RESUMEN
BACKGROUND: Physiopathology and prognosis of peptic ulcer bleeding (PUB) have never been described in cirrhotic patients. AIM: To assess risk factors and outcome of PUB in two groups of patients with PUB with or without cirrhosis. METHODS: We included prospectively all patients with PUB referred to our ICU of Hepatology and Gastroenterology between January 2008 and March 2011. All patients were treated according to international recommendations. Diagnosis of cirrhosis was based on clinical, biological and morphological exams. Aetiologies, characteristics and outcomes of PUB were compared in cirrhotic vs. noncirrhotic patients. RESULTS: A total of 203 patients with PUB were included prospectively. Twenty-nine patients had cirrhosis (group Cirr+), and 174 patients had no cirrhosis (group Cirr-). Demographic data were similar between the two groups except for age and alcohol consumption. Aetiology of cirrhosis was alcohol in 97% of cirrhotic patients. Characteristics of PUB were not different between the two groups. Ninety-three per cent of patients with cirrhosis had endoscopic portal hypertension. Aetiology of PUB was different between the group Cirr+ and Cirr- (Helicobacter pylori = 10.3% vs. 48.8%, P < 0.0001; NSAID's = 17.2% vs. 54.0%, P < 0.0001; idiopathic PUB = 79.3% vs. 23.8%, P < 0.0001). Outcome was comparable concerning re-bleeding (7.0% vs. 6.9%, P = 0.31), need for arterial embolisation (10.3 vs. 8.6%, P = 0.76), need for salvage surgery (0 vs. 1.7%, P = 0.31) and mortality (3.0% vs. 1.1%, P = 0.87). CONCLUSIONS: Physiopathology of PUB seems to be different in patients with cirrhosis. In cirrhotic patients, PUB occurs almost only in alcoholics. In our series, prognosis was similar to general population. PUB in cirrhosis might be related to portal hypertension and/or alcohol.
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Consumo de Bebidas Alcohólicas/efectos adversos , Antiinflamatorios no Esteroideos/efectos adversos , Infecciones por Helicobacter/complicaciones , Cirrosis Hepática/complicaciones , Úlcera Péptica Hemorrágica/etiología , Femenino , Helicobacter pylori/aislamiento & purificación , Humanos , Cirrosis Hepática/tratamiento farmacológico , Cirrosis Hepática/fisiopatología , Masculino , Persona de Mediana Edad , Úlcera Péptica Hemorrágica/tratamiento farmacológico , Úlcera Péptica Hemorrágica/fisiopatología , Pronóstico , Estudios Prospectivos , Factores de RiesgoRESUMEN
Nonsteroidal anti-inflammatory drugs (NSAIDs), other than aspirin, are to some extent metabolized by cytochrome P450 2C9 (CYP2C9). The CYP2C9 359Leu (CYP2C9*3) loss-of-function allele could be a risk factor for acute upper gastrointestinal bleeding (AUGIB) related to the use of NSAIDs other than aspirin. To test this hypothesis, we performed a prospective, multicenter, case-case study in patients hospitalized for AUGIB related to the use of NSAIDs. A total of 131 patients had been treated with aspirin and 57 patients had been treated with an NSAID other than aspirin (non-ASP). In the aspirin group, 12 patients (9.2%) had the CYP2C9 359Leu allele as compared with 19 (33.3%) in the non-ASP group (odds ratio (OR) = 5.0; 95% confidence interval 2.2-11.1, P < 0.0001). In a multivariate analysis, CYP2C9 359Leu remained associated with the non-ASP group (OR = 7.2 (2.6-20.3), P = 0.0002) even though 40% of these patients were under treatment with antiulcer drugs at the time of admission. Therefore the results of the study support the hypothesis that the CYP2C9 359Leu allele is a robust risk factor for AUGIB related to the use of NSAIDs other than aspirin.
