RESUMEN
beta-Catenin signaling is required for embryonic tooth morphogenesis and promotes continuous tooth development when activated in embryos. To determine whether activation of this pathway in the adult oral cavity could promote tooth development, we induced mutation of epithelial beta-catenin to a stabilized form in adult mice. This caused increased proliferation of the incisor tooth cervical loop, outpouching of incisor epithelium, abnormal morphology of the epithelial-mesenchymal junction, and enhanced expression of genes associated with embryonic tooth development. Ectopic dental-like structures were formed from the incisor region following implantation into immunodeficient mice. Thus, forced activation of beta-catenin signaling can initiate an embryonic-like program of tooth development in adult rodent incisor teeth.
Asunto(s)
Células Madre Adultas/fisiología , Papila Dental/citología , Órgano del Esmalte/citología , Odontogénesis/genética , beta Catenina/fisiología , Animales , Células Epiteliales/citología , Femenino , Factor 8 de Crecimiento de Fibroblastos/biosíntesis , Factor 8 de Crecimiento de Fibroblastos/genética , Incisivo/citología , Células Madre Mesenquimatosas/fisiología , Ratones , Ratones Desnudos , Isoformas de Proteínas/biosíntesis , Isoformas de Proteínas/genética , Transducción de Señal , Calcificación de Dientes , Regulación hacia ArribaRESUMEN
During the recent decade, the periodontal attachment apparatus has become one of the premier areas of the body for the development of novel tissue-engineering strategies. In the present review, we describe a developmental biology approach to characterize current concepts in periodontal regeneration and to discuss strategies for future applications in periodontal therapies. To decipher the developmental make-up of the periodontal region, we have followed the path of the migratory neural crest, since it gives rise to periodontal progenitor tissues, which in turn are subjected to the influence of diverse craniofacial extracellular matrices and peptide growth factors. Based on this developmental perspective, we have conducted a systematic analysis of the factors, progenitor cells, and matrices used in current periodontal tissue-engineering approaches. We propose that the developmental history of a tissue is a highly instructive design template for the discovery of novel bioengineering tools and approaches.