RESUMEN

We have employed a tandem Sonogashira/annulation reaction between 5-iodocytosine derivatives and terminal alkynes to yield the fluorescent bicyclic nucleobase pyrrolcytosine. Pyrrolocytosine bearing substituents only on the pyrrole ring are conveniently synthesized from 5-iodocytosine. Water soluble pyrrolocytosines are being investigated as reporter groups in SNP analysis.

Asunto(s)

Citosina/análogos & derivados , Química Farmacéutica/métodos , Citosina/síntesis química , Citosina/química , Modelos Químicos , Conformación Molecular , Sondas de Ácido Nucleico/química , Sondas de Ácido Nucleico/farmacología , Polimorfismo de Nucleótido Simple , Análisis de Secuencia de ADN/métodos , Agua/química