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Antiinflamatorios no Esteroideos/efectos adversos , Hidrocarburo de Aril Hidroxilasas/genética , Aspirina/efectos adversos , Hemorragia Gastrointestinal/inducido químicamente , Anciano , Anciano de 80 o más Años , Alelos , Antiinflamatorios no Esteroideos/metabolismo , Antiulcerosos/uso terapéutico , Estudios de Casos y Controles , Citocromo P-450 CYP2C9 , Femenino , Hemorragia Gastrointestinal/genética , Humanos , Masculino , Persona de Mediana Edad , Análisis Multivariante , Estudios Prospectivos , Factores de RiesgoRESUMEN
AIM OF THE STUDY: Diagnosis of Clostridium difficile-associated diarrhea is classically based on detection of toxin A and/or toxins A+B by using several techniques. However, these techniques show important differences in terms of sensitivity and specificity. In this work, we compared three commercial immunoenzymatic tests for detecting C. difficile toxins. METHODS: We used three immunoenzymatic techniques: ELISA Premiertrade mark Toxins A and B (Meridian), ImmunoCard (IC) Toxins A and B (Meridian), and Triage (TRI) Antigen GDH and Toxin A (Biosite). Culture on the specific media C. difficile (bioMérieux) was performed in parallel. RESULTS: During two years, 898 stool specimens have been tested by using the three different techniques. According to the manufacturer's recommendations (sample positive if optical density [OD] value greater or equal to 0.15), 205 (22.8%) were positive for ELISA. Among them, 65 (31.7%) were negative for all other tests, and they have been considered as false-positive results. This discrepancy led us to choose other OD values (greater than 0.75: positive result; 0.15-0.75: limit result). For limit results, IC, TRI, antigen GDH, and culture methods were positive in 30, 2, 41, and 29% of cases, respectively, whereas for positive results (>0.75), they were positive in 82, 54, 84, and 76% of cases, respectively. CONCLUSION: ELISA and IC tests are the most powerful and are concordant together if interpretation is performed with OD values redefined by the microbiology laboratory. The choice of the technique to use depends on the number of samples to analyze, the rapidity of the result, and the cost.
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Proteínas Bacterianas/análisis , Toxinas Bacterianas/análisis , Clostridioides difficile/química , Enterotoxinas/análisis , Técnicas para Inmunoenzimas/métodos , Juego de Reactivos para Diagnóstico , Antígenos Bacterianos/análisis , Clostridioides difficile/crecimiento & desarrollo , Clostridioides difficile/inmunología , Clostridioides difficile/aislamiento & purificación , Infección Hospitalaria/microbiología , Medios de Cultivo , Enterocolitis Seudomembranosa/diagnóstico , Enterocolitis Seudomembranosa/microbiología , Ensayo de Inmunoadsorción Enzimática/métodos , Reacciones Falso Positivas , Heces/microbiología , Humanos , Sensibilidad y EspecificidadRESUMEN
INTRODUCTION: Cat-Scratch Disease (CSD) is a well-recognized benign cause of localized lymphadenopathy, which often recovers spontaneously. However systemic clinical presentations are described in immunodeficient adults (bacillary angiomatosis, bacillary splenitis) and are less common in immunocompetent ones. EXEGESIS: We report two cases of disseminated CSD in immunocompetent patients, presenting hepatosplenic nodules, associated in the second case with an endocarditis. CONCLUSION: Bartonella serology must be achieved in case of hepatosplenic nodules with fever. Treatment of disseminated CSD in immunocompetent adults is still empirical and recovery can occur without antibiotherapy when endocarditis is not associated.
Asunto(s)
Infecciones por Bartonella/diagnóstico , Enfermedad por Rasguño de Gato/diagnóstico , Endocarditis Bacteriana/microbiología , Inmunocompetencia , Absceso Hepático/microbiología , Enfermedades del Bazo/microbiología , Anciano , Animales , Antibacterianos/uso terapéutico , Infecciones por Bartonella/tratamiento farmacológico , Infecciones por Bartonella/microbiología , Bartonella henselae/aislamiento & purificación , Enfermedad por Rasguño de Gato/tratamiento farmacológico , Enfermedad por Rasguño de Gato/microbiología , Gatos , Endocarditis Bacteriana/diagnóstico , Endocarditis Bacteriana/tratamiento farmacológico , Femenino , Humanos , Absceso Hepático/diagnóstico , Absceso Hepático/tratamiento farmacológico , Masculino , Persona de Mediana Edad , Enfermedades del Bazo/diagnóstico , Enfermedades del Bazo/tratamiento farmacológico , Resultado del TratamientoRESUMEN
AIM OF THE STUDY: Bacteriological confirmation of pneumonia (PNM) in hospitalized patients is often erratic or belated. Because of importance of prognosis, early adaptation of treatment requires an empirical antimicrobial therapy (generally aminopenicillin and macrolide combination). The starting therapeutic strategy should profit by a fast and reliable test asserting a pneumococcal etiology. The Binax Now S. pneumoniae (BNP) test allows an urinary pneumococcal antigen (UPA) detection using an immunochromatographic membrane assay within 15 minutes. MATERIALS AND METHODS: We first evaluated the BNP test for 28 patients with pneumococcal PNM proved by culture, and 118 negative control patients without PNM. The BNP test was then evaluated by testing urine from 158 hospitalized patients with a clinical picture of PNM (community-acquired: 90, nosocomial: 68) for whom a research of urinary Legionella antigen (Binax Now) was prescribed and was positive for only two cases. 57 patients (36.1%) were hospitalized in ICU. RESULTS: The sensitivity was 71.4% (85.7% for the 21 bacteriemic PNM), and the specificity was 98.3%; that is consistent with previous published data. Among the 158 patients with PNM, UPA was detected in 17 cases (10.8%): 15 within the community-acquired PNM (16.7%) and 2 (2.9%) within the nosocomial cases. The pneumococcal etiology was confirmed by bacteriological samples in 7/17 patients (6 by blood cultures). The 10 others showed clinical and radiological features in agreement with a pneumococcal PNM. Among the 141 patients with negative AUP, S. pneumoniae was isolated from 6 of them (2 in blood cultures). CONCLUSION: The Binax Now S. pneumoniae test allowed a fast and reliable etiological diagnosis in 10.8% of hospitalized PNM (16.7% of the community-acquired cases) having a research of urinary Legionella antigen (conceiving with severity factors). So it could conduce to an improved adjustment of the starting antimicrobial therapy of hospitalized adult patients with PNM.
