RESUMEN
Excessive alcohol consumption causes metabolic changes and pathologic alterations in testes and accessory sex organ in different animal species. The aim of the present study was to evaluate the macroscopic, histologic and ultrastructural alterations provoked by chronic ingestion of different ethanol concentrations over increasing periods of time on the secretory epithelium of the seminal vesicle of C57/BL/6J mice in using stereological methods. Sixty male adult mice were divided into three experimental groups: Control, Alcoholic 25% and Alcoholic 35%, respectively, receiving tap water and tap water containing ethanol diluted to 25 and 35 degrees Gay Lussac. All mice were fed with the same solid diet. After 150 and 250 days of treatment the animals were sacrificed and the seminal vesicles were collected and processed for light and transmission electron microscopy. The cellular, cytoplasmic and nuclear volumes and the area density of autophagic and secretory vacuoles were measured. The histologic alterations observed in the alcoholic mice consisted of a reduction in epithelial size and cell volume, with maintenance of the same nuclear and cytoplasmic ratio as verified in the control groups. The ultrastructural alterations were: increased density of dense body area, decreased density of secretory granule area, and dilated rough endoplasmic reticulum and Golgi cisternae. We conclude that chronic ethanol ingestion causes depleting morphologic alterations in the epithelial cells of the seminal vesicle and negatively affects the secretory process of this gland.
Asunto(s)
Alcoholismo/patología , Células Epiteliales/efectos de los fármacos , Vesículas Seminales/efectos de los fármacos , Alcoholismo/fisiopatología , Animales , Peso Corporal/efectos de los fármacos , Depresores del Sistema Nervioso Central/toxicidad , Células Epiteliales/patología , Células Epiteliales/ultraestructura , Etanol/toxicidad , Masculino , Ratones , Ratones Endogámicos C57BL , Microscopía Electrónica , Vesículas Seminales/patología , Vesículas Seminales/ultraestructura , Vacuolas/patología , Vacuolas/ultraestructuraRESUMEN
As nadadeiras dos teleósteos säo constituídas por unidades esqueléticas segmentares denominadas lepidotriquiais ou raios. Essas nadadeiras estäo envolvidas em processos regenerativos: assim, o conhecimento de sua estruturaçäo normal é de fundamental importância tanto para a compreensäo do processo de crescimento como de sua regeneraçäo. Com o objetivo de detectar substâncias lipídicas, as nadadeiras caudais foram fixadas pela formalina contendo bicloreto de cálcio a 3 por cento por 24 horas, à temperatura ambiente. Cortes com 10um de espessura, obtidos em micrótomo de congelaçäo foram submetidos às seguintes reaçöes histoquímicas específicas para lipídios: 1) Permanganato de Potássio Alcalino - Azul de Toluidina, para detecçäo de vitamina D; 2) Acido Perfórmico-Azul de Toluidina, para detecçäo de vitamina E e 3) Acido Peracético-Azul de Toluidina, para detecçäo de colesterol. Os controles para os testes histoquímicos 1, 2 e 3 foram feitos omitindo-se o agente oxidante. Reaçöes positivas obtidas para as reaçöes 1, 2 e 3 demonstraram a presença de vitamina D, E e colesterol nos raios das nadadeiras caudais da espécie Tilapia rendalli