RESUMEN
It is well known that the principle of reciprocity is valid for light traveling even through scattering or absorptive media. This principle has been used to establish an equivalence between conventional widefield microscopes and scanning microscopes. We make use of this principle to introduce a scanning version of oblique back-illumination microscopy, or sOBM. This technique provides sub-surface phase-gradient and amplitude images from unlabeled tissue, in an epi-detection geometry. That is, it may be applied to arbitrarily thick tissue. sOBM may be implemented as a simple, cost-effective add-on with any scanning microscope, requiring only the availability of an extra input channel in the microscope electronics. We demonstrate here its implementation in combination with two-photon excited fluorescence (TPEF) microscopy and with coherent anti-Stokes Raman scattering (CARS) microscopy, applied to brain or spinal cord tissue imaging. In both cases, sOBM provides information on tissue morphology complementary to TPEF or CARS contrast. This information is obtained simultaneously and is automatically co-registered. Finally, we show that sOBM can be operated at video rate.
RESUMEN
We measured Ca2+ concentration, [Ca2+], transients in mitral cell distal apical dendritic tufts produced by physiological odour stimulation of the olfactory epithelium and electrical stimulation of the olfactory nerve (ON) using two-photon scanning and conventional wide-field microscopy of Ca2+-Green-1 dextran in an in vitro frog nose-brain preparation. Weak or strong ON shock-evoked fluorescence transients always had short latency with an onset 0-10 ms after the onset of the bulb local field potential, rapidly increasing to a peak of up to 25% fractional fluorescence change (DeltaF/F) in 10-30 ms, were blocked by 10 microM CNQX, decaying with a time constant of about 1 s. With stronger ON shocks that activated many receptor axons, an additional, delayed, sustained AP5-sensitive component (peak at approximately 0.5 s, up to 40% DeltaF/F maximum) could usually be produced. Odour-evoked [Ca2+] transients sometimes displayed a rapid onset phase that peaked within 50 ms but always had a sustained phase that peaked 0.5-1.5 s after onset, regardless of the strength of the odour or the amplitude of the response. These were considerably larger (up to 150% DeltaF/F) than those evoked by ON shock. Odour-evoked [Ca2+] transients were also distinguished from ON shock-evoked transients by tufts in different glomeruli responding with different delays (time to onset differed by up to 1.5 s between different tufts for the same odour). Odour-evoked [Ca2+] transients were increased by AMPA-kainate receptor blockade, but substantially blocked by AP5. Electrical stimulation of the lateral olfactory tract (5-6 stimuli at 10 Hz) that evoked granule cell feedback inhibition, blocked 60-100% of the odour-evoked [Ca2+] transient in tufts when delivered within about 0.5 s of the odour. LOT-mediated inhibition was blocked by 10 microM bicuculline.
Asunto(s)
Señalización del Calcio/fisiología , Dendritas/metabolismo , Odorantes , Bulbo Olfatorio/metabolismo , Rana pipiens/metabolismo , Olfato/fisiología , Animales , Señalización del Calcio/efectos de los fármacos , Dendritas/efectos de los fármacos , Dendritas/ultraestructura , Regulación hacia Abajo/efectos de los fármacos , Regulación hacia Abajo/fisiología , Estimulación Eléctrica , Retroalimentación/efectos de los fármacos , Retroalimentación/fisiología , Colorantes Fluorescentes/farmacocinética , Potenciales de la Membrana/efectos de los fármacos , Potenciales de la Membrana/fisiología , Modelos Animales , Inhibición Neural/efectos de los fármacos , Inhibición Neural/fisiología , Bulbo Olfatorio/citología , Bulbo Olfatorio/efectos de los fármacos , Vías Olfatorias/efectos de los fármacos , Vías Olfatorias/fisiología , Neuronas Receptoras Olfatorias/citología , Neuronas Receptoras Olfatorias/metabolismo , Rana pipiens/anatomía & histología , Tiempo de Reacción/efectos de los fármacos , Tiempo de Reacción/fisiología , Receptores AMPA/efectos de los fármacos , Receptores AMPA/metabolismo , Receptores de N-Metil-D-Aspartato/efectos de los fármacos , Receptores de N-Metil-D-Aspartato/metabolismo , Olfato/efectos de los fármacos , Transmisión Sináptica/efectos de los fármacos , Transmisión Sináptica/fisiología , Factores de TiempoRESUMEN
