RESUMEN
The aim the study was to evaluate the effects of autohemotransfusion in adjuvant-induced arthritis model by injections of high and low doses of Complete Freund ́s Adjuvant (CFA). Male Holtzman rats (200-230g) were distributed in six groups: control (C); control treated by autohemotransfusion (CT); CFA induced arthritis 0.5% w/v (AIA); CFA induced arthritis 0.5% w/v treated with autohemotransfusion (AIAT); CFA induced arthritis 0.1% w/v (AS) and CFA induced arthritis 0.1% w/v treated with autohemotransfusion (AST). The number of leukocytes, the weight of different organs and the paw volume were analyzed. The autohemotransfusion without erythrocytes promoted a reduction in the number of leukocytes in AIAT and AST when compared to AIA (p 0.05). The autohemotransfusion used in this work presented positive effects on AIA as they promoted a reduction in the number of leukocytes and an increase in thymus weight and body growth. However, other types of autohemotransfusion must be tested to determine the true efficacy of this alternative method of treatment.
Asunto(s)
Animales , Ratas , Adyuvante de Freund , Artritis Experimental/sangre , Transfusión Sanguínea/métodosRESUMEN
The aim of this study was to investigate some biochemical parameters of renal function and the vascular, glomerular, tubular, and interstitial manifestations in the progression of nephropathy induced by renal microembolism. Renal microembolism was induced by the arterial injection of polymethacrylate microspheres in the remnant kidney of nephrectomized rats. Animals 110-120 days old were randomly divided into three groups: the control group (C; normal), the nephrectomized group (S; nephrectomized that did not undergo renal microembolism), and the model group (M, nephrectomized animals that underwent renal arterial microembolism). The animals were evaluated 30, 60, and 90 days after the induction of a renal microembolism. Blood and urine samples were collected to determine serum creatinine (Cr) and urea (Ur) concentrations and urine total protein (Pt) concentrations. The kidneys were weighed and processed for histopathological analysis using hematoxylin and eosin (HE), periodic acid-Schiff (PAS), Mallory-Azan, and Picro-Sirius staining. The samples were also subjected to immunohistochemistry with a proliferating cell nuclear antigen (PCNA) and a vascular endothelial growth factor receptor (VEGFR). The data demonstrated evidence of the occurrence of vascular, glomerular, tubular, and interstitial abnormalities in the renal tissue, and changes in the biochemical parameters of renal function (serum Cr and Ur and of 24-h urine Pt) in this experimental model of nephropathy induced by renal microembolism, which may indicate the development of chronic kidney disease (CKD). Additionally, the findings indicate that this is a good reproducibility model that may be useful for studying the pathogenesis of CKD that is caused by atheroembolism and possible treatment alternatives.
RESUMEN
This study investigated the kinetics of cytokines that are involved in the development of interstitial fibrosis in mice that were subjected to UUO, the interstitial type I and III collagen deposition, and the effects of Thalido and Dexa treatment on these parameters. Inbred C57BL/6 mice were divided into the groups: Normal (not submitted surgery), Sham (sham surgery), Control (UUO treated with 0.5% carboxymethyl cellulose), Thalido (UUO treated with 5 mg/kg thalidomide), and Dexa (UUO treated with 1 mg/kg dexamethasone). The treatments began the day before surgery and were administered once daily by gavage for 1, 7, or 14 days. At the end of each treatment period, blood samples were collected for the determination of creatinine, urea, cytokines. The Control group exhibited a increase in creatinine concentration compared with the Normal and Sham groups within the first 24 h after UUO, which remained high until days 7 and 14. The urea concentration was higher on days 7 and 14 in the Control group compared with the Sham group. In the Thalido and Dexa groups, a reduction of serum creatinine concentration was seen on day 14. Treatment with Dexa reduced the serum concentration of urea on day 7. The serum concentrations of cytokines (TNF-α, IL-1ß, IL-6, IL-10 and IL-17) and chemokines (KC, MIG, bFGF) increased in UUO mice at all of the sampling times. The Dexa and Thalido groups exhibited alterations in the concentrations of these cytokines, suggesting the involvement of anti-inflammatory and immunomodulatory mechanisms that may have modified the fibrosis framework.
