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Cell Tissue Res ; 358(1): 257-69, 2014 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-24988912

RESUMEN

This study presents a comprehensive view of the histological and functional status of the prostate of adult rat offspring of mothers subjected to gestational diabetes induced by alloxan. The ventral prostate of male adult offspring of diabetic (DP) or normal (CP) mothers was evaluated for collagen fibres, cell death, fibroblasts, smooth muscle cells, cell proliferation, matrix metalloproteinases (MMPs), androgen receptors (AR), transforming growth factor ß1 (TGFß-1), catalase and total antioxidant activity. The prostates of DP animals were lower in weight than those of the CP group. The DP group also exhibited hyperglycaemia and hypotestosteronemia, higher cell proliferation and AR expression, a reduction in α-actin (possibly interfering with the reproductive function of the prostate), and enhanced activity of MMP-2, although the absolute content of MMP-2 was lower in this group. These findings were associated with increased TGFß-1 and decreased collagen distribution. The prostates of DP rats additionally exhibited reductions in catalase and total antioxidant activity. Thus, rats developing in a diabetic intrauterine environment have glycaemic and hormonal changes that impact on the structure and physiology of the prostate in adulthood. The increased AR expression possibly leads to elevated cell proliferation. Stromal remodelling was characterized by enhanced activity of MMP-2 and collagen degradation, even with increased TGFß-1 activation. These changes associated with increased oxidative stress might interfere with tissue architecture and glandular homeostasis.


Asunto(s)
Diabetes Mellitus Experimental , Diabetes Gestacional , Metaloproteinasa 2 de la Matriz/biosíntesis , Embarazo en Diabéticas , Efectos Tardíos de la Exposición Prenatal/enzimología , Próstata/enzimología , Animales , Colágeno/metabolismo , Femenino , Regulación de la Expresión Génica , Hiperglucemia/enzimología , Hiperglucemia/etiología , Hiperglucemia/patología , Masculino , Estrés Oxidativo , Embarazo , Efectos Tardíos de la Exposición Prenatal/patología , Próstata/patología , Ratas , Ratas Wistar , Receptores Androgénicos/biosíntesis , Factor de Crecimiento Transformador beta1/biosíntesis
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