Dextran-conjugated Ca(2+) indicators were injected into the accessory olfactory bulb of frogs in vivo to selectively fill presynaptic terminals of mitral cells at their termination in the ipsilateral amygdala. After one to three days of uptake and transport, the forebrain hemisphere anterior to the tectum was removed and maintained in vitro for simultaneous electrophysiological and optical measurements. Ca(2+) influx into these terminals was compared to synaptic transmission between mitral cells and amygdala neurons under conditions of reduced Ca(2+) influx resulting from reduced extracellular [Ca(2+)], blockade of N- and P/Q-type channels, and application of the cholinergic agonist carbachol. Reducing extracellular [Ca(2+)] had a non-linear effect on release; release was proportional to Ca(2+) influx raised to the power of approximately 3.6, as observed at numerous other synapses. The N-type Ca(2+) channel blocker, omega-conotoxin-GVIA (1 microM), blocked 77% of Ca(2+) influx and 88% of the postsynaptic field potential. The P/Q-type Ca(2+) channel blocker, omega-agatoxin-IVA (200 nM), blocked 19% of Ca(2+) influx and 25% of the postsynaptic field, while the two toxins combined to block 92% of Ca(2+) influx and 97% of the postsynaptic field. The relationship between toxin blockade of Ca(2+) influx and synaptic transmission was therefore only slightly non-linear; release was proportional to Ca(2+) influx raised to the power approximately 1.4. Carbachol (100 microM) acting via muscarinic receptors had no effect on the afferent volley, but rapidly and reversibly reduced Ca(2+) influx through both N- and P/Q-type channels by 51% and postsynaptic responses by 78%, i.e. release was proportional to Ca(2+) raised to the power approximately 2.5. The weak dependence of release on changes in Ca(2+) when channel toxins block channels suggests little overlap between Ca(2+) microdomains from channels supporting release or substantial segregation of channel subtypes between terminals. The proportionately greater reduction of transmission by muscarinic receptors compared to Ca(2+) channel toxins suggests that they directly affect the release machinery in addition to reducing Ca(2+) influx.
Asunto(s)
Amígdala del Cerebelo/metabolismo , Canales de Calcio/metabolismo , Señalización del Calcio/fisiología , Neurotransmisores/metabolismo , Terminales Presinápticos/metabolismo , Rana pipiens/metabolismo , Transmisión Sináptica/fisiología , Amígdala del Cerebelo/citología , Amígdala del Cerebelo/efectos de los fármacos , Animales , Bloqueadores de los Canales de Calcio/farmacología , Canales de Calcio/efectos de los fármacos , Señalización del Calcio/efectos de los fármacos , Colorantes Fluorescentes/farmacocinética , Indicadores y Reactivos/farmacocinética , Masculino , Potenciales de la Membrana/efectos de los fármacos , Potenciales de la Membrana/fisiología , Modelos Neurológicos , Agonistas Muscarínicos/farmacología , Antagonistas Muscarínicos/farmacología , Vías Nerviosas/citología , Vías Nerviosas/efectos de los fármacos , Vías Nerviosas/metabolismo , Bulbo Olfatorio/citología , Bulbo Olfatorio/efectos de los fármacos , Bulbo Olfatorio/metabolismo , Terminales Presinápticos/efectos de los fármacos , Terminales Presinápticos/ultraestructura , Rana pipiens/anatomía & histología , Receptores Muscarínicos/efectos de los fármacos , Receptores Muscarínicos/metabolismo , Transmisión Sináptica/efectos de los fármacosRESUMEN
Prevalence estimates for psychiatric disturbance in mothers and their 2 1/2 year old children and the variations in prevalence associated with marriage quality, social class and the child's developmental level are presented. It was found that both the mother's psychiatric disturbance, and more specifically, depression were associated most strongly with child disturbance, poor marriage quality and low child developmental level. For toddler disturbance, the strong associations were with type and severity of disturbance in mother, social class, marriage quality and low developmental quotient.
