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1.
Redox Rep ; 5(2-3): 133-6, 2000.
Artículo en Inglés | MEDLINE | ID: mdl-10939294

RESUMEN

UVB-irradiated HeLa cells undergoing apoptosis have increased cell surface protease (CSP) activity compared to viable or necrotic cells. In order to elucidate whether caspase 3 plays a role in the activation of CSP in cells undergoing apoptosis, HeLa cell cultures were pre-treated with the caspase inhibitor, DEVD, prior to being exposed to 500 Jm(-2) UVB. DEVD significantly inhibited caspase 3 activity in cells undergoing apoptosis, but did not affect the activation of CSP in these cells. The findings suggest that the activation of CSP in apoptotic cells is unrelated to caspase 3 activity.


Asunto(s)
Apoptosis/fisiología , Membrana Celular/enzimología , Endopeptidasas/metabolismo , Rayos Ultravioleta , Apoptosis/efectos de los fármacos , Supervivencia Celular/efectos de la radiación , Inhibidores de Cisteína Proteinasa/farmacología , Endopeptidasas/efectos de la radiación , Células HeLa , Humanos , Leucina/análogos & derivados , Leucina/farmacología , Necrosis
2.
J Cell Biochem ; 76(4): 625-38, 2000 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-10653982

RESUMEN

Release from the cell surface of a variety of growth factors, cytokines, and proteases follows exposure to genetically stressful agents capable of inducing apoptosis and necrosis. Increased ectoprotease activity is responsible for their release. We show that increased activity of several metalloproteases on the HeLa cell surface occurs after stresses due to UVC, actinomycin D, cycloheximide, and cisplatinum, which induce the release of transforming growth factor-alpha (TGFalpha) and other bioactive molecules. The ectoprotease activities increase preferentially on apoptotic cells, while little change occurs in viable cells. Gross decreases, except for the putative TGFalphaase activity, accompany necrosis. These changes may contribute to tissue repair and the absence of an inflammatory reaction to apoptotic cell death. They appear to be due to preferential enzyme activation or to retention by cells undergoing significant categorical decreases in protein content.


Asunto(s)
Apoptosis/efectos de los fármacos , Membrana Celular/enzimología , Metaloendopeptidasas/metabolismo , Aminopeptidasas/metabolismo , Cisplatino/farmacología , Cicloheximida/farmacología , Fragmentación del ADN , Dactinomicina/farmacología , Citometría de Flujo , Células HeLa , Humanos , Leucina/análogos & derivados , Leucina/farmacología , Mutágenos/farmacología , Péptidos/metabolismo , Inhibidores de Proteasas/farmacología , Factor de Crecimiento Transformador alfa/metabolismo , Rayos Ultravioleta
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