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Antígenos Bacterianos/orina , Pacientes Internos , Legionella/aislamiento & purificación , Infecciones Neumocócicas/diagnóstico , Neumonía Neumocócica/diagnóstico , Streptococcus pneumoniae/aislamiento & purificación , Antígenos Bacterianos/análisis , Infecciones Comunitarias Adquiridas/diagnóstico , Infecciones Comunitarias Adquiridas/orina , Humanos , Infecciones Neumocócicas/orina , Neumonía Neumocócica/orina , Estudios Prospectivos , Valores de ReferenciaRESUMEN
We report the first isolation of Mycobacterium microti from a dog with lesions of acute peritonitis. The isolate was demonstrated to be M. microti of Llama-Type by spoligotyping. Epidemiological implications of the isolation of this possibly zoonotic agent from a dog are discussed.
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Enfermedades de los Perros/microbiología , Mycobacterium/aislamiento & purificación , Peritonitis/veterinaria , Enfermedad Aguda , Animales , Biopsia con Aguja Fina/veterinaria , Enfermedades de los Perros/transmisión , Perros , Resultado Fatal , Humanos , Masculino , Mycobacterium/clasificación , Peritonitis/microbiología , ZoonosisRESUMEN
Bartonella species are emerging as an important cause of blood culture-negative endocarditis, but the optimal management of this disease has not been fully defined. We describe a case of subacute Bartonella henselae endocarditis of a prosthetic aortic valve in an immunocompetent woman that was cured with long-term antibiotic therapy alone. In addition, we demonstrate that follow-up of serologic titers against B. henselae was helpful in assessing definitive cure of the infection.
Asunto(s)
Antibacterianos/uso terapéutico , Anticuerpos Antibacterianos/sangre , Válvula Aórtica/microbiología , Bartonella henselae/efectos de los fármacos , Endocarditis Bacteriana/tratamiento farmacológico , Prótesis Valvulares Cardíacas/microbiología , Anciano , Angiomatosis Bacilar/tratamiento farmacológico , Angiomatosis Bacilar/microbiología , Bartonella henselae/inmunología , Bartonella henselae/aislamiento & purificación , Endocarditis Bacteriana/microbiología , Femenino , Humanos , Infecciones Relacionadas con Prótesis/tratamiento farmacológico , Infecciones Relacionadas con Prótesis/microbiología , Resultado del TratamientoRESUMEN
OBJECTIVE: Gastric lymphoma of mucosa-associated lymphoid tissue (MALT) type is closely related to Helicobacter pylori (H. pylori) infection. In vitro studies have demonstrated H. pylori-induced B cell proliferation to be strain dependent. High prevalences of CagA protein and FldA protein have been reported in strains obtained from patients with gastric lymphoma of MALT type. The aims of the present study were to evaluate the prevalence of H. pylori infection and to search for antigenic particularities in 53 patients with primary gastric lymphoma in comparison with a group of infected patients with benign disease. METHODS: Of the 53 patients, 37 presented with low-grade lymphoma of MALT type (LGLM) and 16 with diffuse large B-cell lymphoma (DLBCL). They were compared to a group of 162 H. pylori-infected subjects comprising the control group: 111 had gastric or duodenal ulcer (GDU) and 51 nonulcer dyspepsia (NUD). Diagnosis of gastric lymphoma was established on histological examination of endoscopic specimens. Anti-H. pylori antibodies were assayed by third-generation ELISA. Western blot assay was used to detect antibodies against nine antigens (including CagA protein), which were recognized on the basis of their molecular weight. RESULTS: Of the 53 patients with gastric lymphoma, 45 were H. pylori-positive (85%): of these, 25 (56.5%) had anti-CagA antibodies. The prevalence of H. pylori seropositivity was 78% (29/37) in LGLM and 100% (16/16) in DLBCL. The prevalence of CagA seropositivity in H. pylori-positive patients was 44.8% (13/29) and 75% (12/16), respectively (p < 0.05). In comparison, the seroprevalence of CagA was 77.4% (86/111) in GDU patients and 43.1% (22/53) in NUD patients. The prevalence of antibodies to other antigenic proteins detected with Helicoblot 2.0 (19.5kd, 30kd, 35kd, VacA, HSPb, Urease A, and Urease B) did not differ among the groups except for 35kd protein, which was significantly higher (p < 0.01) in GDU than in NUD and in LGLM (76.6% vs 49% and 46.7%). CONCLUSION: These findings suggest that in patients who develop gastric lymphomas in response to H. pylori, virulent strains expressing CagA protein are preferentially associated with DLBCL.
