RESUMEN

Regulation of the concentration of Ca2+ in the apoplast of plants is essential in order to allow Ca(2+)-dependent processes, such as Ca(2+)-mediated signal transduction in stomatal guard cells, to function correctly. This is particularly important for plants growing with high levels of Ca2+ in the rhizosphere. Recently, we have shown that in two calcicoles, Leontodon hispidus L. and Centaurea scabiosa L., trichomes play a key role in this regulatory process. Ozone is known to have a marked effect on plant Ca2+ homeostasis. Therefore, we have examined the effect of this pollutant on the regulation by trichomes of apoplastic Ca2+ in the calcicole L. hispidus. Treatment with 100 nl l-1 ozone resulted in a reduction in stomatal conductance of approximately 25% in plants grown with 15 mM Ca2+ in the rhizosphere. Analysis of total Ca2+ levels revealed that these changes in stomatal behaviour reflect a decrease in the ability of trichomes to sequester Ca2+. The amount of Ca2+ present in the trichome tip cell was reduced by approximately 38%. This was accompanied by an increase in the levels of Ca2+ in the guard cells and other tissues of the leaf. These data suggest that ozone has a detrimental effect on the ability of trichomes to regulate the concentration of apoplastic Ca2+ in L. hispidus, resulting in altered stomatal behaviour, and hence gaseous exchange, possibly due to the disruption of guard-cell Ca(2+)-mediated signal transduction. This has important implications for the growth and survival of plants growing in Ca(2+)-rich soils.

Asunto(s)

Asteraceae/metabolismo , Cloruro de Calcio/metabolismo , Ozono/toxicidad , Estructuras de las Plantas/metabolismo , Adaptación Fisiológica/fisiología , Contaminantes Atmosféricos/toxicidad , Asteraceae/efectos de los fármacos , Cloruro de Calcio/administración & dosificación , Extensiones de la Superficie Celular/metabolismo , Pared Celular/metabolismo , Ozono/administración & dosificación , Estructuras de las Plantas/efectos de los fármacos , Transducción de Señal , Suelo/análisis

RESUMEN

Experiments are described which attempted to clarify the reasons for differences in stomatal opening on adaxial and abaxial epidemics. Previous studies had suggested that endogenous auxin levels might be responsible. It was found that adaxial stomata were more sensitive than abaxial stomata to externally supplied abscisic acid (ABA). It is suggested that differing endogenous concentrations of calcium ions in the upper and lower epidermes may contribute to the observed differences in sensitivity to ABA. It is further suggested that studies of stomata on isolated epidermis may represent a useful model system for examining the role of cell or tissue sensitivity in determining the responses to ABA and other hormones. Data are presented which lend support to the suggestion that calcium ions play an important part. Treatment with lanthanum ions, which block calcium channels in cell membranes, had little effect on abaxial stomata, but caused the apertures of adaxial stomata to increase considerably. The chelating agent EGTA eliminated the differential behaviour of adaxial and abaxial stomata, which clearly suggests that calcium ions have a dominant role. Experiments in which EGTA and fusicoccin were presented separately and in combination indicated that their effects were independent and additive. It is suggested that they operate at different locations, or on different processes in the guard cells.