Asunto(s)
Trastornos de la Conducta Infantil/psicología , Trastornos Mentales/psicología , Relaciones Madre-Hijo , Adulto , Trastornos de la Conducta Infantil/diagnóstico , Trastornos de la Conducta Infantil/epidemiología , Preescolar , Trastornos del Conocimiento/diagnóstico , Femenino , Humanos , Masculino , Matrimonio/psicología , Bienestar Materno , Trastornos Mentales/diagnóstico , Trastornos Mentales/epidemiología , Prevalencia , Índice de Severidad de la Enfermedad , Clase Social , Encuestas y CuestionariosRESUMEN
A dynamic model for a swimming scallop was developed which integrates the mechanical properties of the hinge ligaments, valve inertia, the external fluid-flow reaction, the fluid pressure in the mantle cavity and the muscle contraction. Kinematic data were recorded for a swimming Placopecten magellanicus from high-speed film analysis. Dynamic loading experiments were performed to provide the required mechanical properties of the hinge for the same species. The swimming dynamics and energetics based on data from a 0.065 m long Placopecten magellanicus at 10 °C were analyzed. The main conclusions are as follows. 1. The mean period of a clapping cycle during swimming is about 0.28 s, which can be roughly divided into three equal intervals: closing, gliding and opening. The maximum angular velocity and acceleration of the valve movements are about 182 degrees s-1 and 1370 degrees s-2, respectively. 2. The hysteresis loop of the hinge was found to be close to an ellipse. This may be represented as a simple Voigt body consisting of a spring and dashpot in parallel, with a rotational stiffness of 0.0497 N m and viscosity coefficient of 0.00109 kg m2 s-1 for the 0.065 m long Placopecten magellanicus. 3. The external fluid reaction has three components, of which the added mass is about 10 times higher than the mass of a single valve, and the flow-induced pseudo-viscosity compensates for nearly half of the hinge viscosity for the 0.065 m long Placopecten magellanicus. 4. The locomotor system powered by the muscle can be divided into two subsystems: a pressure pump for jet production and a shell-hinge/outer-fluid oscillator which drives the pumping cycle. The dynamics of the oscillator is determined predominantly by the interaction of the external fluid reaction and the hinge properties, and its resonant frequency was found to be close to the swimming frequencies. 5. The momentum and energy required to run the oscillator are negligibly small (about 1 % for the 0.065 m long Placopecten magellanicus) compared with that for the jet. Almost all the mechanical energy from muscle contraction is used to perform hydrodynamic work for jet production. Thus, the Froude efficiency of propulsion in scallops is nearly the same as the entire mechanical efficiency of the locomotor system. This could be a fundamental advantage of jet propulsion, at least for a scallop. 6. The estimated maximum muscle stress is about 1.06x10(5) N m-2, the cyclic work is 0.065 J and power output is 1.3 W. Using an estimate of the mass of an adductor muscle, the work done by the muscle per unit mass is 9.0 J kg-1 and the peak power per unit mass is 185 W kg-1. 7. The time course of the force generation of the contracting adductor muscle is basically the same as that of the hydrodynamic propulsive force.
RESUMEN
Light and electron microscopy and in vitro inflation experiments were conducted on the aortae of three different invertebrate species: the lobster Homarus americanus, the horseshoe crab Limulus polyphemus and the whelk Busycon contrarium. Inflation experiments were also performed on the aortae of two species of primitive vertebrates, the sea lamprey Petromyzon marinus and the Atlantic hagfish Myxine glutinosa. The inflation experiments demonstrated similar overall biomechanical properties in each case, despite the existence of differences in tissue structure. The vessels were compliant at low strains, but demonstrated nonlinear elasticity, increasing in stiffness as strains increased; this property could act as protection against artery wall rupture. The vessels of the lamprey, hagfish and lobster are capable of acting as fairly efficient elastic reservoirs and of smoothing blood flow during circulation as they had low hysteresis values (13-18%). The aortae of the horseshoe crab and whelk, if performing this function, have much higher energy losses, up to more than 30% per cycle. The microscopy studies of the aortae of the lobster, horseshoe crab and whelk revealed tissue structures which differ widely from each other as well as from the structures of the lamprey and hagfish. None of these arteries contained elastin, but all contained fibrillar material which differed in appearance, size and arrangement between species. These materials were conjectured to contribute to the elastic properties of the tissue.
