RESUMEN

Asymptomatic infection with simian immunodeficiency virus (SIV) has been demonstrated in African Sykes' monkeys (Cercopithecus mitis albogularis), and virus isolation confirmed infection with a novel SIV from Sykes' monkeys (SIVsyk). Macaques inoculated with SIVsyk became persistently infected but remained clinically healthy. We utilized polymerase chain reaction amplification to generate a full-length, infectious molecular clone of SIVsyk. The genome organization of SIVsyk is similar to that of the other primate lentiviruses, consisting of gag, pol, vif, vpr, tat, rev, env, and nef. A unique feature is the absence of the highly conserved NF-kappa B binding site in the long terminal repeat. SIVsyk is genetically equidistant from other primate lentiviruses. Thus, SIVsyk represents a new group that is distinct from the four previously recognized primate lentivirus groups: human immunodeficiency virus type 1 (HIV-1), SIV from sooty mangabeys (SIVsmm) and HIV-2, SIV from African green monkeys (SIVagm), and SIV from mandrills (SIVmnd). The genetic differences between SIVsyk and SIVagm, isolates derived from monkeys of the same genus, underscore the potential for other distinct SIVs which have yet to be isolated and characterized.

Asunto(s)

Cercopithecus/microbiología , Lentivirus/clasificación , Enfermedades de los Monos/microbiología , Virus de la Inmunodeficiencia de los Simios/genética , África , Secuencia de Aminoácidos , Animales , Animales Salvajes/microbiología , Secuencia de Bases , Línea Celular , Clonación Molecular , Productos del Gen gag/genética , Productos del Gen pol/clasificación , Productos del Gen pol/genética , Genes Reguladores/genética , VIH-1/genética , Lentivirus/genética , Lentivirus/aislamiento & purificación , Lentivirus/patogenicidad , Macaca/microbiología , Datos de Secuencia Molecular , Filogenia , Reacción en Cadena de la Polimerasa , Secuencias Repetitivas de Ácidos Nucleicos/genética , Homología de Secuencia de Aminoácido , Virus de la Inmunodeficiencia de los Simios/aislamiento & purificación , Virus de la Inmunodeficiencia de los Simios/patogenicidad , Especificidad de la Especie , Transfección , Virulencia