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Antígenos Bacterianos , Proteínas Bacterianas/sangre , Infecciones por Helicobacter/sangre , Helicobacter pylori , Linfoma de Células B de la Zona Marginal/microbiología , Linfoma de Células B/microbiología , Linfoma de Células B Grandes Difuso/microbiología , Neoplasias Gástricas/microbiología , Análisis de Varianza , Antineoplásicos/uso terapéutico , Western Blotting , Distribución de Chi-Cuadrado , Femenino , Infecciones por Helicobacter/tratamiento farmacológico , Infecciones por Helicobacter/epidemiología , Humanos , Linfoma de Células B/sangre , Linfoma de Células B/tratamiento farmacológico , Linfoma de Células B/patología , Linfoma de Células B de la Zona Marginal/tratamiento farmacológico , Linfoma de Células B de la Zona Marginal/patología , Linfoma de Células B Grandes Difuso/sangre , Linfoma de Células B Grandes Difuso/tratamiento farmacológico , Linfoma de Células B Grandes Difuso/patología , Masculino , Persona de Mediana Edad , Estudios Seroepidemiológicos , Neoplasias Gástricas/sangre , Neoplasias Gástricas/tratamiento farmacológico , Neoplasias Gástricas/patologíaAsunto(s)
Angiomatosis Bacilar , Endocarditis Bacteriana/epidemiología , Endocarditis Bacteriana/microbiología , Fiebre de las Trincheras , Adolescente , Adulto , Anciano , Aminoglicósidos , Antibacterianos/uso terapéutico , Estudios de Casos y Controles , Quimioterapia Combinada/uso terapéutico , Endocarditis Bacteriana/tratamiento farmacológico , Femenino , Francia , Humanos , Incidencia , Lactamas/uso terapéutico , Masculino , Persona de Mediana EdadRESUMEN
BACKGROUND: It has been suggested that Helicobacter pylori may induce more or less severe gastroduodenal disease according to the strain virulence. DESIGN: We used Western blot to determine antigenic profiles associated with duodenal or gastric ulcer disease, MALT lymphoma and non-ulcer dyspepsia, and to identify geographical differences. METHODS: One hundred and eighty-two consecutive patients with H. pylori infection were studied. H. pylori infection was diagnosed by a rapid urease test or histological examination of gastric biopsy samples. Bacterial density and gastritis were assessed histologically by using the Sydney scoring system. Western blot was used to identify antibodies against eight antigens (CagA, VacA, urease A, heat shock protein B, and 19.5, 26.5, 30 and 35 kDa). Patients were questioned on their smoking habits and place of birth and childhood. RESULTS: There were 73 patients with duodenal ulcer, 30 with gastric ulcer, eight with erosive duodenitis, 17 with gastric MALT lymphoma and 54 with non-ulcer dyspepsia. Most (>85%) were seropositive for the heat shock protein B and 26.5-kDa antigens. The prevalence of the other antigens ranged from 45% (VacA) to 68% (urease B). The seroprevalence of CagA antigen was significantly higher (P < 0.01) in cases of gastroduodenal ulcer (84%) than non-ulcer dyspepsia (37%). Similarly, 35-kDa antigen reactivity was more frequent (P < 0.05) in duodenal ulcer patients (75%) than in those with non-ulcer dyspepsia (50%). The antigenic profiles associated with MALT gastric lymphoma and non-ulcer dyspepsia were similar. Multivariate analysis showed that only gastroduodenal ulcer was significantly associated with CagA. Gastroduodenal ulcer and a childhood spent in Africa were both associated with 35-kDa and combined CagA-35-kDa reactivity. CONCLUSIONS: This study confirms the strong seroprevalence of H. pylori CagA antigen and shows a high prevalence of the 35-kDa antigen in patients with gastroduodenal ulcer, especially those raised in Africa. There was no difference in the serological pattern between patients with non-ulcer dyspepsia and those with MALT lymphoma. Tests for antibodies to the CagA-35-kDa antigen combination might be used to select H. pylori-infected dyspeptic patients warranting treatment.