Asunto(s)
Aorta/ultraestructura , Peces/anatomía & histología , Cangrejos Herradura/anatomía & histología , Moluscos/anatomía & histología , Nephropidae/anatomía & histología , Animales , Aorta/fisiología , Fenómenos Biomecánicos , Circulación Sanguínea , Tejido Elástico , Elasticidad , Peces/fisiología , Cangrejos Herradura/fisiología , Moluscos/fisiología , Músculo Liso Vascular/fisiología , Músculo Liso Vascular/ultraestructura , Nephropidae/fisiologíaRESUMEN
Extensive characterization of the vitronectin receptor (VNR), a member of the integrin group of cell adhesion molecules, which is abundantly expressed in osteoclasts, has revealed a role for this receptor in osteoclast adhesion as well as bone resorption. Earlier evidence from our laboratory suggests that VNR is also capable of transducing intracellular signals following receptor ligand interaction, although this function is poorly understood. Thus, addition of peptides containing the minimal tripeptide Arg-Gly-Asp (RGD) integrin recognition sequence elicits transient increases in intracellular free calcium ions, with maximal responses seen with a bone sialoprotein peptide, BSP-IIA. In the present study we have attempted to determine some of the structural requirements for calcium signaling in osteoclasts using derivatives of the peptide PRGDN/T sequence found in bone sialoprotein. While some peptides, such as the parent sequence PRGDN, can induce both signaling and retractile events, it was found that minor structural modifications yielded peptides such as PRADN which elicited a transient increase in intracellular free calcium ions without promoting a reduction in osteoclast spread area (retraction). Conversely, certain other modifications resulted in peptides, such as PrGDN and benzoyl-RGDN, which effect osteoclast retraction, while having minimal Ca2+ signaling capabilities. Osteoclast adhesion, and hence retraction, are known to be RGD-dependent and integrin-dependent events. However, intracellular Ca2+ signaling is RGD-independent and, based on lack of inhibition by an anti-beta 3 integrin antibody F11 and echistatin, very likely integrin-independent. These data suggest that signaling is not always via VNR and as yet unknown receptors on the osteoclast membrane play a role in osteoclast signaling and hence function.
Asunto(s)
Calcio/fisiología , Integrinas/fisiología , Oligopéptidos/fisiología , Osteoclastos/citología , Receptores de Citoadhesina/fisiología , Transducción de Señal , Secuencia de Aminoácidos , Animales , Adhesión Celular , Sialoproteína de Unión a Integrina , Datos de Secuencia Molecular , Osteoclastos/metabolismo , Ratas , Ratas Sprague-Dawley , Receptores de Vitronectina , Sialoglicoproteínas/metabolismoRESUMEN
In rat anterior pituitary tumour cells (GH3/B6) thyrotropin-releasing hormone (TRH) elicits a biphasic response. First, a release of intracellularly stored Ca2+ induces a hyperpolarization of the cell. Second, a depolarization thought to be induced by a reduction of the inward-rectifying K+ current (KIR) causes an increase in action potential frequency and a plateau-like increase in [Ca2+]i. It has been proposed that the two phases are induced by the actions of inositol 1,4,5-trisphosphate (InsP3) and protein kinase C (PKC), respectively, but we demonstrate here that PKC is not responsible for the second phase increase in [Ca2+]i and suggest that the pathways diverge at the level of receptor and G protein coupling. Both phases of the TRH response were insensitive to pertussis toxin, but cholera toxin (CTX) selectively affected the second phase. After CTX pretreatment cells had a high spontaneous spiking frequency and smaller KIR amplitude. In these cells TRH failed to increase the action potential frequency after the first phase hyperpolarization, elicited only a transient peak increase in [Ca2+]i with no plateau phase and could only slightly reduce KIR. These effects of CTX are not mediated by its ability to increase cAMP via activation of GS, as increased cAMP levels neither inhibit KIR nor prevent its reduction by TRH. In addition, inhibition of protein kinase A activation did not block the second phase increase in [Ca2+]i induced by TRH, suggesting that the CTX-sensitive G protein mediating the second phase of the TRH response is not GS.
