitis, with an increase in macroscopic and histological scores and myeloperoxidase activity, alpha-Helical CRF-(9-41) or AVP antagonist had no effect on TNB-induced colitis but enhanced the effects of stress on colitis. These results show that stress may exacerbate experimental colitis in rats and that CRF and AVP are not responsible for this effect.
Asunto(s)
Arginina Vasopresina/fisiología , Colitis/complicaciones , Colitis/patología , Hormona Liberadora de Corticotropina/fisiología , Estrés Fisiológico/complicaciones , Animales , Arginina Vasopresina/antagonistas & inhibidores , Colitis/inducido químicamente , Colon/enzimología , Colon/patología , Hormona Liberadora de Corticotropina/farmacología , Antagonistas de Hormonas/farmacología , Inyecciones Intraventriculares , Mucosa Intestinal/enzimología , Mucosa Intestinal/patología , Masculino , Fragmentos de Péptidos/farmacología , Peroxidasa/metabolismo , Ratas , Ratas Wistar , Restricción Física , Ácido TrinitrobencenosulfónicoRESUMEN
BACKGROUND: Psychological factors have long been implicated in the aetiology of irritable bowel syndrome often associated with abdominal pain. This work was designed to study, in rats, the influence of partial restraint stress on the abdominal cramps induced by rectal distension and to determine the role of corticotropin releasing factor (CRF) and mast cells degranulation in this response. METHODS: Abdominal contractions were electromyographically recorded. Thirty minutes after stress or intracerebroventricular CRF, rectal distension was performed by inflation of a balloon (0.4-1.2 mL). alpha-helical CRF9-41 or doxantrazole were administered centrally (15 min) and intraperitoneally (30 min), respectively, before stress. Histamine release and the number of mast cells were determined in colonic pieces from stressed and control rats. RESULTS: Stress and CRF enhanced the number of abdominal cramps evoked by rectal distension without affecting rectal compliance. alpha-helical CRF9-41 and doxantrazole antagonized the stress and CRF-induced enhancement of abdominal cramps. Stress increased the colonic histamine content whereas the number of colonic mast cells was unchanged. CONCLUSIONS: Stress enhances abdominal contractions in response to rectal distension in rats via pathways involving central CRF and intestinal mast cells.
Asunto(s)
Ventrículos Cerebrales/fisiología , Colon/fisiopatología , Hormona Liberadora de Corticotropina/farmacología , Liberación de Histamina , Mastocitos/fisiología , Fragmentos de Péptidos/farmacología , Recto/fisiopatología , Estrés Psicológico/fisiopatología , Animales , Artefactos , Ventrículos Cerebrales/efectos de los fármacos , Ventrículos Cerebrales/fisiopatología , Cólico , Colon/efectos de los fármacos , Colon/fisiología , Hormona Liberadora de Corticotropina/administración & dosificación , Electromiografía , Antagonistas de Hormonas/administración & dosificación , Antagonistas de Hormonas/farmacología , Inyecciones Intraperitoneales , Inyecciones Intraventriculares , Masculino , Fragmentos de Péptidos/administración & dosificación , Ratas , Ratas Wistar , Recto/efectos de los fármacos , Recto/fisiología , Restricción Física , Tioxantenos/administración & dosificación , Tioxantenos/farmacología , XantonasRESUMEN
1. The role of dopamine in the genesis of corticotropin releasing factor (CRF)- and emotional stress (ES)-induced stimulation of colonic motility, as well as the mechanism of antagonistic action of cholecystokinin octapeptide (CCK-8s) and igmesine (alpha sigma receptor ligand, formerly JO 1784) on dopamine-induced colonic hypermotility, have been investigated in the rat. 2. ES and i.c.v. injection of CRF (0.5 microgram kg-1) increased the frequency of colonic spike bursts by 63% and 114%, respectively. Prior i.c.v. administration of (+)-SCH 23390 (a D1 receptor antagonist, 10 micrograms kg-1) significantly (P < 0.05) reduced the CRF- and ES-induced increase in colonic spike burst; whereas, sulpiride (a D2 receptor antagonist, 10 micrograms kg-1) blocked the CRF-induced stimulation of colonic spike bursts but had no effect on the colonic response to stress. 3. I.c.v. injection of dopamine (100 micrograms kg-1), increased colonic spike burst frequency by 54%. (+)-SKF 38393 (5 micrograms kg-1), a selective D1 receptor agonist, and quinpirole (5 micrograms kg-1), a selective D2 receptor agonist, increased colonic spike burst frequency by 71% and 70% respectively. CCK-8s (0.1 microgram kg-1) and igmesine (0.1 microgram kg-1) injected i.c.v. completely prevented the stimulatory effects of dopamine, (+)-SKF 38393 and quinpirole. 4. Previous i.c.v. injection of devazepide, a CCKA receptor antagonist, (10 micrograms kg-1) antagonized the inhibitory effects of both CCK-8s and igmesine injected i.c.v. on dopamine-induced colonic hyperkinesia. 5. These results show that CRF stimulates colonic motility through activation of central dopaminergic mechanisms in response to stress; furthermore, CCK-8s inhibits dopamine-induced colonic hyperkinesia through a mechanism involving D1 and D2 receptors. The sigma receptor ligand igmesine, blocks the CRF and ES-induced colonic hyperactivity via an interaction with central CCK mechanisms.