Asunto(s)
Antígenos Bacterianos/sangre , Dispepsia/inmunología , Infecciones por Helicobacter/inmunología , Helicobacter pylori/inmunología , Linfoma de Células B de la Zona Marginal/inmunología , Úlcera Péptica/inmunología , Neoplasias Gástricas/inmunología , África/epidemiología , Aldehído Reductasa , Anticuerpos Antibacterianos/análisis , Proteínas Bacterianas/inmunología , Western Blotting , Úlcera Duodenal/epidemiología , Úlcera Duodenal/inmunología , Úlcera Duodenal/microbiología , Dispepsia/microbiología , Europa (Continente)/epidemiología , Genes Bacterianos , Proteínas de Choque Térmico/inmunología , Infecciones por Helicobacter/epidemiología , Humanos , Linfoma de Células B de la Zona Marginal/microbiología , Úlcera Péptica/epidemiología , Úlcera Péptica/microbiología , Estudios Seroepidemiológicos , Estadísticas no Paramétricas , Neoplasias Gástricas/microbiología , Úlcera Gástrica/epidemiología , Úlcera Gástrica/inmunología , Úlcera Gástrica/microbiologíaRESUMEN
BACKGROUND: Skin manifestations have been described in 25% of patients with Mycoplasma pneumoniae infection. CASE REPORT: We report a case of Mycoplasma pneumoniae infection in a 29-year-old man who developed a polymorphous erythema-like reaction. Skin manifestations were associated with voluminous lymph node enlargement and high eosinophil levels both in serum and alveolar lavage. Seroconversion against Mycoplasma pneumoniae IgG was documented with ELISA. The clinical course was favorable with erythromycin. DISCUSSION: ELISA IgG seroconversion is sufficient to confirm the diagnosis of Mycoplasma pneumoniae infection as this test has an 80-90% specificity. This case was unusual by its clinical presentation and high eosinophil counts in serum and tissue samples, similar to what is found in drug-induced hypersensitivity.
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Dermatitis Atópica/diagnóstico , Mycoplasma pneumoniae/inmunología , Neumonía por Mycoplasma/diagnóstico , Adulto , Anticuerpos Antibacterianos/sangre , Dermatitis Atópica/inmunología , Diagnóstico Diferencial , Ensayo de Inmunoadsorción Enzimática , Eosinofilia/diagnóstico , Eosinofilia/inmunología , Humanos , Inmunoglobulina G/sangre , Masculino , Neumonía por Mycoplasma/inmunologíaRESUMEN
In investigating a possible link between a novel retroviral agent (provisionally called MSRV), recently characterised in multiple sclerosis (MS), and the neuropathology of MS, it was found that there was a significant correlation between gliotoxicity and reverse transcriptase activity in monocyte/macrophage culture supernatants (MMCS) unique to MS patients. MMCS from healthy controls and patients with other neurological diseases did not display either gliotoxicity or reverse transcriptase activity. The observed gliotoxic effect was an initial, intermediate filament network disorganization and subsequent cell death which was specific to astrocytes and oligodendrocytes. The reverse transcriptase activity and MSRV-specific RNA were observed during the first 2 weeks of culture in MMCS from patients with active MS. The further elucidation of the molecular form(s) of this gliotoxic factor and its original source may be crucial in elucidating important etiopathogenic mechanisms in MS.