Asunto(s)
Proteínas de Unión al GTP/fisiología , Hipófisis/fisiología , Hormona Liberadora de Tirotropina/farmacología , Potenciales de Acción/efectos de los fármacos , Animales , Células Cultivadas , Toxina del Cólera/farmacología , Proteínas Quinasas Dependientes de AMP Cíclico/antagonistas & inhibidores , Proteínas Quinasas Dependientes de AMP Cíclico/metabolismo , Activación Enzimática/efectos de los fármacos , Procesamiento de Imagen Asistido por Computador , Nistatina , Técnicas de Placa-Clamp , Toxina del Pertussis , Hipófisis/citología , Canales de Potasio/efectos de los fármacos , Canales de Potasio/metabolismo , Proteína Quinasa C/antagonistas & inhibidores , Proteína Quinasa C/metabolismo , Ratas , Factores de Virulencia de Bordetella/farmacologíaAsunto(s)
Calcio/metabolismo , Exocitosis , Integrinas/fisiología , Lisosomas/enzimología , Oligopéptidos/farmacología , Osteoclastos/metabolismo , Péptidos/farmacología , Secuencia de Aminoácidos , Animales , Núcleo Celular/efectos de los fármacos , Núcleo Celular/metabolismo , Exocitosis/efectos de los fármacos , Homeostasis , Péptidos y Proteínas de Señalización Intercelular , Líquido Intracelular/metabolismo , Datos de Secuencia Molecular , Osteoclastos/citología , Osteoclastos/efectos de los fármacos , Ratas , Venenos de Víboras/farmacologíaRESUMEN
Cell-matrix interactions have been shown to play an important role in regulating cell function and behaviour. In bone, where calcified matrix formation and resorption events are required to be in dynamic equilibrium, regulation of adhesive interactions between bone cells and their matrix is critical. The present study focuses on the osteoclast, the bone resorbing cell, as well as integrins, which are cell surface adhesion receptors that mediate osteoclast attachment to bone matrix. In osteoclasts, the most abundant integrin receptor is the vitronectin receptor (VNR, alpha v beta 3). The objective of the study was to investigate changes in intracellular calcium, a regulator of osteoclast function, following addition of peptides that bind integrins, in particular the alpha v beta 3 form of the vitronectin receptor (VNR), which is highly expressed in osteoclasts. The study demonstrated a unique spatial localisation of the calcium signal in response to cell membrane receptor occupancy by integrin ligands in rat osteoclasts. Addition of peptides with the Arg-Gly-Asp (RGD) sequence such as BSP-IIA, GRGDSP and GRGDS to rat osteoclasts evoked an immediate increase in free calcium ion concentration [Ca2+]i, localised to the nuclei and to the thin cytoplasmic skirt. These responses were inhibited by F11, a monoclonal antibody to the rat integrin beta 3 chain, as well as echistatin, a snake venom shown to colocalise with the alpha v chain in osteoclasts, suggesting that the calcium signal is mediated by the alpha v beta 3 form of VNR.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Calcio/metabolismo , Núcleo Celular/metabolismo , Matriz Extracelular/metabolismo , Osteoclastos/metabolismo , Péptidos , Receptores de Citoadhesina/fisiología , Animales , Anticuerpos Monoclonales/farmacología , Calcitonina/farmacología , Adhesión Celular , Comunicación Celular , Núcleo Celular/efectos de los fármacos , Células Cultivadas/efectos de los fármacos , Células Cultivadas/metabolismo , Péptidos y Proteínas de Señalización Intercelular , Oligopéptidos/farmacología , Osteoclastos/efectos de los fármacos , Ratas , Receptores de Vitronectina , Venenos de Víboras/farmacologíaRESUMEN
Serum samples from patients suffering from multiple sclerosis, other neurological diseases and normal controls were screened by "western blotting" for antibody directed against proteins of human brain vessels purified from a post mortem brain. A small number of sera contained autoantibodies against some of the proteins of the brain vessels, particularly in patients suffering from MS, epilepsy and migraine. The significance of these results is discussed.
