RESUMEN
The article demonstrates that in conditions of absence of antigen stimulation in venous blood percentage of promocytes made up 26% (20.0; 36.0), monocytes proper--40% (29.5; 47.0), polymorphic nuclear monocytes--31.0% (24.5; 43.0). The molecule of L-selectin was expressed by 42.2% of monocytes; of LFA-1--99.72%; of ICAM-1--88.88%; of LFA-3--94.11%; of PECAM-1--97.50% of monocytes. The positive correlation is established between content of polymorphic nuclear monocytes and level of expression of molecules of LFA-1, ICAM-1, LFA-3, and PECAM-1. This occurrence can testify readiness to implementation of phase of sliding, solid adhesion and transmigration immediately by polymorphic nuclear monocytes primarily.
Asunto(s)
Moléculas de Adhesión Celular/biosíntesis , Diferenciación Celular/fisiología , Núcleo Celular/metabolismo , Regulación de la Expresión Génica/fisiología , Monocitos/metabolismo , Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Monocitos/citologíaRESUMEN
The article deals with results of detection of expression level of adhesion molecules on neutrophils and segmentation of their nuclei. It is established that in conditions of absence of antigen stimulation neutrophils of circulating pool express molecules of L-selectin in 53.34%, LFA-1 molecules in 65.64%, ICAM-1 in 40.51%, LE4-3 in 58.72% and PECAM-1 in 59.74%. The full readiness to realization of phase of sliding, strong adhesion and immediately transmigration itselfis detected in neutrophils with five segments in nucleus.
Asunto(s)
Antígenos CD58/sangre , Molécula 1 de Adhesión Intercelular/sangre , Neutrófilos/citología , Molécula-1 de Adhesión Celular Endotelial de Plaqueta/sangre , Adulto , Núcleo Celular/metabolismo , Núcleo Celular/ultraestructura , Femenino , Regulación de la Expresión Génica , Humanos , Molécula 1 de Adhesión Intercelular/biosíntesis , Masculino , Persona de Mediana Edad , Neutrófilos/metabolismoRESUMEN
Computer-aided urinodiagnosis of renal diseases consists in collection of a series of urinary portions in fixed periods at rest, during exercise and drug loading with assessment in each of these portions of protein, urea nitrogen, creatinine, phosphorus, and potassium. Concentrations of all elements are determined by open auto-analyzers using special programs; protein content is measured by two methods, sulfosalicylic acid test and Biuret method. Two data sets are formed including, respectively, information on protein content obtained by the said methods; these data are processed using special programs to reveal the regularities typical of various renal diseases. The method helps diagnose various morphologic types of glomerulonephritis and renal amyloidosis, chronic pyelonephritis and renal tuberculosis, permits assess the risk of oncological diseases of the kidneys and the type of changes in various parts of the nephron and mechanisms of proteinuria.