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Macrófagos/patología , Monocitos/patología , Esclerosis Múltiple/sangre , Esclerosis Múltiple/virología , Neurotoxinas/aislamiento & purificación , ARN Viral/aislamiento & purificación , ADN Polimerasa Dirigida por ARN/aislamiento & purificación , Retroviridae/aislamiento & purificación , Animales , Astrocitos/citología , Astrocitos/patología , Línea Celular Transformada , Células Cultivadas , Corteza Cerebral/citología , Medios de Cultivo , Feto , Humanos , Macrófagos/citología , Macrófagos/virología , Monocitos/citología , Monocitos/virología , Neurotoxinas/toxicidad , Oligodendroglía/citología , Oligodendroglía/patología , Proteínas/aislamiento & purificación , Proteínas/toxicidad , Ratas , Ratas Wistar , Retroviridae/enzimología , Retroviridae/genéticaRESUMEN
OBJECTIVE: To investigate the immunogenicity of two recombinant hepatitis B vaccines containing S antigen alone (Engerix B) or both S and pre-S2 antigens (GenHevac B) in diabetic patients. RESEARCH DESIGN AND METHODS: Of the adult diabetic patients, 71 (26 IDDM, 45 NIDDM) were randomized to receive Engerix B or GenHevac B at 0, 1, 2, and 12 months in a single-blind clinical trial; if the antibody to hepatitis B surface antigen (anti-HBs) titers were < 10 i.u./l at month 4, a fourth injection of vaccine was given. A positive response was defined by anti-HBs titer > or = 10 IU/l at month 13. RESULTS: The anti-HBs response rate and the titers of anti-HBs did not differ significantly between the two types of vaccine. Overall, > 90% of the patients responded at month 13. In patients vaccinated with GenHevac B, anti-pre-S2 antibodies appeared earlier than anti-HBs. The anti-HBs response tended to decrease with age (P = 0.07) and tended to be higher in IDDM patients than in NIDDM patients (P = 0.06). Metabolic control, as assessed by HbA1c level, did not influence the response rate. The presence of the HLA DQ2 allele was associated with a low response. CONCLUSIONS: A large majority of diabetic patients can be efficiently vaccinated against the hepatitis B virus using a booster dose at month 4. The choice of the vaccine (with or without pre-S2 antigen) appears to have little influence, if any, on the response rate.
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Diabetes Mellitus/inmunología , Anticuerpos contra la Hepatitis B/sangre , Antígenos de Superficie de la Hepatitis B/inmunología , Vacunas contra Hepatitis B/inmunología , Hepatitis B/prevención & control , Precursores de Proteínas/inmunología , Vacunas Sintéticas/inmunología , Adulto , Anciano , Estudios de Cohortes , Diabetes Mellitus/fisiopatología , Ensayo de Inmunoadsorción Enzimática , Femenino , Hepatitis B/inmunología , Anticuerpos contra la Hepatitis B/inmunología , Antígenos de Superficie de la Hepatitis B/administración & dosificación , Vacunas contra Hepatitis B/química , Humanos , Masculino , Persona de Mediana Edad , Precursores de Proteínas/administración & dosificación , Factores de Tiempo , Vacunación/métodos , Vacunas Sintéticas/química , Proteínas del Envoltorio Viral/administración & dosificación , Proteínas del Envoltorio Viral/inmunologíaRESUMEN
OBJECTIVES: To compare the expression of endogenous retroviruses in MS patients and controls. MATERIAL AND METHODS: Peripheral blood mononuclear cells were obtained from 22 MS patients, a corresponding number of matched healthy donors and five patients with other central nervous system disease. Also brain specimens from MS patients and controls were obtained. Transcripts of various endogenous retroviruses in these samples were detected by RNA-PCR. RESULTS: Several endogenous retroviral sequences were transcribed in peripheral blood mononuclear cells and brain tissue from MS patients as well as controls. A composite transcript of an endogenous retrovirus and a zinc finger sequence was more frequently found in healthy donors than in MS patients. CONCLUSION: Some endogenous retroviruses are normally transcribed in white blood cells and brain tissue. The significance of those findings, which concerned the composite transcripts of the zinc finger sequence and its associated endogenous retrovirus is uncertain.
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Esclerosis Múltiple/virología , Retroviridae/genética , Adulto , Encéfalo/virología , Femenino , Regulación Viral de la Expresión Génica/fisiología , Humanos , Masculino , Persona de Mediana Edad , Monocitos/virología , Reacción en Cadena de la Polimerasa , Infecciones por Retroviridae/virología , Transcripción Genética/genética , Dedos de Zinc/genéticaRESUMEN
Respiratory symptoms are frequent after bone marrow transplantation (BMT). Most studies focus on lesions of the lower respiratory tract. However, sinusitis is also common in this setting, especially after allogeneic BMT. The nasal respiratory epithelium is the first line of airway defense and is very similar to the bronchial epithelium, especially in terms of ciliary beat frequency and ultrastructural pattern of ciliated cells. We have prospectively studied the nasal respiratory epithelium of 20 marrow recipients (four autologous, 16 allogeneic) with or without sinusitis, by brushing and biopsy of the median turbinate between 2.5 and 148 months after transplant. Samples were studied for ciliary beat frequency, cytology, ultrastructural pattern and HLA-DR expression. We found that 17 of our 20 patients had abnormalities of their nasal epithelium, mainly consisting of either squamous metaplasia or heterogeneous axonemal defects of peripheral and central microtubules. No relationship between these findings and the presence of acute or chronic sinus infection, previous irradiation, graft-versus-host disease or immunosuppressive therapy could be demonstrated in this preliminary study. These abnormalities probably have multiple causes. Prospective studies are needed to determine the respective roles of treatments, infections and immune disorders associated with BMT in these abnormalities, and to know their natural evolution over time and their impact on the occurrence of upper or lower respiratory tract infections.