Asunto(s)
Autoanticuerpos/análisis , Western Blotting , Encéfalo/irrigación sanguínea , Endotelio Vascular/inmunología , Esclerosis Múltiple/inmunología , Músculo Liso Vascular/inmunología , Especificidad de Anticuerpos/inmunología , Barrera Hematoencefálica/inmunología , Humanos , Inmunoglobulina G/análisis , Microscopía Electrónica de Rastreo , Enfermedades del Sistema Nervioso/inmunologíaRESUMEN
The relative contribution of magnocellular and parvocellular neurones to the patterning of milk ejections was studied by activation of various parts of the hypothalamo-neurohypophysial tract. During suckling of anaesthetised lactating rats, electrical stimulation of the neurohypophysis/neural stalk (15 Hz, 1.5 s on/1.5 s off, 10 min) evoked an increased frequency of milk ejections as detected by intramammary pressure recording. Bilateral stimulation of the paraventricular nuclei produced a similar facilitation in the post-stimulus period, but stimulation of the supraoptic nuclei (with either low current or current sufficient to evoke peripheral oxytocin release) had no effect on the occurrence of milk ejections. These data are consistent with the hypothesis that parvocellular but not magnocellular neurones participate in the regulation of milk ejection frequency.
Asunto(s)
Hipotálamo/fisiología , Eyección Láctea/fisiología , Neurohipófisis/fisiología , Animales , Estimulación Eléctrica , Femenino , Lactancia/efectos de los fármacos , Lactancia/fisiología , Eyección Láctea/efectos de los fármacos , Oxitocina/farmacología , Núcleo Hipotalámico Paraventricular/fisiología , Ratas , Ratas Endogámicas , Técnicas Estereotáxicas , Núcleo Supraóptico/fisiologíaAsunto(s)
Hipotálamo/efectos de los fármacos , Receptores Opioides/efectos de los fármacos , 3,4-Dicloro-N-metil-N-(2-(1-pirrolidinil)-ciclohexil)-bencenacetamida, (trans)-Isómero , Animales , Femenino , Fentanilo/farmacología , Hipotálamo/metabolismo , Lactancia/fisiología , Neuronas/efectos de los fármacos , Neuronas/fisiología , Oxitocina/metabolismo , Embarazo , Pirrolidinas/farmacología , Ratas , Receptores Opioides/fisiología , Receptores Opioides kappa , Receptores Opioides mu , Vasopresinas/metabolismoRESUMEN
A multicriterion screen made up of the General Health Questionnaire, the Behaviour Checklist and a health visitor questionnaire was given in a community project. This paper describes the usefulness of the screen and compares it with others which might be used in similar situations.
Asunto(s)
Trastornos de la Conducta Infantil/psicología , Desarrollo Infantil , Relaciones Madre-Hijo , Adulto , Terapia Conductista , Trastornos de la Conducta Infantil/terapia , Crianza del Niño , Preescolar , Femenino , Humanos , Masculino , Pruebas Psicológicas , PsicometríaRESUMEN
Dimensions of temperament and behaviour were measured in a group of infant school children with recurrent abdominal pain and in a control group. Children with recurrent abdominal pain were temperamentally more difficult than those without, and in particular, girls were found to have a more irregular temperamental style and boys to be more likely to withdraw in new situations. Temperamental differences were more persistent than the abdominal pain itself. There was little difference behaviourally between the groups. It is hypothesized that abdominal pain represents an interaction between a vulnerable temperamental style and environmental stresses.
Asunto(s)
Abdomen , Dolor/psicología , Personalidad , Temperamento , Niño , Trastornos de la Conducta Infantil/complicaciones , Femenino , Estudios de Seguimiento , Humanos , Masculino , Dolor/complicaciones , Recurrencia , Instituciones Académicas , Ajuste Social , Estrés Psicológico/complicacionesRESUMEN
This paper summarizes the preliminary thinking, the hypotheses and design and some preliminary results of a large community-based intervention project which is currently underway in the North East of England.