Asunto(s)
Antiinflamatorios/uso terapéutico , Citocinas/sangre , Dexametasona/uso terapéutico , Enfermedades Renales/tratamiento farmacológico , Riñón/patología , Talidomida/uso terapéutico , Obstrucción Ureteral/patología , Animales , Antiinflamatorios/administración & dosificación , Dexametasona/administración & dosificación , Modelos Animales de Enfermedad , Progresión de la Enfermedad , Fibrosis , Riñón/efectos de los fármacos , Riñón/inmunología , Enfermedades Renales/etiología , Enfermedades Renales/inmunología , Enfermedades Renales/patología , Pruebas de Función Renal , Masculino , Ratones Endogámicos C57BL , Talidomida/administración & dosificación , Obstrucción Ureteral/complicaciones , Obstrucción Ureteral/tratamiento farmacológico , Obstrucción Ureteral/inmunologíaRESUMEN
The aim of the present study was to evaluate histologically and radiographically the tissue response to dolomite [CaMg(CO3)2] and its osteogenic potential in the repair of bone cavities in the calvaria of rats. A bone defect 10 mm in diameter and 1 mm deep was made in the calvaria of male Wistar rats. The defects were filled with dolomite, inorganic bovine bone (positive control), or coagulum (negative control). The animals were euthanized 7, 15, 30, and 60 days after surgery, and specimens were collected for radiographic and microscopic analyses. The bone defects were processed for paraffin embedding and H&E staining. The histological study revealed that dolomite stimulated a moderate inflammatory response, with programmed cell death in the first 15 days, compared to bovine bone which showed a moderate to intense acute response. In the chronic phase, the inflammatory response was characterized by the occurrence of macrophages organized as epithelioid cells in the dolomite group, and giant cells in the bovine-bone group. Fibrosis developed in all three groups; however, encapsulation of the fragments, reabsorption, and osteoconductive activity occurred only in the defects filled with bovine bone. The radiographic analysis showed that the bovine bone was most efficient in the repair of the defects, followed by the dolomite and the coagulum. This study demonstrated that the dolomite stimulated a moderate acute inflammatory response with programmed cell death, and a chronic inflammatory response by means of the phagocytic mononuclear system. Although osteo-conductive activity was not shown, the dolomite favored the repair process, compared to the coagulum group.
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Regeneración Ósea/efectos de los fármacos , Huesos/efectos de los fármacos , Carbonato de Calcio/farmacología , Magnesio/farmacología , Osteogénesis/efectos de los fármacos , Animales , Huesos/lesiones , Masculino , Ratas , Ratas WistarRESUMEN
O objetivo deste trabalho foi estabelecer valores de referência de alguns parâmetros fisiológicos (bioquímicos, hematológicos e urinários) de ratos provenientes do Biotério Central da Universidade Estadual de Maringá, Estado do Paraná. Foram utilizados ratos machos, normais e saudáveis da linhagem Wistar, com peso de 200 a 220 g. Foi avaliado o perfil hematológico (leucócitos: totais, mononucleares e polimorfonucleares) e a determinação bioquímica de vários constituintes plasmáticos (aspartato aminotransferase, alanina aminotransferase, glicose, creatinina, fosfatase alcalina, fosfatase ácida, uréia, colesterol total, colesterol HDL, triglicerídeos, teste de tolerância à glicose GTT) e urinários (glicose, creatinina). É imprescindível que cada laboratório estabeleça seu conjunto de valores de referência dos animais normais, de acordo com a dieta, a linhagem, o sexo e a idade de cada espécie utilizada. Assim, através deste trabalho, o pesquisador pode identificar desvios dos parâmetros, facilitando a escolha dos animais para o estudo.
