RESUMEN
The development of preventive programs targeting specific groups of consumers of medical social services having age-related health and lifestyle characteristics is one of the most prospective methods to improve quality of medical care of the elderly and senile patients. The previously developed by Russian and foreign scientists programs of personalized prevention of age-related diseases make it possible to organize target groups of patients seeking medical services in both state and private health care organizations to be screened for conditions of medical and social significance from the point of view of preventive medicine. This permits to achieve a real integration of various components of preventive care that improves health of elderly and senile patients in terms of morphofunctional indices of patient's physical status, quality of life, degree of independence, and, as a result, quality and scope of implementation of preventive programs for elderly population. Therefore, the development of the basics of personalized programs preventing age-related diseases in elderly population is actual.
Asunto(s)
Atención a la Salud , Calidad de Vida , Anciano , Humanos , Servicios Preventivos de Salud , Estudios Prospectivos , Federación de RusiaRESUMEN
Continuous cell cultures are sensitive test systems used not only for the determination of the toxicity of diphtheria exotoxin, but also for revealing the toxic properties of adsorbed DPT vaccine. Morphological changes induced by the action of diphtheria exotoxin and adsorbed DPT vaccine in the cultures of L929, HeLa, FL, L132 and monkey tonsil cells are similar in character. The effect produced by the diphtheria exotoxin and adsorbed DPT vaccine on cell cultures may be characterized as toxic, subtoxic and minimal. Nevertheless, even in the cultures treated with minimal concentrations the pathological state of the cells can be detected in the next 2 or 3 serial subcultures.
Asunto(s)
Toxoide Diftérico/toxicidad , Vacuna contra la Tos Ferina/toxicidad , Toxoide Tetánico/toxicidad , Amnios , Animales , Línea Celular , Haplorrinos , Células HeLa , Humanos , Células L , Métodos , Ratones , Tonsila PalatinaRESUMEN
A model on a HEp-2 cell culture was elaborated, permitting the study of the ability of microbes to be adsorbed an to proliferate on the surface of cells and of the mechanism of their protective effect. The ability of E. coli strains to be adsorbed and to proliferate on the surface of a cell culture was found to differ. It has been demonstrated that the protection of the cell culture from subsequent infection with virulent Shigella can be explained not only by the antagonistic activity of E. coli strains, but also by their ability to be adsorbed and to proliferate on the surface of cells. A similar mechanism of protective effect is supposed in preparations of the Colibacterin type.
Asunto(s)
Disentería Bacilar/prevención & control , Escherichia coli , Células Cultivadas , Colicinas/biosíntesis , Escherichia coli/crecimiento & desarrollo , Absorción IntestinalRESUMEN
The duration of detection of the specific antigen in the cells of the central nervous system (CNS) of mice extraneurally infected with 450, 560 and 4380 LD50 of rabies virus, the Vnukovo-32 strain was studied. The antigen in the CNS cells was detected by the fluorescent antibody technique from the 11th until the 52nd day postinoculation, at 16-34 days being found in 50 to 100% of the experimental animals. In parallel, virus-neutralizing antibody was determined in the blood serum of the infected mice, and their brains were examined for the presence of rabies virus by bioassays. In most cases, serological results correlated with the antigen detection in the brain tissue. At 18, 29 and 34 days rabies virus was isolated from clinically normal mice inoculated subcutaneously, which by all the tests used did not differ from the other experimental animals. The results of the experiments attest to the possibility of rabies virus, the Vnukovo-32 strain, persistence in mice.