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Trasplante de Médula Ósea/patología , Cilios/ultraestructura , Mucosa Nasal/ultraestructura , Enfermedad Aguda , Adulto , Anemia Aplásica/terapia , Enfermedad Crónica , Cilios/fisiología , Epitelio/ultraestructura , Femenino , Enfermedad Injerto contra Huésped/complicaciones , Antígenos HLA-DR/análisis , Neoplasias Hematológicas/terapia , Humanos , Terapia de Inmunosupresión , Masculino , Metaplasia , Microtúbulos/ultraestructura , Persona de Mediana Edad , Estudios Prospectivos , Infecciones del Sistema Respiratorio/complicaciones , Infecciones del Sistema Respiratorio/patología , Factores de Riesgo , Sinusitis/complicaciones , Sinusitis/patología , Acondicionamiento Pretrasplante/efectos adversosRESUMEN
BACKGROUND/AIMS: Chronic hepatitis C virus infections are often associated with extra-hepatic immunological manifestations, including various autoimmune disorders. The aims of this study were: (i) to determine the prevalence of hepatitis C virus markers in patients with autoimmune thrombocytopenic purpura, and (ii) to determine whether a relationship could exist between autoimmune thrombocytopenic purpura and hepatitis C virus infections. METHODS: One hundred and thirty-nine patients with autoimmune thrombocytopenic purpura (45 men, 94 women, mean age 42 years, range 16-90) were studied. RESULTS: Anti-HCV antibodies were sought in their first and last available cryopreserved sera. In case of seropositivity, all their available cryopreserved sera were tested for anti-HCV antibodies and for HCV-RNA. Anti-HCV antibodies were detected in 14 of the 139 patients (10%). Four patients had transient anti-HCV seropositivity due to passive transfer of anti-HCV antibodies secondary to the infusion of intravenous immunoglobulin concentrates. Three patients seroconverted during follow up, due to intravenous drug use in one case, transfusion of non-HCV-screened blood units in one case, and infusion of intravenous immunoglobulins in one case. Seven patients had chronic hepatitis C discovered at the same time as autoimmune thrombocytopenic purpura. In two of them, hepatitis C virus transmission was the consequence of autoimmune thrombocytopenic purpura treatment but, in five cases, hepatitis C virus infection predated autoimmune thrombocytopenic purpura, so that the role of hepatitis C virus in autoimmune thrombocytopenic purpura could be suggested. CONCLUSIONS: Whereas hepatitis C virus does not appear to be the main etiological agent of autoimmune thrombocytopenic purpura can be envisaged. On the other hand, treatment of autoimmune thrombocytopenic purpura or autoimmune thrombocytopenic purpura-related symptoms by blood product infusion can be complicated by hepatitis C virus transmission.
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Hepatitis C/complicaciones , Púrpura Trombocitopénica Idiopática/etiología , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Hepacivirus/genética , Anticuerpos contra la Hepatitis C/sangre , Humanos , Masculino , Persona de Mediana Edad , ARN Viral/análisisRESUMEN
The Central African Republic is located in tropical Africa, where both the human immunodeficiency virus (HIV) and hepatitis B virus (HBV) are highly endemic. The exact prevalence of hepatitis C virus (HCV) and hepatitis E virus (HEV) markers in this country is unknown. The aim of the study was to determine, according to HIV and HBV serostatus, the prevalence of these markers in young sexually active adults in the Central African Republic. One hundred and fifty-seven consecutive patients attending the National Centre for Sexually Transmitted Diseases in Bangui were included. The following serological markers were examined: (i) anti-HIV1 and anti-HIV2 antibodies; (ii) markers of HBV infection; (iii) anti-HCV antibodies; (iv) anti-HEV antibodies. Anti-HIV1 antibodies were found in 31 of the 157 patients (20%). The prevalence of anti-HBc antibodies, reflecting exposure to HBV, was 140/157 (89%) and 45 had detectable anti-HBs antibodies. Twenty-two patients (14%) were chronic carriers of hepatitis B surface antigen (HBsAg), but only one was HBe antigen-positive. Anti-HCV antibodies were found in 8 persons (5%) and anti-HEV antibodies in 38 (24%). No difference was found in the prevalence of these markers according to the presence or absence of anti-HIV antibodies. This study confirms the high rate of HIV infection, HBV exposure and chronic carriage of HBsAg in sexually active young adults in the Central African Republic. A high prevalence of HCV markers was found in this population, similar to that reported in neighbouring countries, together with a high rate of HEV markers, suggesting that HEV is endemic in this region.