The present work was carried out to establish reference values for some physiological parameters (biochemical, hematological and urinary) in rats proceeding from the Central Biotery at the State University of Maringá, State of Paraná. Normal male Wistar rats weighing 200-220 g were used. The hematological profile (total leukocytes, mononuclear and polymorphonuclear cells count), the clinic chemistry (aspartate aminotransferase, alanine aminotransferase, glucose, creatinine, alkaline phosphatase, acid phosphatase, urea, cholesterol, HDL-c, triglycerides, glucose tolerance) and urinary parameters (glucose and creatinine) were determined. Each laboratory should establish a database of reference values for their animals, according to diet, strain, sex and age for each species used. Thus, in this work, a database is provided regarding reference values of some physiological parameters in rats for use in experimental investigations.
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Animales , Ratas , Estándares de Referencia , Plasma , Ratas Wistar , Valores de Referencia , Alternativas a las Pruebas en Animales , Animales de LaboratorioRESUMEN
The present study evaluated the effect of the crude extract of the leaves of Nectandra falcifolia (NEES) Castiglioni and its fractions in different experimental models of inflammation (paw edema, pleurisy, and ear edema). Carrageenan-induced edema of the paw and pleurisy were evaluated in Wistar rats (180-220 g), which were treated with different doses of the total extract (250, 500 mg.kg-1). Edema of the ear, induced by croton oil, and determination of myeloperoxidase activity were evaluated in Swiss mice (25-35 g). In this experiment, the crude extract of Nectandra falcifolia (Nf) (1.25, 2.5, 5.0, 7.5 mg) and the hexane, chloroform, ethyl-acetate and hydromethanol fractions (5.0 mg) were applied topically, immediately after application of the oil. The crude extract of Nf (500 mg.kg-1) significantly reduced edema of the paw compared to the control group. Similarly, at doses of 250 and 500 mg.kg-1 it significantly reduced the volume of pleural inflammatory exudate compared to the control animals. However, it did not change the number of migrated cells. At doses of 2.5, 5.0 and 7.5 mg, the crude extract significantly inhibited edema of the ear and the influx of neutrophils. The fractions from Nectandra falcifolia (hexane, chloroform, ethyl acetate and hydromethanol) also inhibited edema of the ear. Taken together, the results demonstrated that the crude extract and its fractions administered to animals orally or topically showed an anti-inflammatory effect.
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Antiinflamatorios no Esteroideos/uso terapéutico , Lauraceae/química , Extractos Vegetales/uso terapéutico , Hojas de la Planta/química , Administración Oral , Animales , Antiinflamatorios no Esteroideos/química , Antiinflamatorios no Esteroideos/farmacología , Carragenina/administración & dosificación , Carragenina/toxicidad , Aceite de Crotón/administración & dosificación , Aceite de Crotón/toxicidad , Evaluación Preclínica de Medicamentos/métodos , Oído/patología , Edema/inducido químicamente , Edema/metabolismo , Edema/prevención & control , Miembro Posterior/efectos de los fármacos , Miembro Posterior/patología , Indometacina/farmacología , Indometacina/uso terapéutico , Inflamación/inducido químicamente , Inflamación/metabolismo , Inflamación/prevención & control , Inyecciones Intradérmicas , Masculino , Óxido Nítrico/metabolismo , Peroxidasa/metabolismo , Fitoterapia/métodos , Extractos Vegetales/química , Extractos Vegetales/farmacología , Pleuresia/inducido químicamente , Pleuresia/metabolismo , Pleuresia/prevención & control , Ratas , Ratas Wistar , Solventes/químicaRESUMEN
Este estudo foi realizado para determinar a toxicidade aguda do extrato total de Cordia salicifolia (DL50) após administração oral e intraperitoneal em camundongos, assim como os efeitos do extrato sobre alguns parâmetros bioquímicos no plasma de ratos após um tratamento prolongado (90 dias). A DL50 do extrato administrado por v.o. foi maior que 2000 mg/Kg, enquanto a DL50 por via i.p. foi aproximadamente 920 mg/Kg. A administração oral diária do extrato nas doses de 20, 100, 200 e 400 mg/kg por um período de 90 dias não causou modificações no ganho de peso corporal, no peso dos órgãos, nos parâmetros hematológicos e bioquímicos dos animais. Estes resultados indicam que a administração do extrato por um período mais prolongado não provocou efeitos de toxicidade nos animais