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Seroprevalencia de VIH , Anticuerpos Antihepatitis/sangre , Hepatitis B/epidemiología , Hepatitis C/epidemiología , Hepatitis E/epidemiología , Conducta Sexual , Adolescente , Adulto , República Centroafricana/epidemiología , Femenino , Infecciones por VIH/epidemiología , Infecciones por VIH/inmunología , VIH-1/inmunología , VIH-2/inmunología , Hepatitis B/inmunología , Anticuerpos contra la Hepatitis B/sangre , Hepatitis C/inmunología , Anticuerpos contra la Hepatitis C/sangre , Hepatitis E/inmunología , Humanos , Masculino , Persona de Mediana Edad , Prevalencia , Estudios SeroepidemiológicosRESUMEN
The aim of the present study was to examine whether there is an abnormal expression of certain endogenous retroviruses in MS patients. For this purpose samples of peripheral blood mononuclear cells were obtained from 22 MS patients, a corresponding number of age and sex-matched healthy donors and five patients with other diseases affecting the central nervous system. In addition, brain specimens of macroscopic normal white and gray matter from four MS patients and a similar number of controls were included in the study. Using an enzymatic amplification technique, we found expression of the endogenous retroviral sequences, HRES-1, HERV-K10 and ERV3 in most samples of peripheral blood mononuclear cells from MS patients and controls without obvious differences between these two groups. In contrast, composite transcripts of ERV3 and a zinc finger sequence were more frequently detected in healthy donors than in MS patients. At present, the possible significance of this is uncertain. The retroviral element 4-1 was not transcribed or only transcribed at a very low level in peripheral blood cells of controls and MS patients. Transcripts of various endogenous retroviruses were also detected in the brain samples, but a different pattern was not apparent in the MS group as compared with controls. Aspects concerning a possible association between endogenous retroviruses and autoimmunity are considered.
Asunto(s)
Encéfalo/virología , Leucocitos Mononucleares/virología , Esclerosis Múltiple/sangre , Esclerosis Múltiple/virología , Retroviridae/aislamiento & purificación , Adulto , Anciano , Cartilla de ADN , ADN Complementario/análisis , Desoxirribonucleasas de Localización Especificada Tipo II , Femenino , Amplificación de Genes/fisiología , Humanos , Recién Nacido , Masculino , Persona de Mediana Edad , Esclerosis Múltiple/genética , Reacción en Cadena de la Polimerasa , ARN Viral/análisis , Retroviridae/genética , Transcripción Genética/genética , Dedos de Zinc/genéticaRESUMEN
Recently, our group has shown that a 3-month course of intravenous immunoglobulin (2 g/kg/monthly) followed by 6 months of intramuscular immunoglobulins (IMIG, 16.5%, 0.35 ml/kg every 15 days) was able to slow or to stop the decline in the glomerular filtration rate, to reduce proteinuria, hematuria, leukocyturia and the histological index of activity on renal biopsy in patients with severe forms of IgA nephropathy (IGAN) and Henoch-Schönlein purpura (HSP). The aim of this open prospective trial was to evaluate the efficacy and safety of low-dose immunoglobulin therapy in moderate IGAN and HSP with permanent proteinuria. Fourteen patients with moderate IGAN [idiopathic IGAN: n = 11; chronic idiopathic HSP: n = 3] and permanent albuminuria were treated with polyvalent IMIG (16.5%) for 9 months (0.35 ml/kg once a week for 1 month, followed by 0.35 ml/kg every 15 days for a further 8 months). Eligibility criteria in the study were Lee histological stage I, II or III, albuminuria between 300 and 2,000 mg/day and a glomerular filtration rate > 70 ml/min/1.73 m2. IMIG were well tolerated and only 1 patient withdrew from the trial. No viral, renal or immunological side effects were observed. IMIG induced a significant decrease in albuminuria as well as in the histological activity index in the 11 cases in which a follow-up biopsy was performed. There was also a decrease in serum IgA, serum beta 2-microglobulin and IgA immune complex levels, and an increase in serum IgG1 levels. Twelve of the 13 evaluable patients improved during treatment.